##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727065.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3204155
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29697970291699	33.0	31.0	34.0	31.0	34.0
2	32.493123460007396	34.0	31.0	34.0	31.0	34.0
3	32.61406579893919	34.0	31.0	34.0	31.0	34.0
4	36.137303594863546	37.0	35.0	37.0	35.0	37.0
5	35.91990930526145	37.0	35.0	37.0	35.0	37.0
6	35.93976383789174	37.0	35.0	37.0	35.0	37.0
7	35.900886505178434	37.0	35.0	37.0	35.0	37.0
8	35.89048251411059	37.0	35.0	37.0	35.0	37.0
9	37.72545335665721	39.0	37.0	39.0	35.0	39.0
10	37.57500776335726	39.0	37.0	39.0	35.0	39.0
11	37.64725052314885	39.0	37.0	39.0	35.0	39.0
12	37.56152683000666	39.0	37.0	39.0	35.0	39.0
13	37.594302710074885	39.0	37.0	39.0	35.0	39.0
14	38.894459537694026	40.0	38.0	41.0	36.0	41.0
15	38.80023157431523	40.0	38.0	41.0	35.0	41.0
16	38.74994312072918	40.0	38.0	41.0	35.0	41.0
17	38.744553244147056	40.0	38.0	41.0	35.0	41.0
18	38.723146040063604	40.0	38.0	41.0	35.0	41.0
19	38.74346184875576	40.0	38.0	41.0	35.0	41.0
20	38.71683735649493	40.0	38.0	41.0	35.0	41.0
21	38.61908771579402	40.0	38.0	41.0	34.0	41.0
22	38.60271803330363	40.0	38.0	41.0	34.0	41.0
23	38.53419575519911	40.0	38.0	41.0	34.0	41.0
24	38.45384508552177	40.0	38.0	41.0	34.0	41.0
25	38.43273374727502	40.0	38.0	41.0	34.0	41.0
26	38.27244250044083	40.0	38.0	41.0	34.0	41.0
27	38.19920134949776	40.0	38.0	41.0	34.0	41.0
28	38.097543970251124	40.0	38.0	41.0	33.0	41.0
29	38.02527811544697	40.0	38.0	41.0	33.0	41.0
30	37.963962417548466	40.0	38.0	41.0	33.0	41.0
31	37.94144165934544	40.0	38.0	41.0	33.0	41.0
32	37.81347999706631	40.0	37.0	41.0	33.0	41.0
33	37.71939154004722	40.0	37.0	41.0	33.0	41.0
34	37.66324319516378	40.0	37.0	41.0	33.0	41.0
35	37.60081519152475	40.0	37.0	41.0	33.0	41.0
36	37.474524484614506	40.0	37.0	41.0	32.0	41.0
37	37.43130092021141	40.0	37.0	41.0	32.0	41.0
38	37.39025608935897	40.0	37.0	41.0	32.0	41.0
39	37.30629666792025	40.0	37.0	41.0	31.0	41.0
40	37.21236644294674	39.0	36.0	41.0	31.0	41.0
41	37.155670683846445	39.0	36.0	41.0	31.0	41.0
42	36.966839307087206	39.0	36.0	41.0	31.0	41.0
43	35.64652490282149	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	4.0
13	7.0
14	2.0
15	14.0
16	46.0
17	96.0
18	153.0
19	276.0
20	485.0
21	949.0
22	1644.0
23	2814.0
24	4560.0
25	6820.0
26	10216.0
27	14829.0
28	20946.0
29	28379.0
30	38585.0
31	52164.0
32	68965.0
33	92230.0
34	128785.0
35	180211.0
36	268882.0
37	445545.0
38	904490.0
39	932054.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.7024566539384	16.957044837094333	10.968227192504733	27.37227131646253
2	18.830924221830717	20.190190549458435	33.301291604182694	27.67759362452815
3	20.646785189855045	22.309064324291427	28.203941444780295	28.840209041073233
4	14.944595376940253	14.64994670981897	32.68637128977843	37.71908662346235
5	15.695058447547014	35.79293136567988	32.814829494827805	15.697180691945304
6	37.09361750601953	32.4421883460694	15.224169866938396	15.240024280972674
7	30.4438767787451	29.300330352308173	20.567950052353897	19.68784281659283
8	28.507266346353404	32.452269006961274	19.615031108045645	19.425433538639673
9	28.223821881276027	13.29867000816128	17.794988070177627	40.682520040385064
10	17.645463468527584	25.20527252895069	31.641696484720622	25.507567517801107
11	38.25866726172735	20.33447195906565	19.638250958521045	21.768609820685953
12	23.060744564479556	24.204540666728043	28.106942391987904	24.627772376804494
13	31.745093480184323	17.933963868789117	23.463721324342924	26.857221326683632
14	23.850313109072438	19.421750820419113	23.144791684547094	33.583144385961354
15	27.630873038289344	25.282734449488242	20.52606693496413	26.560325577258283
16	26.53317333275076	24.112098197496685	23.54689457906999	25.80783389068257
17	26.439825788702482	24.56813106731728	23.303991223895224	25.688051920085016
18	26.818334319032633	22.453564200233757	24.71334876121786	26.014752719515755
19	26.597464854228335	24.46860404693281	23.838609555405405	25.095321543433446
20	26.14058932854372	22.959969165037272	24.19174478138542	26.707696725033593
21	26.521532198036613	23.85905176247716	24.3198596821939	25.29955635729233
22	26.843582785476983	23.305021136617924	22.87670228188087	26.974693796024223
23	25.111269585897062	23.349775525840666	24.00061794763362	27.538336940628653
24	25.50148166989425	24.94083463502858	23.458977483923217	26.098706211153953
25	26.490291512114737	23.258924739908025	22.73376287976081	27.517020868216424
26	26.2779110249036	24.045715641097264	23.646327971025123	26.030045362974015
27	27.356073598187354	23.23876341812428	22.63439190675857	26.7707710769298
28	25.739578765696415	24.139999469438898	23.734182647219	26.386239117645683
29	25.734054688365575	23.729220340464177	24.177232374838294	26.35949259633195
30	25.525481757280783	24.70682598064076	24.458180081800037	25.30951218027842
31	27.288255405871435	23.42686293266087	22.58380134544053	26.70108031602716
32	25.07837479772358	23.899967386097114	23.602010514472617	27.41964730170669
33	25.343780185415497	23.643050975998353	23.938417461077883	27.074751377508267
34	26.09546042560363	23.599357708974754	24.092467436812516	26.2127144286091
35	26.18609274520115	24.037913271985907	24.556146628362235	25.219847354450703
36	25.924057980965344	24.01494309732207	23.796351924298296	26.264646997414292
37	26.077140462930164	23.706999193235035	24.279006477526835	25.936853866307967
38	25.81054911513332	23.300152458292438	24.615787937849447	26.273510488724796
39	26.113468293512643	22.520851831450102	24.34607564240806	27.019604232629195
40	26.145738892157215	23.180214440312657	24.86103200375762	25.81301466377251
41	24.894987914130244	23.43650666088251	25.226432553980693	26.442072871006552
42	26.17273508928251	23.51446793304319	25.28326501058781	25.029531967086488
43	25.17262741658877	21.9768082380534	25.32951745467994	27.521046890677887
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17.0
1	45.5
2	74.0
3	349.0
4	624.0
5	624.0
6	936.5
7	1249.0
8	1179.0
9	1109.0
10	1488.0
11	1867.0
12	1867.0
13	2859.5
14	3852.0
15	4979.0
16	6106.0
17	5542.5
18	4979.0
19	4979.0
20	5507.0
21	6035.0
22	5734.0
23	5433.0
24	7273.0
25	9113.0
26	9113.0
27	11564.0
28	14015.0
29	17014.5
30	20014.0
31	24831.5
32	29649.0
33	29649.0
34	38372.0
35	47095.0
36	60834.0
37	74573.0
38	92644.0
39	110715.0
40	110715.0
41	126084.0
42	141453.0
43	159160.0
44	176867.0
45	205861.5
46	234856.0
47	234856.0
48	288225.0
49	341594.0
50	354291.5
51	366989.0
52	332623.0
53	298257.0
54	298257.0
55	289138.5
56	280020.0
57	269500.5
58	258981.0
59	243598.0
60	228215.0
61	228215.0
62	197437.5
63	166660.0
64	147994.0
65	129328.0
66	110892.0
67	92456.0
68	92456.0
69	75466.0
70	58476.0
71	49445.0
72	40414.0
73	30571.0
74	20728.0
75	20728.0
76	16625.5
77	12523.0
78	10428.0
79	8333.0
80	6603.5
81	4874.0
82	4874.0
83	4038.5
84	3203.0
85	2688.0
86	2173.0
87	1453.5
88	734.0
89	734.0
90	508.0
91	282.0
92	197.0
93	112.0
94	102.0
95	92.0
96	92.0
97	51.0
98	10.0
99	8.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3204155.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.345385274909425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.66967125850972	41.62036450059779
2	8.889102248422516	8.950505548897905
3	3.0384631748962785	4.58917797551333
4	1.437064653603389	2.8939829460246753
5	0.879441149328056	2.2137901744765065
6	0.5928483420756144	1.790830691483286
7	0.41720675267324464	1.4703104291839846
8	0.3238013975989555	1.3041524891738838
9	0.24151717272368986	1.094334760015291
>10	1.3361810641626422	12.221899166982764
>50	0.08695961517022431	3.004045782497915
>100	0.06734059543908646	6.937439189154228
>500	0.01279797911264988	4.387502412864886
>1k	0.00741911832579893	6.5324316553620285
>5k	6.182598604832441E-5	0.22146526718302942
>10k+	1.2365197209664882E-4	0.7677670105884193
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	13347	0.41655288211712604	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	11319	0.3532600638857983	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	7115	0.22205542490921942	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	4827	0.15064814280208041	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	4658	0.14537374128280312	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	3877	0.12099914017892394	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	3805	0.11875205787485311	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	3568	0.11135541195728671	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	3426	0.10692366630203594	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	3388	0.10573770619710969	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	3267	0.10196135954721293	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	3225	0.10065056153650495	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	3.120947644542789E-5	0.0	0.0	0.0	0.0
3	3.120947644542789E-5	0.0	0.0	0.0	0.0
4	3.120947644542789E-5	0.0	0.0	6.241895289085578E-5	3.120947644542789E-5
5	6.241895289085578E-5	0.0	0.0	6.241895289085578E-5	3.120947644542789E-5
6	9.362842933628367E-5	0.0	0.0	6.241895289085578E-5	9.362842933628367E-5
7	9.362842933628367E-5	0.0	0.0	9.362842933628367E-5	9.362842933628367E-5
8	9.362842933628367E-5	0.0	0.0	9.362842933628367E-5	9.362842933628367E-5
9	9.362842933628367E-5	0.0	0.0	9.362842933628367E-5	9.362842933628367E-5
10	9.362842933628367E-5	0.0	0.0	1.2483790578171156E-4	9.362842933628367E-5
11	9.362842933628367E-5	0.0	0.0	1.2483790578171156E-4	9.362842933628367E-5
12	9.362842933628367E-5	0.0	0.0	1.2483790578171156E-4	9.362842933628367E-5
13	9.362842933628367E-5	0.0	0.0	1.2483790578171156E-4	1.2483790578171156E-4
14	1.2483790578171156E-4	0.0	0.0	1.8725685867256733E-4	2.496758115634231E-4
15	1.2483790578171156E-4	0.0	0.0	2.1846633511799524E-4	4.0572319379056257E-4
16	1.2483790578171156E-4	0.0	0.0	5.929800524631299E-4	4.3693267023599047E-4
17	1.2483790578171156E-4	0.0	0.0	8.738653404719809E-4	4.3693267023599047E-4
18	1.2483790578171156E-4	0.0	0.0	0.001154750628480832	4.6814214668141837E-4
19	1.2483790578171156E-4	0.0	0.0	0.0017789401573893896	4.6814214668141837E-4
20	1.2483790578171156E-4	0.0	0.0	0.002309501256961664	4.6814214668141837E-4
21	1.2483790578171156E-4	0.0	0.0	0.004182069843687337	5.61770576017702E-4
22	1.2483790578171156E-4	0.0	0.0	0.005773753142404159	5.61770576017702E-4
23	1.2483790578171156E-4	0.0	0.0	0.009830985080309785	6.866084817994135E-4
24	1.2483790578171156E-4	0.0	0.0	0.015011758170250815	6.866084817994135E-4
25	1.2483790578171156E-4	0.0	0.0	0.01788303000323018	7.178179582448415E-4
26	1.2483790578171156E-4	0.0	0.0	0.022720498852271502	7.178179582448415E-4
27	1.2483790578171156E-4	0.0	0.0	0.032052132309454445	7.178179582448415E-4
28	1.2483790578171156E-4	0.0	0.0	0.05474142168528052	7.178179582448415E-4
29	1.2483790578171156E-4	0.0	0.0	0.08311083577417447	7.178179582448415E-4
30	1.2483790578171156E-4	0.0	0.0	0.12362073620033986	7.178179582448415E-4
31	1.2483790578171156E-4	0.0	0.0	0.25788390386857063	7.178179582448415E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGACA	70	0.0025938498	15.857143	28
TCTAGAT	730	0.0	15.458904	2
CGTTATT	640	0.0	15.3203125	2
TATTCCG	85	5.36787E-4	15.235294	5
GTATACG	85	5.36787E-4	15.235294	1
GTTATTC	770	0.0	15.136363	3
TAATACC	275	0.0	14.799999	4
CTTATAC	1170	0.0	14.705128	37
CTAGCGG	730	0.0	14.69863	29
GCGTTAT	695	0.0	14.640287	1
TACTTAC	270	0.0	14.388889	31
TAGATAA	720	0.0	14.388889	4
CAAGACG	800	0.0	14.337501	4
TCTAGCG	750	0.0	14.306666	28
CTAGATA	725	0.0	14.289656	3
GTACTAG	195	6.7484507E-10	14.23077	1
AAGACGG	835	0.0	14.179641	5
TCGTTTA	615	0.0	14.138211	30
TATGCCG	80	0.006301863	13.875	5
CGGTATA	80	0.006301863	13.875	34
>>END_MODULE