##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727059.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3846167
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24790733215693	33.0	31.0	34.0	30.0	34.0
2	32.41531919960834	34.0	31.0	34.0	31.0	34.0
3	32.523446849811776	34.0	31.0	34.0	31.0	34.0
4	36.066146633778516	37.0	35.0	37.0	35.0	37.0
5	35.87005556440997	37.0	35.0	37.0	35.0	37.0
6	35.90653421965297	37.0	35.0	37.0	35.0	37.0
7	35.86823297064324	37.0	35.0	37.0	35.0	37.0
8	35.87556572556522	37.0	35.0	37.0	35.0	37.0
9	37.66812777500301	39.0	37.0	39.0	35.0	39.0
10	37.52934414964301	39.0	37.0	39.0	35.0	39.0
11	37.59409614819117	39.0	37.0	39.0	35.0	39.0
12	37.523644189136874	39.0	37.0	39.0	35.0	39.0
13	37.55164401337748	39.0	37.0	39.0	35.0	39.0
14	38.80971003079169	40.0	38.0	41.0	35.0	41.0
15	38.72181733138473	40.0	38.0	41.0	35.0	41.0
16	38.679979574469854	40.0	38.0	41.0	35.0	41.0
17	38.72094711436087	40.0	38.0	41.0	35.0	41.0
18	38.71761236576571	40.0	38.0	41.0	35.0	41.0
19	38.76309635021048	40.0	38.0	41.0	35.0	41.0
20	38.72798945027608	40.0	38.0	41.0	35.0	41.0
21	38.63255807665138	40.0	38.0	41.0	34.0	41.0
22	38.59288429233572	40.0	38.0	41.0	34.0	41.0
23	38.50172886408728	40.0	38.0	41.0	34.0	41.0
24	38.4178092110925	40.0	38.0	41.0	34.0	41.0
25	38.37786918768738	40.0	38.0	41.0	34.0	41.0
26	38.185202046609	40.0	38.0	41.0	34.0	41.0
27	38.07239519240844	40.0	37.0	41.0	33.0	41.0
28	37.96394306331472	40.0	37.0	41.0	33.0	41.0
29	37.85217360556627	40.0	37.0	41.0	33.0	41.0
30	37.74440085414908	40.0	37.0	41.0	33.0	41.0
31	37.64968369808175	40.0	37.0	41.0	33.0	41.0
32	37.477410367256546	40.0	36.0	41.0	33.0	41.0
33	37.312206932252295	40.0	36.0	41.0	32.0	41.0
34	37.20807936836856	39.0	36.0	41.0	32.0	41.0
35	37.08209419923784	39.0	36.0	41.0	31.0	41.0
36	36.87317659373605	39.0	35.0	41.0	31.0	41.0
37	36.77940531443382	39.0	35.0	41.0	31.0	41.0
38	36.671017665119585	39.0	35.0	41.0	31.0	41.0
39	36.50729232506025	39.0	35.0	41.0	30.0	41.0
40	36.33195386471778	39.0	35.0	41.0	30.0	41.0
41	36.190753027624645	39.0	35.0	41.0	30.0	41.0
42	35.91432535300729	39.0	35.0	40.0	28.0	41.0
43	34.68251534579752	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	19.0
11	39.0
12	29.0
13	28.0
14	24.0
15	48.0
16	106.0
17	124.0
18	190.0
19	374.0
20	691.0
21	1351.0
22	2304.0
23	4011.0
24	6664.0
25	10595.0
26	16325.0
27	24033.0
28	33802.0
29	45903.0
30	59845.0
31	75424.0
32	96120.0
33	124397.0
34	167877.0
35	227456.0
36	336437.0
37	556011.0
38	1003439.0
39	1052497.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.08207781929386	18.05847224002494	12.120274548661044	24.73917539202016
2	17.64988363739796	21.831839335109475	35.47924466098326	25.039032366509307
3	20.516815832489854	24.736471401267806	29.171874232190127	25.574838534052212
4	14.412868707989018	15.25183383872827	33.60527506995926	36.730022383323444
5	14.326809002313212	37.845340568935256	32.758431966162675	15.069418462588857
6	34.06773548834463	33.861322194278095	16.462857697026674	15.6080846203506
7	28.256755361896662	31.26655706837483	22.06409654078983	18.41259102893868
8	27.780098992061447	33.31407606585985	20.714025158033962	18.191799784044736
9	28.227947460419685	13.586097535546429	20.04634744149175	38.13960756254214
10	17.630955702131498	26.522145294263094	32.07154031533212	23.775358688273286
11	35.21529356369601	22.001098756242254	21.36813092099225	21.415476759069485
12	21.871385199862615	26.236380271579474	29.778582157249023	22.113652371308888
13	29.922387665434186	19.20439232097826	26.438581580050997	24.434638433536556
14	22.723688284986064	20.918982457079995	26.534235252915433	29.823094005018504
15	25.911485382719988	26.716234630477565	23.520325560486583	23.851954426315864
16	23.628173191647683	25.76853787160048	26.48025943751272	24.123029499239113
17	23.47903770169106	26.454025527232698	26.239344261442625	23.827592509633615
18	23.647413125847113	24.24247309074203	28.378096946908443	23.73201683650242
19	23.984579972736494	25.20501579884597	28.319675146711	22.49072908170654
20	23.65500509988256	24.468984316073637	28.24097341587092	23.635037168172886
21	24.454112366935703	25.027098407323443	28.289775248968645	22.2290139767722
22	24.273490984660835	24.57342595888322	27.19473699399948	23.958346062456467
23	22.789000061619788	25.081022222903997	28.24367740662327	23.88630030885294
24	22.909405649832678	26.20310038539668	27.718375203156803	23.169118761613834
25	23.49622364291514	25.245809659330966	27.229992873424376	24.02797382432952
26	23.296622325551645	25.87836149600368	27.844136773052234	22.980879405392436
27	23.879670331527468	25.672520199980912	26.998073666588063	23.449735801903557
28	22.69105839658028	26.278734126729287	27.951490405902813	23.07871707078762
29	22.885147732794753	26.098190744187654	28.19794356303301	22.81871795998458
30	22.96436946185644	26.56018316417358	28.045220085347307	22.430227288622675
31	23.602719278700064	26.054276894373018	27.093649339719256	23.249354487207654
32	22.215988021320968	26.523705288927914	27.46141808195016	23.79888860780096
33	22.20317006515838	26.02078900890159	27.950164410437715	23.825876515502316
34	22.931505574250934	26.119328671895943	28.028424142789433	22.92074161106369
35	23.125412911087846	26.250836222140123	28.019168174444843	22.60458269232719
36	22.483449106604056	26.540605231130108	27.54680178993788	23.429143872327955
37	22.56963881183526	26.24826223094317	28.046364081434845	23.135734875786724
38	22.541688907423936	25.667580216875656	28.250411383593065	23.540319492107336
39	22.438027261946765	25.336497349179066	28.28803325492627	23.937442133947904
40	22.76255815205112	25.61402040005023	28.56992429085893	23.053497157039722
41	21.501224465812328	26.044370928251425	28.862241291134783	23.59216331480146
42	22.39575140653019	26.254710208891087	28.530092427083897	22.819445957494825
43	21.533386355818664	25.371051231004788	28.495330545969534	24.600231867207015
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	825.0
1	1155.5
2	1486.0
3	5166.5
4	8847.0
5	8847.0
6	12449.5
7	16052.0
8	15097.5
9	14143.0
10	19422.0
11	24701.0
12	24701.0
13	36590.5
14	48480.0
15	59435.0
16	70390.0
17	60126.5
18	49863.0
19	49863.0
20	51276.0
21	52689.0
22	42760.0
23	32831.0
24	37446.5
25	42062.0
26	42062.0
27	46977.5
28	51893.0
29	56252.0
30	60611.0
31	66507.0
32	72403.0
33	72403.0
34	84455.0
35	96507.0
36	113719.5
37	130932.0
38	150623.5
39	170315.0
40	170315.0
41	185442.5
42	200570.0
43	214976.5
44	229383.0
45	254705.5
46	280028.0
47	280028.0
48	312115.5
49	344203.0
50	346567.5
51	348932.0
52	323166.0
53	297400.0
54	297400.0
55	285110.0
56	272820.0
57	258864.5
58	244909.0
59	228077.5
60	211246.0
61	211246.0
62	178995.5
63	146745.0
64	128156.0
65	109567.0
66	93203.0
67	76839.0
68	76839.0
69	64069.5
70	51300.0
71	44113.0
72	36926.0
73	27932.0
74	18938.0
75	18938.0
76	15162.0
77	11386.0
78	9590.5
79	7795.0
80	6389.0
81	4983.0
82	4983.0
83	4200.5
84	3418.0
85	2845.5
86	2273.0
87	1542.5
88	812.0
89	812.0
90	597.5
91	383.0
92	265.0
93	147.0
94	132.0
95	117.0
96	117.0
97	63.0
98	9.0
99	8.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3846167.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.367624893378355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.27533904230825	40.61738075816201
2	9.178109122972856	9.062028968268356
3	3.0836619610623113	4.56699200975111
4	1.4883297904188744	2.9390122724415764
5	0.8874028356928729	2.1904485160903002
6	0.5806104827979424	1.7198016314359275
7	0.41429061731968625	1.43167806548791
8	0.30595198607198393	1.2083298307028656
9	0.22896091649459763	1.0172930976674477
>10	1.3658434116261684	12.269125827898995
>50	0.09937065233437148	3.372894362432191
>100	0.0735306527555058	7.502046021142335
>500	0.012102140859999755	4.098197867658912
>1k	0.006077265524478062	4.981343423997297
>5k	2.0956088015441592E-4	0.6134636455734032
>10k+	2.0956088015441592E-4	2.409963701289436
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	30741	0.7992632665196284	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23569	0.6127919042516874	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	21755	0.5656280655520157	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17114	0.44496247822832447	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6595	0.17146941357460557	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6267	0.1629414427402658	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	5796	0.1506954846214426	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	5061	0.13158554997741906	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4717	0.12264158056579447	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4375	0.11374961097633046	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3920	0.1019196514347921	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.5999911080304105E-5	0.0
4	0.0	0.0	0.0	5.199982216060821E-5	0.0
5	0.0	0.0	0.0	5.199982216060821E-5	0.0
6	0.0	0.0	0.0	5.199982216060821E-5	0.0
7	2.5999911080304105E-5	0.0	0.0	5.199982216060821E-5	2.5999911080304105E-5
8	5.199982216060821E-5	0.0	0.0	7.799973324091232E-5	2.5999911080304105E-5
9	5.199982216060821E-5	0.0	0.0	1.2999955540152054E-4	7.799973324091232E-5
10	5.199982216060821E-5	0.0	0.0	1.5599946648182464E-4	7.799973324091232E-5
11	5.199982216060821E-5	0.0	0.0	3.379988440439534E-4	7.799973324091232E-5
12	5.199982216060821E-5	0.0	0.0	6.239978659272986E-4	7.799973324091232E-5
13	5.199982216060821E-5	0.0	0.0	7.799973324091232E-4	7.799973324091232E-5
14	5.199982216060821E-5	0.0	0.0	0.0011179961764530765	1.5599946648182464E-4
15	5.199982216060821E-5	0.0	0.0	0.001507994842657638	1.5599946648182464E-4
16	5.199982216060821E-5	0.0	0.0	0.0036139876401622708	1.5599946648182464E-4
17	5.199982216060821E-5	0.0	0.0	0.0050699826606593005	1.5599946648182464E-4
18	5.199982216060821E-5	0.0	0.0	0.006343978303594202	1.5599946648182464E-4
19	5.199982216060821E-5	0.0	0.0	0.008709970211901875	1.5599946648182464E-4
20	5.199982216060821E-5	0.0	0.0	0.010919962653727725	1.5599946648182464E-4
21	5.199982216060821E-5	0.0	0.0	0.01627594433627037	1.5599946648182464E-4
22	5.199982216060821E-5	0.0	0.0	0.023763918727397953	1.5599946648182464E-4
23	5.199982216060821E-5	0.0	0.0	0.038401868665609164	2.0799928864243284E-4
24	5.199982216060821E-5	0.0	0.0	0.059435796729575184	2.0799928864243284E-4
25	5.199982216060821E-5	0.0	0.0	0.06952376222873317	2.3399919972273695E-4
26	5.199982216060821E-5	0.0	0.0	0.08452571092206865	2.3399919972273695E-4
27	5.199982216060821E-5	0.0	0.0	0.11611560288463814	2.3399919972273695E-4
28	5.199982216060821E-5	0.0	0.0	0.18920135293137297	2.3399919972273695E-4
29	5.199982216060821E-5	0.0	0.0	0.2773670514046842	2.3399919972273695E-4
30	5.199982216060821E-5	0.0	0.0	0.40627461054083197	2.3399919972273695E-4
31	5.199982216060821E-5	0.0	0.0	0.7248255210967178	2.599991108030411E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	845	0.0	18.171597	5
CAAGACG	875	0.0	17.971428	4
CTTATAC	3385	0.0	16.013294	37
GACGGAC	1005	0.0	15.094528	7
GTATTAG	890	0.0	14.966293	1
TCGTTAC	585	0.0	14.863248	23
ACGGACC	1030	0.0	14.728154	8
ACGTATG	80	0.0063021653	13.875	1
GTTACGG	965	0.0	13.803109	25
GGCGCAA	1060	0.0	13.787736	34
CGCAAGA	1130	0.0	13.752213	2
TCGTTTA	755	0.0	13.721855	30
CGTTACG	580	0.0	13.715517	24
CGCTTCG	770	0.0	13.694806	32
TCTAGCG	635	0.0	13.692913	28
CTAGCGG	640	0.0	13.5859375	29
CTAGACA	355	0.0	13.549295	4
GCGCAAG	1150	0.0	13.513043	1
TACTTAC	945	0.0	13.507936	31
ACGCTTC	850	0.0	13.494117	31
>>END_MODULE