FastQCFastQC Report
Thu 9 Feb 2017
SRR2727065.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727065.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3204155
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC133470.41655288211712604No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG113190.3532600638857983No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA71150.22205542490921942No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC48270.15064814280208041No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG46580.14537374128280312No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG38770.12099914017892394No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT38050.11875205787485311No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG35680.11135541195728671No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC34260.10692366630203594No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG33880.10573770619710969No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT32670.10196135954721293No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC32250.10065056153650495No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGACA700.002593849815.85714328
TCTAGAT7300.015.4589042
CGTTATT6400.015.32031252
TATTCCG855.36787E-415.2352945
GTATACG855.36787E-415.2352941
GTTATTC7700.015.1363633
TAATACC2750.014.7999994
CTTATAC11700.014.70512837
CTAGCGG7300.014.6986329
GCGTTAT6950.014.6402871
TACTTAC2700.014.38888931
TAGATAA7200.014.3888894
CAAGACG8000.014.3375014
TCTAGCG7500.014.30666628
CTAGATA7250.014.2896563
GTACTAG1956.7484507E-1014.230771
AAGACGG8350.014.1796415
TCGTTTA6150.014.13821130
TATGCCG800.00630186313.8755
CGGTATA800.00630186313.87534
Produced by FastQC (version 0.11.5)