FastQCFastQC Report
Thu 9 Feb 2017
SRR2727062.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727062.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5904107
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT411910.6976668952646015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT332930.5638956069054982No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT302010.5115252823161911No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247290.4188440351775467No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC135110.2288407035983596No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG107560.18217827014313936No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106770.1808402185123No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96250.1630221132509963No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82750.1401566739898176No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA74400.12601397637271816No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65990.11176965458112463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG13950.015.25089555
TCTAGCG11900.014.61344628
GCGTTAT10750.014.4558131
CTAGCGG12050.014.43153529
CGCAATA11900.014.30252236
CAAGACG15450.014.2491924
GTACTAG3650.014.1917821
CGGTCCA13450.013.75464510
TAGCGGC12750.013.63921530
ACCGTAT1951.0286385E-813.282058
TACTTAC10500.013.21428731
GTACCGT2800.013.2142856
CGCTTCG13450.013.2044632
GCGCAAT12950.013.14285835
GTATTAG15650.013.1214061
ACGGACC16500.013.1181828
TACCGTA1703.7390237E-713.0588247
GCGCAAG16050.013.0249221
GTAAACG13650.013.01098827
ATACCGT10950.012.8401826
Produced by FastQC (version 0.11.5)