##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727062.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5904107
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14771988380292	33.0	31.0	34.0	30.0	34.0
2	32.310735560856195	34.0	31.0	34.0	30.0	34.0
3	32.42010112621604	34.0	31.0	34.0	30.0	34.0
4	35.982022852905615	37.0	35.0	37.0	35.0	37.0
5	35.76868068278573	37.0	35.0	37.0	35.0	37.0
6	35.80948380508686	37.0	35.0	37.0	35.0	37.0
7	35.76778757566555	37.0	35.0	37.0	35.0	37.0
8	35.77430066900888	37.0	35.0	37.0	35.0	37.0
9	37.54772466013912	39.0	37.0	39.0	35.0	39.0
10	37.404612585781386	39.0	37.0	39.0	34.0	39.0
11	37.47056328755559	39.0	37.0	39.0	35.0	39.0
12	37.40150186980012	39.0	37.0	39.0	35.0	39.0
13	37.43300485577243	39.0	37.0	39.0	35.0	39.0
14	38.66829022576996	40.0	38.0	41.0	35.0	41.0
15	38.57161904416705	40.0	38.0	41.0	34.0	41.0
16	38.513027795736086	40.0	38.0	41.0	34.0	41.0
17	38.55314105926603	40.0	38.0	41.0	34.0	41.0
18	38.55098527177776	40.0	38.0	41.0	34.0	41.0
19	38.59725035471071	40.0	38.0	41.0	34.0	41.0
20	38.563470817856114	40.0	38.0	41.0	34.0	41.0
21	38.45990917847526	40.0	38.0	41.0	34.0	41.0
22	38.42216054011216	40.0	38.0	41.0	34.0	41.0
23	38.32542804525731	40.0	38.0	41.0	34.0	41.0
24	38.236105477085694	40.0	38.0	41.0	34.0	41.0
25	38.19743442996545	40.0	38.0	41.0	34.0	41.0
26	37.99160211696705	40.0	37.0	41.0	33.0	41.0
27	37.87752965859189	40.0	37.0	41.0	33.0	41.0
28	37.76317316064902	40.0	37.0	41.0	33.0	41.0
29	37.641663506437126	40.0	37.0	41.0	33.0	41.0
30	37.53387243828745	40.0	37.0	41.0	33.0	41.0
31	37.4382701736266	39.0	36.0	41.0	33.0	41.0
32	37.24990383812489	39.0	36.0	41.0	32.0	41.0
33	37.081389107616104	39.0	36.0	41.0	31.0	41.0
34	36.97090499884233	39.0	35.0	41.0	31.0	41.0
35	36.84214683101102	39.0	35.0	41.0	31.0	41.0
36	36.63262945607185	39.0	35.0	40.0	30.0	41.0
37	36.532178024551385	39.0	35.0	40.0	30.0	41.0
38	36.419438197851086	39.0	35.0	40.0	30.0	41.0
39	36.25873785824003	39.0	35.0	40.0	30.0	41.0
40	36.07709463937561	39.0	35.0	40.0	29.0	41.0
41	35.937769420506775	39.0	35.0	40.0	28.0	41.0
42	35.6610708444139	39.0	35.0	40.0	27.0	41.0
43	34.430514724750076	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	24.0
10	28.0
11	39.0
12	38.0
13	32.0
14	29.0
15	77.0
16	161.0
17	206.0
18	359.0
19	649.0
20	1287.0
21	2566.0
22	4424.0
23	7496.0
24	12306.0
25	19406.0
26	29062.0
27	41976.0
28	58422.0
29	78774.0
30	100752.0
31	126678.0
32	158767.0
33	205176.0
34	274129.0
35	368407.0
36	539958.0
37	881307.0
38	1574051.0
39	1417517.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.963683754376405	18.370703647477935	12.102592314129808	24.563020284015856
2	17.49099398096952	22.245447109952448	35.39724127628446	24.86631763279358
3	20.609196276422498	25.316004604930093	29.022085812469182	25.052713306178227
4	14.477362961070996	15.660573224706123	33.66922381318631	36.19284000103657
5	14.34870675616143	37.780548353883155	32.886361984970804	14.984382904984614
6	33.665548405542104	33.75460505712379	16.777846336456978	15.802000200877117
7	27.850545391538468	31.312084960519854	22.510940265818352	18.326429382123326
8	27.39061131514046	33.361996996328145	21.16187934940881	18.08551233912258
9	27.958605763750555	13.92320633755452	20.46043542232551	37.65775247636942
10	17.81920618986072	26.472843395283995	32.1536008747809	23.554349540074394
11	34.580606347412065	22.261622291059428	21.668306485637878	21.48946487589063
12	21.688512758999796	26.626109587783553	29.88841496266921	21.796962690547446
13	29.64035035272904	19.38606464957359	26.688608455097445	24.284976542599924
14	22.70255264682703	21.245990291165114	26.639693352440936	29.41176370956692
15	25.867857747158034	26.643876880957613	23.79462296330334	23.69364240858101
16	23.427979879090945	25.82200830709877	26.981675636976092	23.768336176834193
17	23.44684810082202	26.72698851833139	26.451858003250955	23.37430537759563
18	23.568915671751885	24.142787385120222	28.682085199336665	23.606211743791228
19	23.765998820820826	25.689337947296686	28.39992567885372	22.144737553028765
20	23.437210741607494	24.650891320228443	28.619958953995923	23.29193898416814
21	24.210384398521235	25.365156830660418	28.382073698867583	22.04238507195076
22	24.05135950280034	24.968449928160176	27.165835578521868	23.814354990517618
23	22.503199891194384	25.350218077009785	28.23656481835441	23.910017213441424
24	22.59591501305786	26.803172774477158	27.743314950084745	22.85759726238024
25	23.27701716787992	25.621165063573542	27.16121167858238	23.940606089964156
26	23.202679084237463	26.10913047476951	27.87669667910829	22.811493761884734
27	23.7587326923445	25.815284174219745	26.99722413567369	23.428758997762067
28	22.539869280824348	26.42521553217108	28.158229517181855	22.87668566982272
29	22.73915428700733	26.130725611849513	28.40429551835697	22.725824582786185
30	22.630399482936202	26.859845189120048	28.414678121517785	22.095077206425966
31	23.61625559970373	26.019514890228105	27.15989056431396	23.204338945754202
32	21.827602379157423	26.636559940394033	27.731695919467587	23.804141760980958
33	22.03732080058847	26.12672839431941	28.237242312851034	23.59870849224108
34	22.622438922600825	26.101407037507958	28.29360985497045	22.982544184920766
35	22.81332299702563	26.40150322478912	28.395301778914238	22.389871999271016
36	22.327728816567856	26.467609750297548	27.81502774255277	23.389633690581828
37	22.440260652457688	26.25179387839685	28.239884541387884	23.068060927757575
38	22.285995155575602	25.712254198645113	28.640927408666546	23.360823237112744
39	22.4267785119748	25.221849807261282	28.41315375890037	23.938217921863544
40	22.492732601221487	25.696688762585097	28.81045346908516	23.00012516710825
41	21.188386321589363	26.117717717514267	29.095492341178776	23.598403619717597
42	22.289873811568796	26.354891603421144	28.648820219552256	22.706414365457807
43	21.355354840283212	25.312735693983868	28.727680578959696	24.604228886773225
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1333.0
1	1842.5
2	2352.0
3	8526.5
4	14701.0
5	14701.0
6	20192.5
7	25684.0
8	24411.0
9	23138.0
10	30465.0
11	37792.0
12	37792.0
13	55706.0
14	73620.0
15	89108.0
16	104596.0
17	91288.0
18	77980.0
19	77980.0
20	80667.5
21	83355.0
22	68975.5
23	54596.0
24	60669.5
25	66743.0
26	66743.0
27	74914.5
28	83086.0
29	90406.5
30	97727.0
31	106881.0
32	116035.0
33	116035.0
34	134644.0
35	153253.0
36	180071.5
37	206890.0
38	235930.0
39	264970.0
40	264970.0
41	289152.5
42	313335.0
43	335783.0
44	358231.0
45	399980.0
46	441729.0
47	441729.0
48	499097.5
49	556466.0
50	556535.5
51	556605.0
52	499048.5
53	441492.0
54	441492.0
55	420421.0
56	399350.0
57	379272.0
58	359194.0
59	333840.0
60	308486.0
61	308486.0
62	260417.0
63	212348.0
64	185890.0
65	159432.0
66	135607.0
67	111782.0
68	111782.0
69	92371.0
70	72960.0
71	63391.0
72	53822.0
73	40199.0
74	26576.0
75	26576.0
76	21178.5
77	15781.0
78	13407.5
79	11034.0
80	9078.0
81	7122.0
82	7122.0
83	6014.5
84	4907.0
85	4168.5
86	3430.0
87	2307.0
88	1184.0
89	1184.0
90	863.5
91	543.0
92	386.0
93	229.0
94	204.5
95	180.0
96	180.0
97	98.0
98	16.0
99	19.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5904107.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.20461189309003
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.06347576060595	37.917110481167995
2	9.24824830934692	8.546234476486008
3	3.0255351743152348	4.193810354943837
4	1.5085963088423133	2.788164278136291
5	0.8860426076133213	2.0469627402757484
6	0.5857347789135827	1.6238188879192192
7	0.4331828875662448	1.4010533039108477
8	0.30954645171398965	1.144197893146242
9	0.24421627912156332	1.0155526555307741
>10	1.477442695937706	12.731971948168605
>50	0.11646532998643153	3.7128994585801163
>100	0.08005519912521199	7.55103630587495
>500	0.011349505729424066	3.7229656010235908
>1k	0.009561308049611635	7.930563138785433
>5k	2.919483373908895E-4	0.9023032651141722
>10k+	2.5545479521702836E-4	2.7713552109365214
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	41191	0.6976668952646015	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	33293	0.5638956069054982	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	30201	0.5115252823161911	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24729	0.4188440351775467	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	13511	0.2288407035983596	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	10756	0.18217827014313936	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10677	0.1808402185123	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9625	0.1630221132509963	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8275	0.1401566739898176	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	7440	0.12601397637271816	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6599	0.11176965458112463	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.081208724706378E-5	1.693736241568793E-5
2	0.0	0.0	0.0	6.774944966275171E-5	1.693736241568793E-5
3	0.0	0.0	0.0	8.468681207843963E-5	1.693736241568793E-5
4	0.0	0.0	0.0	8.468681207843963E-5	1.693736241568793E-5
5	0.0	0.0	0.0	8.468681207843963E-5	1.693736241568793E-5
6	0.0	0.0	0.0	1.0162417449412756E-4	6.774944966275171E-5
7	0.0	0.0	0.0	1.185615369098155E-4	8.468681207843963E-5
8	5.081208724706378E-5	0.0	0.0	1.185615369098155E-4	1.0162417449412756E-4
9	5.081208724706378E-5	0.0	0.0	1.3549889932550343E-4	1.863109865725672E-4
10	5.081208724706378E-5	0.0	0.0	2.8793516106669477E-4	1.863109865725672E-4
11	5.081208724706378E-5	0.0	0.0	7.791186711216447E-4	1.863109865725672E-4
12	5.081208724706378E-5	0.0	0.0	0.001354988993255034	1.863109865725672E-4
13	5.081208724706378E-5	0.0	0.0	0.0017276109664001685	1.863109865725672E-4
14	5.081208724706378E-5	0.0	0.0	0.002709977986510068	2.37123073819631E-4
15	5.081208724706378E-5	0.0	0.0	0.00376009445628272	2.540604362353189E-4
16	5.081208724706378E-5	0.0	0.0	0.007469376825318376	2.7099779865100685E-4
17	5.081208724706378E-5	0.0	0.0	0.011161721831938344	2.7099779865100685E-4
18	5.081208724706378E-5	0.0	0.0	0.013380516308393462	3.048725234823827E-4
19	5.081208724706378E-5	0.0	0.0	0.017835042623719388	3.048725234823827E-4
20	5.081208724706378E-5	0.0	0.0	0.022458942563202192	3.048725234823827E-4
21	5.081208724706378E-5	0.0	0.0	0.032502798475705134	3.048725234823827E-4
22	6.774944966275171E-5	0.0	0.0	0.047831111461902706	3.048725234823827E-4
23	6.774944966275171E-5	0.0	0.0	0.07508332758874459	4.403714228078861E-4
24	6.774944966275171E-5	0.0	0.0	0.11830747647358017	4.57308785223574E-4
25	6.774944966275171E-5	0.0	0.0	0.13866618609723705	4.74246147639262E-4
26	6.774944966275171E-5	0.0	0.0	0.16835738241193798	4.74246147639262E-4
27	6.774944966275171E-5	0.0	0.0	0.22255694214213936	4.74246147639262E-4
28	6.774944966275171E-5	0.0	0.0	0.33771406920640157	4.74246147639262E-4
29	6.774944966275171E-5	0.0	0.0	0.45598767095515036	4.74246147639262E-4
30	6.774944966275171E-5	0.0	0.0	0.6328984213870108	4.74246147639262E-4
31	6.774944966275171E-5	0.0	0.0	1.0073326923106238	4.911835100549498E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	1395	0.0	15.2508955	5
TCTAGCG	1190	0.0	14.613446	28
GCGTTAT	1075	0.0	14.455813	1
CTAGCGG	1205	0.0	14.431535	29
CGCAATA	1190	0.0	14.302522	36
CAAGACG	1545	0.0	14.249192	4
GTACTAG	365	0.0	14.191782	1
CGGTCCA	1345	0.0	13.754645	10
TAGCGGC	1275	0.0	13.639215	30
ACCGTAT	195	1.0286385E-8	13.28205	8
TACTTAC	1050	0.0	13.214287	31
GTACCGT	280	0.0	13.214285	6
CGCTTCG	1345	0.0	13.20446	32
GCGCAAT	1295	0.0	13.142858	35
GTATTAG	1565	0.0	13.121406	1
ACGGACC	1650	0.0	13.118182	8
TACCGTA	170	3.7390237E-7	13.058824	7
GCGCAAG	1605	0.0	13.024922	1
GTAAACG	1365	0.0	13.010988	27
ATACCGT	1095	0.0	12.840182	6
>>END_MODULE