FastQCFastQC Report
Thu 9 Feb 2017
SRR2727061.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727061.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5552372
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT521640.93949036555908No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT404370.728283335482565No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT373250.6722352176691331No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279890.5040908642288378No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC123570.22255353207602085No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG100130.18033734050960565No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99670.17950886576043537No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95230.1715122833988789No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69260.12473948071202723No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68170.12277635576290638No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA67120.12088527209632208No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA65470.11791356919168962No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT65390.11776948662661652No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA58600.10554047891603804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG15200.015.9440795
TCTAGCG9900.015.6969728
CTAGCGG10200.015.59803929
CAAGACG15450.015.4466014
GTACTAC4250.014.81
CGCAATA11350.014.18061636
CGCTTCG11750.014.01276632
GTCCTAT12750.013.9294131
CGAGCCG14950.013.85953115
CGGTCCA11900.013.83613510
ATTACGG4150.013.81927725
CTTATAC41900.013.77565637
GACGGAC16250.013.7753847
GCGCAAT12000.013.72083435
ACGGACC16200.013.7037048
TAGCGGC11650.013.65665330
CATGGGT41800.013.587324
GTAAACG12700.013.40157527
CGAACGA6650.013.35338316
GCCGGTC12500.013.328
Produced by FastQC (version 0.11.5)