FastQCFastQC Report
Thu 9 Feb 2017
SRR2727054.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727054.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4849568
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT230700.47571247583289894No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT193890.3998088077123571No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT173360.357475140053712No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127130.2621470613464952No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51300.10578261816310236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAC5750.016.08695623
CTTATAC37750.015.43708637
TAGATCG1101.4537069E-515.1363645
TATACCG2201.8189894E-1215.1363645
TCTAGCG8250.014.57575828
GTTACGG8650.013.90173325
GTATTAC3650.013.6849321
CAAGACG12100.013.6074394
CGTCGAT1102.4589174E-413.45454526
CTAGCGG8950.013.43575529
TCTTATA57200.013.13111837
CGTTACG5650.013.09734424
ATACCGT9050.013.0828726
GCGTTAT7750.012.8903221
AAGACGG12250.012.8367345
CGTATAC1307.006863E-512.8076933
GTCCTAA4400.012.6136361
CGCAATA9550.012.59162236
CGAGCCG11350.012.55066115
GTCCTAT11700.012.4914531
Produced by FastQC (version 0.11.5)