##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727055.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3697162
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18499595094832	33.0	31.0	34.0	30.0	34.0
2	32.34739835581995	34.0	31.0	34.0	30.0	34.0
3	32.45008171132344	34.0	31.0	34.0	30.0	34.0
4	36.008764019537146	37.0	35.0	37.0	35.0	37.0
5	35.80723944474167	37.0	35.0	37.0	35.0	37.0
6	35.84518395461167	37.0	35.0	37.0	35.0	37.0
7	35.80577778306712	37.0	35.0	37.0	35.0	37.0
8	35.81659094191707	37.0	35.0	37.0	35.0	37.0
9	37.5930527253066	39.0	37.0	39.0	35.0	39.0
10	37.45205295304885	39.0	37.0	39.0	35.0	39.0
11	37.518618605297796	39.0	37.0	39.0	35.0	39.0
12	37.449181831902415	39.0	37.0	39.0	35.0	39.0
13	37.477711011851795	39.0	37.0	39.0	35.0	39.0
14	38.699959861104276	40.0	38.0	41.0	35.0	41.0
15	38.60710918266497	40.0	38.0	41.0	34.0	41.0
16	38.56027217633417	40.0	38.0	41.0	34.0	41.0
17	38.61515427238514	40.0	38.0	41.0	34.0	41.0
18	38.62111451973162	40.0	38.0	41.0	35.0	41.0
19	38.68281914614507	40.0	38.0	41.0	34.0	41.0
20	38.65283344359809	40.0	38.0	41.0	34.0	41.0
21	38.55826090390413	40.0	38.0	41.0	34.0	41.0
22	38.51722780878955	40.0	38.0	41.0	34.0	41.0
23	38.424710358918546	40.0	38.0	41.0	34.0	41.0
24	38.34253976428406	40.0	38.0	41.0	34.0	41.0
25	38.299046403701	40.0	38.0	41.0	34.0	41.0
26	38.089879751009015	40.0	37.0	41.0	33.0	41.0
27	37.96890804352095	40.0	37.0	41.0	33.0	41.0
28	37.861666597244046	40.0	37.0	41.0	33.0	41.0
29	37.738216502279315	40.0	37.0	41.0	33.0	41.0
30	37.61682934099182	40.0	37.0	41.0	33.0	41.0
31	37.5034085604039	39.0	36.0	41.0	33.0	41.0
32	37.309498474776056	39.0	36.0	41.0	32.0	41.0
33	37.13291167657787	39.0	35.0	41.0	32.0	41.0
34	37.027204380008236	39.0	35.0	41.0	31.0	41.0
35	36.89287540010419	39.0	35.0	41.0	31.0	41.0
36	36.66848788340895	39.0	35.0	41.0	31.0	41.0
37	36.56820123110646	39.0	35.0	41.0	30.0	41.0
38	36.445079225633066	39.0	35.0	41.0	30.0	41.0
39	36.26473440979865	39.0	35.0	40.0	30.0	41.0
40	36.076733992181026	39.0	35.0	40.0	29.0	41.0
41	35.91744613841644	39.0	35.0	40.0	28.0	41.0
42	35.6139089928978	39.0	35.0	40.0	26.0	41.0
43	34.395063294494534	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	11.0
11	17.0
12	29.0
13	22.0
14	26.0
15	53.0
16	81.0
17	118.0
18	228.0
19	398.0
20	733.0
21	1365.0
22	2519.0
23	4345.0
24	7273.0
25	11329.0
26	17288.0
27	25603.0
28	35788.0
29	48088.0
30	61898.0
31	78246.0
32	98860.0
33	127749.0
34	169859.0
35	226649.0
36	332826.0
37	549969.0
38	945219.0
39	950569.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.658318461565926	18.061448213521615	12.14474778221782	24.13548554269464
2	17.693192778677265	22.09299998214847	35.3712928997972	24.842514339377068
3	20.729927441643074	24.945755690445807	29.35992526159254	24.964391606318575
4	14.360339092525564	15.308309454657385	33.436836146211604	36.89451530660545
5	14.120506485785583	38.437942400143676	32.49903574687828	14.942515367192458
6	33.86716622100952	33.801629466060724	16.528515656062677	15.802688656867078
7	28.1345799832412	31.66685690267291	21.79190416865693	18.40665894542895
8	28.411224609578916	33.01627031761119	20.44957727034953	18.122927802460374
9	28.490799158922435	13.33271303773002	20.031499836901926	38.14498796644561
10	17.75323883562581	27.09407918830714	31.81169772923123	23.340984246835816
11	34.92505873423994	22.227075794893487	21.21348753449267	21.634377936373898
12	21.9975213420456	26.771453347189006	29.643088401319716	21.587936909445677
13	30.20892782085286	19.269428821349997	26.496702064989307	24.024941292807835
14	22.825832354654732	21.106080826320298	26.93016968150165	29.137917137523324
15	25.943169382353275	26.758010603809083	23.95061401150396	23.348206002333683
16	23.23268496214123	25.710774913298362	27.00695831018495	24.049581814375458
17	23.153921845999715	26.619607147320025	26.580279684796064	23.646191321884192
18	23.118435167298593	24.199886291160624	28.967110448500772	23.71456809304001
19	23.550009439672916	25.087594214156695	29.273751055539353	22.088645290631028
20	23.423371764612966	24.49946742934175	29.07505811214115	23.002102693904135
21	24.1199330729895	24.84081032965285	29.098697866092966	21.940558731264684
22	23.929138079424163	24.70833033553845	27.98414026758903	23.378391317448358
23	22.622379003138082	25.231948180793808	28.940441343928125	23.20523147213998
24	22.46038988824401	26.43343732300613	28.3653786336655	22.74079415508436
25	23.04751590544315	25.54088784857142	28.02365706452679	23.38793918145864
26	22.994556365125465	26.016685230455145	28.50337637355355	22.485382030865836
27	23.164957337547015	25.98576962545866	27.840922307434727	23.00835072955959
28	22.299022872138146	26.48912868843724	28.74688747747597	22.464960961948652
29	22.462012754648025	26.363843402047298	28.839904770199414	22.334239073105262
30	22.447623339199094	27.02280830539749	28.697444147700317	21.832124207703096
31	23.072399856971373	26.545604439296955	27.818121034458322	22.56387466927335
32	21.709597794199983	27.044419476344288	28.22489249862462	23.021090230831106
33	21.772051102981152	26.583660656471096	28.63058205185491	23.01370618869284
34	22.34035186989372	26.655878211449757	28.633449115835337	22.37032080282119
35	22.445675899514274	26.89443957283992	28.619871133588408	22.040013394057386
36	21.894712755351268	27.149500076004244	28.117512838225643	22.838274330418844
37	22.009719887849112	26.9360390483295	28.4864174196316	22.567823644189787
38	21.901258316514127	26.48220445844678	28.707992779326414	22.908544445712682
39	21.929388000850384	26.098396553897285	28.735608555968064	23.236606889284268
40	22.094974469606687	26.384129232097486	29.02442468033589	22.496471617959937
41	21.000459271192337	26.806101544914725	29.240482294257053	22.95295688963589
42	21.75181936847777	26.965196548054966	28.912906710606677	22.37007737286059
43	21.057313690879653	26.195633299271172	28.85499742775675	23.892055582092425
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	460.0
1	788.0
2	1116.0
3	4661.0
4	8206.0
5	8206.0
6	11914.5
7	15623.0
8	14736.5
9	13850.0
10	19265.0
11	24680.0
12	24680.0
13	38206.0
14	51732.0
15	67307.0
16	82882.0
17	70844.0
18	58806.0
19	58806.0
20	60788.5
21	62771.0
22	49112.0
23	35453.0
24	40665.0
25	45877.0
26	45877.0
27	50847.5
28	55818.0
29	60147.0
30	64476.0
31	69887.0
32	75298.0
33	75298.0
34	85543.0
35	95788.0
36	111472.0
37	127156.0
38	144672.0
39	162188.0
40	162188.0
41	176886.5
42	191585.0
43	206128.0
44	220671.0
45	243087.0
46	265503.0
47	265503.0
48	294030.5
49	322558.0
50	322388.0
51	322218.0
52	300487.0
53	278756.0
54	278756.0
55	266985.0
56	255214.0
57	241675.5
58	228137.0
59	212166.0
60	196195.0
61	196195.0
62	167131.0
63	138067.0
64	119973.0
65	101879.0
66	86518.5
67	71158.0
68	71158.0
69	58624.5
70	46091.0
71	39011.0
72	31931.0
73	24685.5
74	17440.0
75	17440.0
76	13832.0
77	10224.0
78	8519.0
79	6814.0
80	5637.5
81	4461.0
82	4461.0
83	3683.0
84	2905.0
85	2408.0
86	1911.0
87	1292.5
88	674.0
89	674.0
90	502.5
91	331.0
92	232.0
93	133.0
94	119.0
95	105.0
96	105.0
97	58.5
98	12.0
99	10.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3697162.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.02961205011923
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.92230472056244	42.31493040192071
2	8.958507840137587	9.142983592603454
3	2.8841290667133617	4.415279621305658
4	1.4367977278169826	2.932769225799737
5	0.8450016027920936	2.1560051986104742
6	0.5675869426923118	1.737824489418116
7	0.400113798294709	1.4292356332015064
8	0.29551181143090416	1.2063882474837622
9	0.2281623161744409	1.0478731030954527
>10	1.2901770621551638	11.982666313283264
>50	0.08669963417609604	3.0235192850957664
>100	0.07013805624498565	7.46067191591262
>500	0.00991294721072304	3.519747234391145
>1k	0.004640102943105495	4.128897512721367
>5k	1.0545688507057941E-4	0.35046523364741483
>10k+	2.1091377014115882E-4	3.150742991509507
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	38874	1.0514551431611598	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	30795	0.832936181860573	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	27550	0.745166157176775	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19878	0.5376556396500883	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6583	0.17805549229381887	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6442	0.1742417562443842	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4977	0.13461676821302393	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4901	0.13256113743460524	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	4599	0.12439270986773097	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	4593	0.12423042322732951	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	4565	0.12347308557212261	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4402	0.11906429850788254	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	3839	0.10383640208354407	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.409554680049184E-5
2	0.0	0.0	0.0	0.0	5.409554680049184E-5
3	0.0	0.0	0.0	0.0	5.409554680049184E-5
4	0.0	0.0	0.0	5.409554680049184E-5	5.409554680049184E-5
5	0.0	0.0	0.0	5.409554680049184E-5	5.409554680049184E-5
6	0.0	0.0	0.0	5.409554680049184E-5	8.114332020073776E-5
7	0.0	0.0	0.0	5.409554680049184E-5	8.114332020073776E-5
8	0.0	0.0	0.0	5.409554680049184E-5	8.114332020073776E-5
9	0.0	0.0	0.0	5.409554680049184E-5	1.3523886700122958E-4
10	0.0	0.0	0.0	1.3523886700122958E-4	1.3523886700122958E-4
11	0.0	0.0	0.0	3.7866882760344284E-4	1.3523886700122958E-4
12	0.0	0.0	0.0	5.680032414051643E-4	1.3523886700122958E-4
13	0.0	0.0	0.0	6.220987882056561E-4	1.3523886700122958E-4
14	0.0	0.0	0.0	0.0011089587094100827	1.8933441380172142E-4
15	0.0	0.0	0.0	0.0017581052710159847	2.1638218720196735E-4
16	0.0	0.0	0.0	0.0035162105420319693	2.1638218720196735E-4
17	0.0	0.0	0.0	0.005328411359848446	2.1638218720196735E-4
18	2.704777340024592E-5	0.0	0.0	0.006302131202257299	2.1638218720196735E-4
19	2.704777340024592E-5	0.0	0.0	0.008709383034879186	2.1638218720196735E-4
20	2.704777340024592E-5	0.0	0.0	0.010386344985694433	2.1638218720196735E-4
21	2.704777340024592E-5	0.0	0.0	0.015092657557337222	2.4342996060221326E-4
22	2.704777340024592E-5	0.0	0.0	0.022287365281802637	2.4342996060221326E-4
23	2.704777340024592E-5	0.0	0.0	0.034999818779918215	2.975255074027051E-4
24	2.704777340024592E-5	0.0	0.0	0.05477174113549799	2.975255074027051E-4
25	2.704777340024592E-5	0.0	0.0	0.06459008287978725	3.24573280802951E-4
26	2.704777340024592E-5	0.0	0.0	0.07668043758969718	3.24573280802951E-4
27	2.704777340024592E-5	0.0	0.0	0.09896780287149981	3.24573280802951E-4
28	2.704777340024592E-5	0.0	0.0	0.14776198608554345	3.24573280802951E-4
29	2.704777340024592E-5	0.0	0.0	0.20888995397009924	3.24573280802951E-4
30	2.704777340024592E-5	0.0	0.0	0.3081282345756015	3.24573280802951E-4
31	2.704777340024592E-5	0.0	0.0	0.5723849806960041	3.24573280802951E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	740	0.0	15.75	5
ATACCGT	580	0.0	15.310345	6
CTTATAC	2345	0.0	15.147121	37
TCTAGCG	560	0.0	14.866072	28
CTAGCGG	580	0.0	14.672414	29
TCTTATA	3885	0.0	14.666667	37
CAAGACG	815	0.0	14.527607	4
TCGTTAC	385	0.0	14.4155855	23
TCTAACG	90	8.280134E-4	14.388888	2
CGCAATA	625	0.0	14.208	36
TACCGTC	535	0.0	13.831776	7
CATGGGT	2655	0.0	13.58757	4
GCGTTAT	600	0.0	13.566667	1
TAGCGGC	615	0.0	13.536585	30
CGATCGG	315	0.0	13.507937	36
ACGGACC	865	0.0	13.473988	8
TATACCG	110	2.4586712E-4	13.454544	5
ATTAGAC	155	1.8905448E-6	13.129032	3
GACGGAC	890	0.0	13.095505	7
GCGCAAG	910	0.0	13.010988	1
>>END_MODULE