FastQCFastQC Report
Thu 9 Feb 2017
SRR2727064.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727064.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5134343
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT325450.6338688319031277No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT260980.5083026202183999No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT233380.4545469595623043No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202410.39422765483334476No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC164750.3208784454018752No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG119610.23296067286505792No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA90080.1754460113007643No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81620.15896873270835238No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78780.1534373531335947No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC66350.12922782914970815No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65670.12790341432194927No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG59350.1155941470992491No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG56280.10961480368569065No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG53910.1049988284771781No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT53000.1032264498106184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG13600.016.7316175
CAAGACG14300.015.2657354
ATACCGT11350.014.8325996
ACCGTCG11000.014.6318188
TACCGTC11300.014.5707977
GTATTAC4000.013.8750011
TATGCGA800.00630254313.87500112
CGTCGTA11350.013.85462610
TCTAGCG10050.013.80596928
ACGGACC14750.013.6711878
CTATACT6500.013.6615394
CCGTCGT11350.013.5286349
CGCAAGA16550.013.5256812
GACGGAC15550.013.443737
GTCCTAT14900.013.4093951
TATACTG5250.013.3904755
CTAGCGG10400.013.34134629
CGGACCA15050.013.2757469
CGAGCCG15250.013.22295115
CCTATTC16250.013.2061543
Produced by FastQC (version 0.11.5)