##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727060.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4639915
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24060052824244	33.0	31.0	34.0	30.0	34.0
2	32.414758028972514	34.0	31.0	34.0	31.0	34.0
3	32.52278651656334	34.0	31.0	34.0	31.0	34.0
4	36.06325977954337	37.0	35.0	37.0	35.0	37.0
5	35.86711114319982	37.0	35.0	37.0	35.0	37.0
6	35.90134517550429	37.0	35.0	37.0	35.0	37.0
7	35.86538632712022	37.0	35.0	37.0	35.0	37.0
8	35.87047844626464	37.0	35.0	37.0	35.0	37.0
9	37.66161923224887	39.0	37.0	39.0	35.0	39.0
10	37.52268586816784	39.0	37.0	39.0	35.0	39.0
11	37.59354837319218	39.0	37.0	39.0	35.0	39.0
12	37.52115006417144	39.0	37.0	39.0	35.0	39.0
13	37.54856241978571	39.0	37.0	39.0	35.0	39.0
14	38.80400115088315	40.0	38.0	41.0	35.0	41.0
15	38.71497408896499	40.0	38.0	41.0	35.0	41.0
16	38.6717668319355	40.0	38.0	41.0	35.0	41.0
17	38.70915523236956	40.0	38.0	41.0	35.0	41.0
18	38.7069181224225	40.0	38.0	41.0	35.0	41.0
19	38.76065078778383	40.0	38.0	41.0	35.0	41.0
20	38.71959766504343	40.0	38.0	41.0	35.0	41.0
21	38.62502028593196	40.0	38.0	41.0	34.0	41.0
22	38.58444217189324	40.0	38.0	41.0	34.0	41.0
23	38.498499433718074	40.0	38.0	41.0	34.0	41.0
24	38.4208755979366	40.0	38.0	41.0	34.0	41.0
25	38.37737760282246	40.0	38.0	41.0	34.0	41.0
26	38.18290873862991	40.0	38.0	41.0	34.0	41.0
27	38.074814301555094	40.0	37.0	41.0	33.0	41.0
28	37.97427258904527	40.0	37.0	41.0	33.0	41.0
29	37.86822991369454	40.0	37.0	41.0	33.0	41.0
30	37.77406310244908	40.0	37.0	41.0	33.0	41.0
31	37.68311380704172	40.0	37.0	41.0	33.0	41.0
32	37.52275160213064	40.0	36.0	41.0	33.0	41.0
33	37.36874856543708	40.0	36.0	41.0	32.0	41.0
34	37.2735800117028	40.0	36.0	41.0	32.0	41.0
35	37.156236913822774	39.0	36.0	41.0	32.0	41.0
36	36.95342975032948	39.0	35.0	41.0	31.0	41.0
37	36.87546582211097	39.0	35.0	41.0	31.0	41.0
38	36.783932248758866	39.0	35.0	41.0	31.0	41.0
39	36.62978459734715	39.0	35.0	41.0	30.0	41.0
40	36.47173105541804	39.0	35.0	41.0	30.0	41.0
41	36.34118599155372	39.0	35.0	41.0	30.0	41.0
42	36.083039021188966	39.0	35.0	40.0	29.0	41.0
43	34.85426176988156	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	8.0
10	28.0
11	33.0
12	30.0
13	15.0
14	25.0
15	60.0
16	95.0
17	140.0
18	207.0
19	405.0
20	765.0
21	1472.0
22	2774.0
23	4621.0
24	7787.0
25	12224.0
26	18636.0
27	27719.0
28	39172.0
29	53917.0
30	70752.0
31	90215.0
32	114126.0
33	149803.0
34	202264.0
35	275741.0
36	403777.0
37	665932.0
38	1202598.0
39	1294573.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.63247710356763	18.34693092438116	12.374946523804855	24.64564544824636
2	17.612434710549653	21.692466349060275	36.04492754716412	24.650171393225953
3	20.19655532482815	24.70073266428372	29.620348648628266	25.482363362259868
4	14.213988833847171	15.419808337006174	34.14913850792525	36.2170643212214
5	14.055343686252872	38.0244896727634	33.10534783503577	14.814818805947954
6	33.51212252810666	34.467721930250875	16.60879563526487	15.411359906377594
7	27.953378456286377	31.661097239927887	22.06378780645766	18.321736497328075
8	27.534513024484287	33.72475573367185	20.627662360194098	18.11306888164977
9	28.19693895254547	13.719432360291083	20.139162032063087	37.94446665510036
10	17.46635444830347	26.849026329146113	32.382705286627015	23.301913935923395
11	34.997580774647815	22.203165359710255	21.58477041066485	21.214483454977085
12	21.59472748961996	26.474773783571464	30.038804590170294	21.89169413663828
13	29.82828349226225	19.705942889039992	26.421389184931186	24.044384433766567
14	22.519162527761825	21.161745419905323	26.852259146988683	29.46683290534417
15	25.57109343597889	27.178450467303822	23.756792958491697	23.493663138225592
16	23.439222485756744	26.126319124380508	26.474148772121904	23.96030961774084
17	23.161351016128528	26.71988603239499	26.32936163701275	23.789401314463735
18	23.147579212119187	24.87401170064538	28.432482060554985	23.545927026680445
19	23.816319911032853	25.365658638143156	28.586299533504384	22.231721917319604
20	23.55737120184314	24.76650973132051	28.451361716755585	23.224757350080765
21	24.268181636948093	25.2162593495786	28.48756927659235	22.027989736880958
22	24.18658531460167	24.78707045279924	27.50778839698572	23.518555835613366
23	22.90794120150908	25.298265162185086	28.407977301308325	23.385816334997518
24	22.766537749075145	26.16881990295081	27.999219813293998	23.065422534680053
25	23.250296611037054	25.471910584568896	27.640204615817314	23.63758818857673
26	23.09654379444451	25.94564771121885	28.129092882089434	22.82871561224721
27	23.513792817325317	25.984333764734913	27.38246282528883	23.119410592650944
28	22.565478031386352	26.404190594008725	28.063078741744192	22.96725263286073
29	22.764059255395843	26.284274604168395	28.3950244778191	22.556641662616663
30	22.97380447702167	26.515916778647885	28.138144772048623	22.37213397228182
31	23.335578345724006	26.049184090656833	27.65128240495785	22.96395515866131
32	22.206915428407633	26.576672201969213	27.800811006236103	23.415601363387044
33	22.173488091915477	26.024550018696463	28.238922480260953	23.563039409127107
34	22.784512216279822	26.081404508487765	28.441296877205723	22.692786398026687
35	22.880160520181946	26.181470996774724	28.288794083512304	22.649574399531026
36	22.257067209205342	26.30218441501622	28.05954419423632	23.38120418154212
37	22.313124270595473	26.044528832963536	28.46093085756959	23.1814160388714
38	22.299158497515577	25.50507929563365	28.730806491067185	23.464955715783585
39	22.068680137459413	25.340162481424766	28.91708145515597	23.67407592595985
40	22.392112786548893	25.417211306672645	29.21245324537195	22.978222661406512
41	21.28349333985644	25.880344790798972	29.442479010930157	23.393682858414433
42	21.778653272743142	26.05666267593264	29.162775611191154	23.00190844013306
43	21.060234939648677	25.387792664305277	29.02813952410766	24.523832871938385
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1708.0
1	2006.5
2	2305.0
3	6311.0
4	10317.0
5	10317.0
6	14073.0
7	17829.0
8	16930.0
9	16031.0
10	22366.5
11	28702.0
12	28702.0
13	42475.5
14	56249.0
15	68830.5
16	81412.0
17	70374.5
18	59337.0
19	59337.0
20	61394.5
21	63452.0
22	52099.0
23	40746.0
24	47211.5
25	53677.0
26	53677.0
27	60492.0
28	67307.0
29	74572.5
30	81838.0
31	90680.5
32	99523.0
33	99523.0
34	115430.5
35	131338.0
36	151646.0
37	171954.0
38	195559.0
39	219164.0
40	219164.0
41	238512.5
42	257861.0
43	274242.5
44	290624.0
45	316716.0
46	342808.0
47	342808.0
48	370252.5
49	397697.0
50	401856.0
51	406015.0
52	382234.0
53	358453.0
54	358453.0
55	342057.0
56	325661.0
57	307373.0
58	289085.0
59	266255.5
60	243426.0
61	243426.0
62	206290.0
63	169154.0
64	146525.5
65	123897.0
66	104418.0
67	84939.0
68	84939.0
69	69948.5
70	54958.0
71	46701.5
72	38445.0
73	29470.5
74	20496.0
75	20496.0
76	16440.5
77	12385.0
78	10428.0
79	8471.0
80	6813.5
81	5156.0
82	5156.0
83	4328.0
84	3500.0
85	2939.0
86	2378.0
87	1630.5
88	883.0
89	883.0
90	647.5
91	412.0
92	289.0
93	166.0
94	146.5
95	127.0
96	127.0
97	73.0
98	19.0
99	14.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4639915.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.59196011659131
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.08576314652085	42.86557378511856
2	8.686065082824307	8.96262246646376
3	2.9662884458374275	4.591099055758505
4	1.410842485698543	2.9115251701180713
5	0.8361298411415807	2.156878870823413
6	0.5689493526024164	1.7611927384694588
7	0.3968149698398117	1.4330723468349362
8	0.2934339815016103	1.2111067416386936
9	0.21451577686895437	0.9960560464142424
>10	1.3575361792590244	12.88397403493477
>50	0.10286546677041504	3.672035440403016
>100	0.0673905285222368	7.037089768877329
>500	0.008867112735486923	3.113857466852981
>1k	0.004204592822075532	3.4937948891180786
>5k	1.665185276069417E-4	0.5264360586159259
>10k+	1.665185276069417E-4	2.383685119558098
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	35980	0.7754452398373677	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	29239	0.6301624059923512	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	26397	0.5689112839351582	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19369	0.41744299195136114	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7019	0.15127432291324303	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6886	0.1484078910928325	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5399	0.11635989021350607	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5207	0.11222188337501873	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4694	0.10116564635343536	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.465635685136473E-5
2	0.0	0.0	0.0	0.0	6.465635685136473E-5
3	0.0	0.0	0.0	0.0	6.465635685136473E-5
4	0.0	0.0	0.0	2.155211895045491E-5	1.0776059475227455E-4
5	0.0	0.0	0.0	2.155211895045491E-5	1.0776059475227455E-4
6	0.0	0.0	0.0	2.155211895045491E-5	1.2931271370272945E-4
7	2.155211895045491E-5	0.0	0.0	6.465635685136473E-5	1.5086483265318437E-4
8	6.465635685136473E-5	0.0	0.0	1.0776059475227455E-4	1.5086483265318437E-4
9	6.465635685136473E-5	0.0	0.0	1.0776059475227455E-4	1.939690705540942E-4
10	6.465635685136473E-5	0.0	0.0	1.939690705540942E-4	1.939690705540942E-4
11	8.620847580181964E-5	0.0	0.0	5.388029737613728E-4	1.939690705540942E-4
12	8.620847580181964E-5	0.0	0.0	7.758762822163768E-4	2.155211895045491E-4
13	8.620847580181964E-5	0.0	0.0	0.0010776059475227455	2.3707330845500402E-4
14	1.2931271370272945E-4	0.0	0.0	0.0015517525644327536	2.3707330845500402E-4
15	1.2931271370272945E-4	0.0	0.0	0.002694014868806864	2.586254274054589E-4
16	1.2931271370272945E-4	0.0	0.0	0.005215612786010088	3.663860221577335E-4
17	1.2931271370272945E-4	0.0	0.0	0.007801867060064678	3.663860221577335E-4
18	1.2931271370272945E-4	0.0	0.0	0.009202754791844247	4.0949026005864333E-4
19	1.2931271370272945E-4	0.0	0.0	0.011681248471146563	4.0949026005864333E-4
20	1.2931271370272945E-4	0.0	0.0	0.013771804009340688	4.0949026005864333E-4
21	1.2931271370272945E-4	0.0	0.0	0.019504667650161695	4.0949026005864333E-4
22	1.2931271370272945E-4	0.0	0.0	0.02883673515570867	4.0949026005864333E-4
23	1.2931271370272945E-4	0.0	0.0	0.04444046927583803	5.172508548109178E-4
24	1.2931271370272945E-4	0.0	0.0	0.0682124564781898	5.388029737613728E-4
25	1.2931271370272945E-4	0.0	0.0	0.07717813796157903	5.388029737613728E-4
26	1.2931271370272945E-4	0.0	0.0	0.09021716992660427	5.603550927118277E-4
27	1.2931271370272945E-4	0.0	0.0	0.12379537125141302	5.603550927118277E-4
28	1.2931271370272945E-4	0.0	0.0	0.20957280467422357	5.603550927118277E-4
29	1.2931271370272945E-4	0.0	0.0	0.3122255472352403	5.819072116622826E-4
30	1.5086483265318437E-4	0.0	0.0	0.46386625617064103	5.819072116622826E-4
31	1.5086483265318437E-4	0.0	0.0	0.8496707375027345	6.034593306127375E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTTAC	1320	0.0	15.136365	31
TACGCGT	110	1.4536799E-5	15.136364	5
CAAGACG	820	0.0	15.115853	4
CTTATAC	4630	0.0	14.544276	37
AGTGTAC	1555	0.0	14.514468	27
AAGACGG	885	0.0	14.423728	5
GTCTAAT	320	0.0	13.875001	1
CTACAGT	1820	0.0	13.417582	23
GGAGTGT	1820	0.0	12.807692	6
GTCCTAT	975	0.0	12.71282	1
TCTTATA	6890	0.0	12.566037	37
TACGGAT	1215	0.0	12.333333	27
CGCAATA	765	0.0	12.333333	36
GTGTACT	1705	0.0	12.260997	28
GTATAGG	470	0.0	12.202128	1
TAGTACT	335	0.0	12.149253	4
AACGCCG	770	0.0	12.012986	5
TTACGGA	1205	0.0	11.975102	26
GGCGCAA	1210	0.0	11.925619	34
CATGGGT	3235	0.0	11.894899	4
>>END_MODULE