##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727067.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4772111
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.856265078494612	31.0	31.0	33.0	28.0	34.0
2	31.061010525530524	31.0	31.0	34.0	28.0	34.0
3	31.127830429761588	31.0	31.0	34.0	28.0	34.0
4	34.95248413123668	35.0	35.0	37.0	32.0	37.0
5	34.107249391307114	35.0	35.0	37.0	30.0	37.0
6	34.16781671675282	35.0	35.0	37.0	30.0	37.0
7	34.124057466391704	35.0	35.0	37.0	30.0	37.0
8	34.05848250386464	35.0	33.0	37.0	30.0	37.0
9	35.69193989829658	37.0	35.0	39.0	30.0	39.0
10	35.3318950460289	37.0	34.0	39.0	30.0	39.0
11	35.5415737395882	37.0	35.0	39.0	30.0	39.0
12	35.37148360547355	37.0	35.0	39.0	30.0	39.0
13	35.52483963595985	37.0	35.0	39.0	30.0	39.0
14	36.29979813964931	38.0	35.0	40.0	30.0	41.0
15	36.22840143492052	38.0	35.0	40.0	30.0	41.0
16	36.13296673107562	38.0	35.0	40.0	30.0	41.0
17	36.10073529303908	38.0	34.0	40.0	30.0	41.0
18	36.10302065480036	38.0	34.0	40.0	30.0	41.0
19	36.16782258417711	38.0	34.0	40.0	30.0	41.0
20	36.053872594329846	38.0	34.0	40.0	29.0	41.0
21	35.960026705162555	38.0	34.0	40.0	29.0	41.0
22	35.873302611779145	38.0	34.0	40.0	29.0	41.0
23	35.7595332547797	38.0	34.0	40.0	29.0	41.0
24	35.612143556593715	38.0	34.0	40.0	27.0	41.0
25	35.562998429835346	38.0	34.0	40.0	27.0	41.0
26	35.26571406239293	38.0	34.0	40.0	27.0	41.0
27	35.03837085935344	38.0	33.0	40.0	27.0	40.0
28	34.88185815459867	37.0	33.0	40.0	26.0	40.0
29	34.68640649808858	37.0	33.0	39.0	25.0	40.0
30	34.57121869126682	37.0	33.0	39.0	25.0	40.0
31	34.47108564742103	37.0	33.0	39.0	25.0	40.0
32	34.2923370810109	37.0	33.0	39.0	25.0	40.0
33	34.15315507120434	37.0	33.0	39.0	24.0	40.0
34	34.046507509988764	37.0	32.0	39.0	24.0	40.0
35	33.89235057608677	37.0	32.0	39.0	24.0	40.0
36	33.66641785993662	36.0	32.0	39.0	23.0	40.0
37	33.53487251239546	36.0	31.0	39.0	22.0	40.0
38	33.430045948218726	36.0	31.0	39.0	22.0	40.0
39	33.25390084178679	36.0	31.0	39.0	21.0	40.0
40	33.07333421204997	36.0	31.0	39.0	20.0	40.0
41	32.947343219803564	36.0	31.0	39.0	19.0	40.0
42	32.691328638415996	36.0	30.0	39.0	18.0	40.0
43	31.405360436921942	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	20.0
11	21.0
12	13.0
13	27.0
14	72.0
15	127.0
16	294.0
17	648.0
18	1268.0
19	2787.0
20	5157.0
21	9207.0
22	15446.0
23	23906.0
24	36118.0
25	51587.0
26	72270.0
27	97818.0
28	126009.0
29	159908.0
30	198124.0
31	242795.0
32	295753.0
33	365459.0
34	450686.0
35	559989.0
36	676796.0
37	710335.0
38	533606.0
39	135855.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.48635457138361	19.749289151069622	13.951289062639155	23.813067214907615
2	18.693907161840954	22.901122794503312	34.95237223107342	23.452597812582315
3	21.549561609107585	24.19260574617816	29.535503260506722	24.722329384207534
4	16.078670424891627	16.43920269247719	32.9130022331836	34.56912464944759
5	15.859333531847856	36.10647363399552	32.64999493934655	15.384197894810075
6	34.80409403720911	34.05258176098586	14.605695466848948	16.53762873495608
7	28.18111313839934	30.6083617920874	21.855107729053245	19.35541734046002
8	26.49806343565772	35.09023574682148	19.716033428392592	18.695667389128207
9	26.239771036340105	15.553787411902196	20.145759392436595	38.06068215932111
10	17.95815311085597	27.163890362147903	32.10937465620561	22.768581870790516
11	34.19733950027567	22.589688295180057	21.16084474983922	22.052127454705055
12	21.41517663776052	26.218920725020855	30.115498151656574	22.25040448556205
13	31.17341570638235	19.811609579073078	25.12248772084304	23.892486993701528
14	21.954623436043295	21.51265970133553	27.28951610723221	29.243200755388965
15	25.292810665971516	27.561785549414086	23.938504364211145	23.206899420403253
16	23.329528588081878	26.103919208920328	26.335892019276162	24.23066018372163
17	23.315677275738135	26.854698057107225	26.890929402103183	22.93869526505146
18	23.378773041951455	24.95838843648021	28.07084747190499	23.591991049663346
19	24.2033347505957	25.698480190423062	28.209213909735126	21.88897114924611
20	23.577993051712333	25.668137224804706	27.9766124467767	22.777257276706262
21	22.869333927898992	26.52981039208853	28.087863002348435	22.512992677664037
22	23.335982754801808	26.284258685516743	27.35189940049592	23.027859159185525
23	22.96845148824074	25.974668233827753	27.587686036640807	23.4691942412907
24	22.869753029634055	26.22021994039954	27.70340002569094	23.206627004275465
25	23.348576761940365	25.849817826953313	27.49544174475405	23.30616366635227
26	22.998354397037286	26.126634522960597	28.10207055116698	22.772940528835143
27	23.52501859240072	26.43530295083245	27.828920995341477	22.210757461425352
28	23.33133072554264	26.486181901468765	27.73001298586726	22.452474387121338
29	22.93840189383692	26.656190520295947	28.05234413030208	22.353063455565053
30	22.77555991467927	26.673646107561204	28.06447712553208	22.486316852227453
31	22.70383065272371	26.007043842861155	28.114413097264503	23.17471240715063
32	22.234352889109243	26.521197851433044	27.95586691089122	23.288582348566493
33	22.843035294023966	26.257687635513925	27.65352691921877	23.24575015124334
34	23.00239872878062	26.32750998457496	27.78602593275806	22.884065353886363
35	22.688093382572198	26.448106508838542	27.9661768135737	22.89762329501556
36	22.53076259123059	26.11519304559345	28.038450907784835	23.315593455391127
37	22.07199287694691	25.648355622909865	28.373501789878734	23.906149710264494
38	22.256921517542235	25.63999454329541	28.70566087000072	23.397423069161636
39	21.905274206739954	25.41642472272753	29.00336978750075	23.674931283031768
40	22.143617363468703	25.54974098465019	29.29263380503932	23.014007846841785
41	21.974027846376583	25.371119825167522	29.141379988856087	23.51347233959981
42	21.393760539098945	25.76687759358489	29.16847491602773	23.670886951288434
43	21.49428209025314	25.337067809193876	28.969087265572824	24.19956283498016
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5003.0
1	4181.0
2	3359.0
3	6081.0
4	8803.0
5	8803.0
6	11801.5
7	14800.0
8	14848.5
9	14897.0
10	20496.5
11	26096.0
12	26096.0
13	41458.0
14	56820.0
15	75616.0
16	94412.0
17	83248.0
18	72084.0
19	72084.0
20	73859.0
21	75634.0
22	61057.0
23	46480.0
24	50105.0
25	53730.0
26	53730.0
27	60723.5
28	67717.0
29	75490.5
30	83264.0
31	92847.5
32	102431.0
33	102431.0
34	115404.5
35	128378.0
36	146203.5
37	164029.0
38	184311.0
39	204593.0
40	204593.0
41	226876.5
42	249160.0
43	272226.5
44	295293.0
45	319690.0
46	344087.0
47	344087.0
48	370117.0
49	396147.0
50	405205.0
51	414263.0
52	419339.0
53	424415.0
54	424415.0
55	391763.0
56	359111.0
57	325203.0
58	291295.0
59	264832.0
60	238369.0
61	238369.0
62	209198.5
63	180028.0
64	153011.5
65	125995.0
66	104770.5
67	83546.0
68	83546.0
69	68905.5
70	54265.0
71	45168.0
72	36071.0
73	29477.0
74	22883.0
75	22883.0
76	18689.0
77	14495.0
78	11742.5
79	8990.0
80	7045.0
81	5100.0
82	5100.0
83	4104.0
84	3108.0
85	2400.5
86	1693.0
87	1263.0
88	833.0
89	833.0
90	570.0
91	307.0
92	195.0
93	83.0
94	57.0
95	31.0
96	31.0
97	22.0
98	13.0
99	6.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4772111.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.1202313455703
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.28319803737625	48.98558968476825
2	8.171667147094688	9.498783701362793
3	2.7212240791248044	4.744745190656104
4	1.2968192041618918	3.0148572863707006
5	0.7854216974146412	2.2824445378784737
6	0.5206404230973757	1.8155845102965047
7	0.36166615127766544	1.471408426748396
8	0.27305572883338364	1.2696049704023655
9	0.21658574730942085	1.1329212365799095
>10	1.254585327223125	13.361796319131805
>50	0.07748554358614725	3.0596922142773866
>100	0.03304816051090388	3.581783930166927
>500	0.002589049585190033	1.002777132631922
>1k	0.001690072500228808	1.839485798389463
>5k	1.797949468326532E-4	0.6219884662542629
>10k+	1.4383595746612256E-4	2.3165365940847082
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	39535	0.8284593547803057	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	29078	0.6093320126040656	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	24473	0.5128338381064481	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17756	0.3720785203864705	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	7587	0.15898624319509752	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6074	0.12728119693779125	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5543	0.1161540458719422	No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG	5531	0.11590258483090607	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5026	0.10532026602063532	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4897	0.10261705982949683	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0955086753011403E-5	0.0	0.0	0.0	4.1910173506022806E-5
2	4.1910173506022806E-5	0.0	0.0	0.0	4.1910173506022806E-5
3	6.286526025903422E-5	0.0	0.0	0.0	4.1910173506022806E-5
4	8.382034701204561E-5	0.0	0.0	0.0	6.286526025903422E-5
5	8.382034701204561E-5	2.0955086753011403E-5	0.0	0.0	6.286526025903422E-5
6	8.382034701204561E-5	2.0955086753011403E-5	0.0	0.0	8.382034701204561E-5
7	1.0477543376505702E-4	2.0955086753011403E-5	0.0	0.0	8.382034701204561E-5
8	1.2573052051806843E-4	2.0955086753011403E-5	0.0	2.0955086753011403E-5	1.2573052051806843E-4
9	1.2573052051806843E-4	2.0955086753011403E-5	0.0	2.0955086753011403E-5	1.8859578077710262E-4
10	2.0955086753011404E-4	2.0955086753011403E-5	0.0	1.0477543376505702E-4	1.8859578077710262E-4
11	2.0955086753011404E-4	2.0955086753011403E-5	0.0	1.6764069402409122E-4	1.8859578077710262E-4
12	2.5146104103613686E-4	2.0955086753011403E-5	0.0	3.14326301295171E-4	1.8859578077710262E-4
13	2.9337121454215965E-4	2.0955086753011403E-5	0.0	3.5623647480119387E-4	1.8859578077710262E-4
14	2.9337121454215965E-4	2.0955086753011403E-5	0.0	5.029220820722737E-4	2.7241612778914823E-4
15	2.9337121454215965E-4	2.0955086753011403E-5	0.0	7.124729496023877E-4	2.7241612778914823E-4
16	3.14326301295171E-4	2.0955086753011403E-5	0.0	0.0013411255521927298	3.5623647480119387E-4
17	3.3528138804818244E-4	2.0955086753011403E-5	0.0	0.0019488230680300605	3.5623647480119387E-4
18	3.3528138804818244E-4	2.0955086753011403E-5	0.0	0.0025355654971143796	3.7719156155420524E-4
19	3.3528138804818244E-4	2.0955086753011403E-5	0.0	0.0037719156155420525	3.7719156155420524E-4
20	3.3528138804818244E-4	2.0955086753011403E-5	0.0	0.0055950081630540445	3.7719156155420524E-4
21	3.7719156155420524E-4	2.0955086753011403E-5	0.0	0.009576474646126211	3.9814664830721666E-4
22	4.610119085662509E-4	2.0955086753011403E-5	0.0	0.014563785293342926	3.9814664830721666E-4
23	4.610119085662509E-4	2.0955086753011403E-5	0.0	0.018880533164463274	4.4005682181323945E-4
24	4.610119085662509E-4	2.0955086753011403E-5	0.0	0.026005262660487152	4.4005682181323945E-4
25	4.610119085662509E-4	2.0955086753011403E-5	0.0	0.03319285741677006	4.4005682181323945E-4
26	4.610119085662509E-4	2.0955086753011403E-5	0.0	0.043879951660805876	4.4005682181323945E-4
27	4.819669953192623E-4	2.0955086753011403E-5	0.0	0.0733218485487869	4.610119085662509E-4
28	4.819669953192623E-4	2.0955086753011403E-5	0.0	0.1589443330215915	4.610119085662509E-4
29	4.819669953192623E-4	2.0955086753011403E-5	0.0	0.29762509715302093	4.610119085662509E-4
30	4.819669953192623E-4	4.1910173506022806E-5	0.0	0.46413421649244957	4.610119085662509E-4
31	4.819669953192623E-4	4.1910173506022806E-5	0.0	0.8548208539155941	4.610119085662509E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	4445	0.0	14.275592	37
TATACAC	2110	0.0	12.71327	37
TCTTATA	6740	0.0	12.433976	37
ATAGGAC	475	0.0	12.073684	3
CTCTTAT	9810	0.0	11.579001	37
TATACTG	690	0.0	11.528986	5
GTATTAG	540	0.0	11.305555	1
GTTATAT	355	3.6379788E-12	10.943662	1
TCGTTAC	340	2.0008883E-11	10.882353	23
GTACTAC	375	1.8189894E-12	10.8533325	1
CGTAAAC	120	0.007167949	10.791666	3
TTACACA	895	0.0	10.748604	4
ATATACT	535	0.0	10.719625	4
ATACCCG	575	0.0	10.617392	5
TATACTC	475	0.0	10.515789	5
CGACCGA	345	2.9649527E-10	10.188406	18
TATACCG	200	3.2216893E-5	10.175	5
CGTACAC	255	4.2494503E-7	10.156862	3
GTTACAC	475	0.0	10.126316	3
ATTATAC	530	0.0	10.122642	3
>>END_MODULE