##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727066.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1644769
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31660737769255	33.0	31.0	34.0	31.0	34.0
2	32.4982578100633	34.0	31.0	34.0	31.0	34.0
3	32.61658445654071	34.0	31.0	34.0	31.0	34.0
4	36.13781023353432	37.0	35.0	37.0	35.0	37.0
5	35.93389466849144	37.0	35.0	37.0	35.0	37.0
6	35.9633754040841	37.0	35.0	37.0	35.0	37.0
7	35.919537637200115	37.0	35.0	37.0	35.0	37.0
8	35.91444330480451	37.0	35.0	37.0	35.0	37.0
9	37.73216178077286	39.0	37.0	39.0	35.0	39.0
10	37.59659927929089	39.0	37.0	39.0	35.0	39.0
11	37.65869675316108	39.0	37.0	39.0	35.0	39.0
12	37.583830920937835	39.0	37.0	39.0	35.0	39.0
13	37.61283742580265	39.0	37.0	39.0	35.0	39.0
14	38.91234331386353	40.0	38.0	41.0	36.0	41.0
15	38.82411694286553	40.0	38.0	41.0	35.0	41.0
16	38.7715059075165	40.0	38.0	41.0	35.0	41.0
17	38.7799295828168	40.0	38.0	41.0	35.0	41.0
18	38.7581909678502	40.0	38.0	41.0	35.0	41.0
19	38.78592738554776	40.0	38.0	41.0	35.0	41.0
20	38.76263961687021	40.0	38.0	41.0	35.0	41.0
21	38.67232237475293	40.0	38.0	41.0	35.0	41.0
22	38.65273056581198	40.0	38.0	41.0	35.0	41.0
23	38.57140546788029	40.0	38.0	41.0	34.0	41.0
24	38.4925329939949	40.0	38.0	41.0	34.0	41.0
25	38.47078890713529	40.0	38.0	41.0	34.0	41.0
26	38.30267897802062	40.0	38.0	41.0	34.0	41.0
27	38.21787862003722	40.0	38.0	41.0	34.0	41.0
28	38.123663566129956	40.0	38.0	41.0	34.0	41.0
29	38.03485535050819	40.0	38.0	41.0	33.0	41.0
30	37.96175693972832	40.0	37.0	41.0	33.0	41.0
31	37.91917466829688	40.0	37.0	41.0	33.0	41.0
32	37.77337060705789	40.0	37.0	41.0	33.0	41.0
33	37.65463964848559	40.0	37.0	41.0	33.0	41.0
34	37.58430393568945	40.0	37.0	41.0	33.0	41.0
35	37.50351690723743	40.0	37.0	41.0	32.0	41.0
36	37.36811917053398	40.0	37.0	41.0	32.0	41.0
37	37.30647403981958	40.0	37.0	41.0	32.0	41.0
38	37.233332461883705	40.0	36.0	41.0	32.0	41.0
39	37.132835066808774	39.0	36.0	41.0	31.0	41.0
40	37.00939888823294	39.0	36.0	41.0	31.0	41.0
41	36.92732657291084	39.0	36.0	41.0	31.0	41.0
42	36.719831781849	39.0	35.0	41.0	30.0	41.0
43	35.42555884747341	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	1.0
12	2.0
13	1.0
14	3.0
15	16.0
16	34.0
17	46.0
18	57.0
19	156.0
20	273.0
21	485.0
22	891.0
23	1458.0
24	2434.0
25	3701.0
26	5531.0
27	7980.0
28	11541.0
29	15742.0
30	20777.0
31	27409.0
32	35610.0
33	47720.0
34	66746.0
35	92190.0
36	137037.0
37	227938.0
38	455136.0
39	483852.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.98090613332329	17.532492404708503	11.153845920004573	26.332755541963643
2	18.00806070639707	21.17470599214844	34.21392304937654	26.60331025207795
3	20.489928980908566	23.839031499256127	28.553310525672604	27.117728994162704
4	14.46616515753884	14.963985824149168	33.42317371010762	37.14667530820437
5	15.057190401813264	36.774586583283124	32.856163996281545	15.312059018622067
6	35.483827820198464	33.03777004552007	16.18221160539869	15.29619052888278
7	29.578135288298846	30.085562167088508	21.381361151626763	18.954941392985887
8	28.093428317289543	32.75207643140161	20.45016655834345	18.704328692965394
9	28.29369960158539	13.65249466642428	18.84477394697979	39.20903178501054
10	17.620650681037883	25.718991542277365	31.807262904395696	24.85309487228906
11	36.777748121468726	21.238483945161903	20.604352343702974	21.37941558966639
12	22.588035158736577	25.266830782924533	28.672962586235514	23.47217147210338
13	30.64393844971543	18.56126909006675	24.742805828660437	26.051986631557376
14	23.568051197462985	20.263818201826517	24.31083027464647	31.857300326064024
15	27.113169083318084	25.74616861091132	21.53603332747638	25.604628978294215
16	25.527657683236978	24.8235466500159	24.80731336740904	24.841482299338082
17	25.469898812538418	25.52644170701174	24.420146537294904	24.583512943154936
18	25.820464758273047	23.0035950337099	26.17005792302749	25.00588228498956
19	25.78258709885704	25.05683168882682	25.209071912225973	23.951509300090166
20	25.141159640046716	23.81683993314563	25.649437702194046	25.39256272461361
21	25.639892288825965	24.61664829529253	25.628218917063734	24.115240498817766
22	25.790490944321053	24.179626439943846	24.0987032221546	25.931179393580493
23	23.81915028797357	24.34232405888	25.428859614936812	26.40966603820962
24	24.43370467220625	26.012528203048575	24.64066382574088	24.913103299004298
25	25.30373566136035	24.4593009717474	23.923602645721072	26.31336072117118
26	25.106504317627582	24.983265127200234	24.905321051162808	25.004909504009376
27	25.998483677647133	24.420146537294904	23.793067597942326	25.788302187115637
28	24.57767625727382	25.218921319650356	25.08212399431166	25.12127842876416
29	24.672036012351885	24.695625951121404	25.368060803675167	25.264277232851544
30	24.369744322759	25.86022718083816	25.759665947011406	24.010362549391434
31	26.26265451257897	24.530678776168568	23.866147769078818	25.340518942173638
32	23.72065621372971	25.190589073602432	24.777217955834526	26.31153675683333
33	24.03243251787941	24.847379784030462	25.247800754999638	25.872386943090486
34	24.686810123488463	24.75952550175739	25.325988026282108	25.227676348472034
35	25.016947668639183	25.397183434269493	25.58730131708465	23.998567580006675
36	24.818621946303708	25.263182854248832	24.690032460485334	25.22816273896213
37	25.01567089360269	25.06522192478093	25.20986229677237	24.70924488484401
38	24.773387630725043	24.670759237315394	25.65101847128685	24.904834660672716
39	25.130519848075934	23.841585049329115	25.23272264980675	25.7951724527882
40	25.062303581840368	24.67580553865011	25.651140068909374	24.610750810600152
41	23.596505041133437	24.984906695104296	26.03210542027482	25.386482843487446
42	25.302215691078807	25.17380860169422	25.77766239514485	23.746313312082123
43	24.280309271393126	23.561241730601683	25.93245616861699	26.2259928293882
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	24.0
1	67.5
2	111.0
3	682.0
4	1253.0
5	1253.0
6	1733.0
7	2213.0
8	2133.0
9	2053.0
10	2571.0
11	3089.0
12	3089.0
13	4683.5
14	6278.0
15	7816.5
16	9355.0
17	8353.0
18	7351.0
19	7351.0
20	7993.5
21	8636.0
22	7895.5
23	7155.0
24	8931.5
25	10708.0
26	10708.0
27	12648.0
28	14588.0
29	16649.0
30	18710.0
31	21446.5
32	24183.0
33	24183.0
34	29006.0
35	33829.0
36	41456.0
37	49083.0
38	58517.5
39	67952.0
40	67952.0
41	74360.0
42	80768.0
43	87536.5
44	94305.0
45	107746.5
46	121188.0
47	121188.0
48	148669.5
49	176151.0
50	179982.0
51	183813.0
52	160695.5
53	137578.0
54	137578.0
55	132492.0
56	127406.0
57	122082.0
58	116758.0
59	109877.0
60	102996.0
61	102996.0
62	87668.0
63	72340.0
64	63721.5
65	55103.0
66	47477.5
67	39852.0
68	39852.0
69	32967.5
70	26083.0
71	22429.5
72	18776.0
73	14207.5
74	9639.0
75	9639.0
76	7703.5
77	5768.0
78	4815.0
79	3862.0
80	3126.5
81	2391.0
82	2391.0
83	2016.5
84	1642.0
85	1367.5
86	1093.0
87	743.0
88	393.0
89	393.0
90	286.0
91	179.0
92	122.5
93	66.0
94	55.0
95	44.0
96	44.0
97	23.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1644769.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.22988459067541
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.05283437092118	43.900698387123676
2	8.287053399728075	8.656636853291232
3	2.7530828886761824	4.313796046323608
4	1.3681528188515002	2.858338553240844
5	0.8126783203218592	2.122304743987732
6	0.531581323352992	1.6658658701571165
7	0.36895783169793805	1.3489437477886401
8	0.2734977264005494	1.1427803748570253
9	0.21398123215384743	1.0058593553969375
>10	1.1501397562017928	10.869574268780845
>50	0.0866049982645852	3.1691429598121474
>100	0.08410311462606215	9.417561637853508
>500	0.011981332461438062	4.291174416952695
>1k	0.005118239109740531	4.416574162055206
>5k	2.3264723226093325E-4	0.8207486223787903
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	7815	0.4751427100097339	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	5694	0.34618843132379074	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	4267	0.25942852765342733	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3653	0.22209805753877898	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	3105	0.18878030896739906	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2779	0.1689598964961037	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2644	0.160752056975782	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	2445	0.14865309353471523	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	2380	0.14470117080270847	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	2290	0.13922927778916067	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	2280	0.13862128967654425	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	2219	0.13491256218958406	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2204	0.13400058002065943	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1835	0.11156581866511345	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	1760	0.1070059078204903	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	1731	0.10524274229390268	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	1678	0.10202040529703563	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	6.079881126164221E-5	0.0
9	0.0	0.0	0.0	6.079881126164221E-5	0.0
10	0.0	0.0	0.0	1.2159762252328442E-4	0.0
11	0.0	0.0	0.0	1.2159762252328442E-4	0.0
12	0.0	0.0	0.0	3.0399405630821105E-4	0.0
13	0.0	0.0	0.0	3.6479286756985327E-4	0.0
14	0.0	0.0	0.0	5.471893013547799E-4	0.0
15	0.0	0.0	0.0	6.079881126164221E-4	1.2159762252328442E-4
16	0.0	0.0	0.0	0.0010335797914479176	1.8239643378492663E-4
17	0.0	0.0	0.0	0.0015807690928026975	1.8239643378492663E-4
18	0.0	0.0	0.0	0.0018847631491109085	2.4319524504656884E-4
19	0.0	0.0	0.0	0.0026143488842506153	2.4319524504656884E-4
20	0.0	0.0	0.0	0.003404733430651964	2.4319524504656884E-4
21	0.0	0.0	0.0	0.005532691824809441	3.0399405630821105E-4
22	0.0	0.0	0.0	0.009241419311769616	3.6479286756985327E-4
23	0.0	0.0	0.0	0.015503696871718764	4.255916788314955E-4
24	0.0	0.0	0.0	0.025961092408721225	4.255916788314955E-4
25	0.0	0.0	0.0	0.03264896164750187	4.255916788314955E-4
26	0.0	0.0	0.0	0.04170798452548656	4.255916788314955E-4
27	0.0	0.0	0.0	0.057880468321083386	4.863904900931377E-4
28	0.0	0.0	0.0	0.09302218123031258	4.863904900931377E-4
29	0.0	0.0	0.0	0.12670472266926236	4.863904900931377E-4
30	0.0	0.0	0.0	0.1780189193740884	4.863904900931377E-4
31	0.0	0.0	0.0	0.31366106729881216	5.471893013547799E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	480	0.0	17.34375	5
GTAATAC	115	1.2429155E-6	16.086956	3
CAAGACG	565	0.0	15.38938	4
TGCACCG	145	5.349466E-8	15.310345	5
CGAGCCG	655	0.0	15.251907	15
ACGAGTC	85	5.3658965E-4	15.235294	26
GACGGAC	525	0.0	15.15238	7
TCGCGGT	75	0.004105405	14.8	17
AGGCCCG	740	0.0	14.75	10
TACCGCA	665	0.0	14.744361	28
TCGTTTA	340	0.0	14.691176	30
AAGGCGT	140	5.9988633E-7	14.535715	6
ACGGACC	550	0.0	14.463636	8
TAACGCC	405	0.0	14.160494	4
CTTATAC	720	0.0	14.131945	37
TTTGCGC	355	0.0	14.070422	17
TGGATAC	715	0.0	13.972028	24
CGGACCA	570	0.0	13.95614	9
ATACCGC	690	0.0	13.942029	27
TTTCGGA	505	0.0	13.920793	30
>>END_MODULE