FastQCFastQC Report
Thu 9 Feb 2017
SRR2727056.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727056.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2412049
Sequences flagged as poor quality0
Sequence length43
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT671512.7839815857803885No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT508172.1067979962264447No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT470201.9493799669907201No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT358661.4869515503209096No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131020.5431896284030714No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124840.5175682583562772No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA93260.38664222824660693No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA88810.36819318347181174No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87760.36384003807551174No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87480.36267919930316506No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT86850.36006731206538506No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC85600.35488499611740887No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG71350.2958065943104804No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA66570.2759894181254195No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA46600.19319673854055203No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA43210.17914229768964063No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG42620.17669624456219588No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG41240.17097496775563018No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40130.16637307119382733No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA40120.1663316126662435No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38980.16160534052168923No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA38490.15957387267008258No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA36700.15215279623258068No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT34410.14265879341588833No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA33580.13921773562643214No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA32090.1330404150164445No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA30380.1259510067996131No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA30070.12466579244451502No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29560.12255140753774073No Hit
GTACATGGGATTTATGGAAATGTAGGTAAAAAAAAAAAAAAAA28280.11724471600701313No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG27730.1149644969899036No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG27090.1123111512245398No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26890.1114819806728636No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC26650.11048697601085218No Hit
GATTTATGGAAATGTAGGTAAAAAAAAAAAAAAAAAAAAAAAA25810.1070044596938122No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT25180.10439257245603219No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA24800.10281714840784743No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24300.10074422202865697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT2100.020.2619041
CGTTAAT1156.413575E-817.6956521
CGTTACG1005.8831774E-616.65000224
CGTTATT2250.016.4444452
TATACCG803.384493E-416.18755
CGAACGA1455.353104E-815.31034416
AGTACTC7300.015.205485
GTTATTC2800.015.1964293
TAACCTC2600.014.9423078
CGCCGGG2600.014.94230718
TCGTTAC1252.961824E-614.79999923
GATCGCA2400.014.64583320
TCTAGCG1904.5656634E-1014.60526428
CGAGCCG3050.014.55737715
TCTAGAT2800.014.5357152
TCTACAC4350.014.459773
AAAAGTA6700.014.3582092
CCGATCG2353.6379788E-1214.17021418
AAGTACT7500.014.0600014
GGCCGAT2500.014.05999916
Produced by FastQC (version 0.11.5)