##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727054.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4849568
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22258188770628	33.0	31.0	34.0	30.0	34.0
2	32.40860587994642	34.0	31.0	34.0	31.0	34.0
3	32.523473431035505	34.0	31.0	34.0	31.0	34.0
4	36.06476886188626	37.0	35.0	37.0	35.0	37.0
5	35.86252899227313	37.0	35.0	37.0	35.0	37.0
6	35.884823761621654	37.0	35.0	37.0	35.0	37.0
7	35.85335745369485	37.0	35.0	37.0	35.0	37.0
8	35.847368466634556	37.0	35.0	37.0	35.0	37.0
9	37.641849954470175	39.0	37.0	39.0	35.0	39.0
10	37.50054499699767	39.0	37.0	39.0	35.0	39.0
11	37.570657221426735	39.0	37.0	39.0	35.0	39.0
12	37.48907284112729	39.0	37.0	39.0	35.0	39.0
13	37.515251461573484	39.0	37.0	39.0	35.0	39.0
14	38.78852363756937	40.0	38.0	41.0	35.0	41.0
15	38.700870675491096	40.0	38.0	41.0	35.0	41.0
16	38.67008710878989	40.0	38.0	41.0	35.0	41.0
17	38.67646705850913	40.0	38.0	41.0	35.0	41.0
18	38.669347661482426	40.0	38.0	41.0	35.0	41.0
19	38.722870573213946	40.0	38.0	41.0	35.0	41.0
20	38.674817014628935	40.0	38.0	41.0	34.0	41.0
21	38.58321256656263	40.0	38.0	41.0	34.0	41.0
22	38.5423594431504	40.0	38.0	41.0	34.0	41.0
23	38.46743565612442	40.0	38.0	41.0	34.0	41.0
24	38.39174499666775	40.0	38.0	41.0	34.0	41.0
25	38.35425279117645	40.0	38.0	41.0	34.0	41.0
26	38.17082284442655	40.0	38.0	41.0	34.0	41.0
27	38.07268008201968	40.0	38.0	41.0	33.0	41.0
28	37.97478414572185	40.0	37.0	41.0	33.0	41.0
29	37.87388629255224	40.0	37.0	41.0	33.0	41.0
30	37.80083957993784	40.0	37.0	41.0	33.0	41.0
31	37.7353250846261	40.0	37.0	41.0	33.0	41.0
32	37.60558198173528	40.0	37.0	41.0	33.0	41.0
33	37.46304763640803	40.0	37.0	41.0	32.0	41.0
34	37.390065671828914	40.0	36.0	41.0	32.0	41.0
35	37.29926892457225	40.0	36.0	41.0	32.0	41.0
36	37.12260535371398	39.0	36.0	41.0	31.0	41.0
37	37.07184062580419	39.0	36.0	41.0	31.0	41.0
38	36.99791486581898	39.0	36.0	41.0	31.0	41.0
39	36.87025297923444	39.0	36.0	41.0	31.0	41.0
40	36.74197742974219	39.0	35.0	41.0	30.0	41.0
41	36.64846415185848	39.0	35.0	41.0	30.0	41.0
42	36.420935019366674	39.0	35.0	41.0	30.0	41.0
43	35.18309465915315	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	9.0
10	8.0
11	24.0
12	22.0
13	21.0
14	23.0
15	47.0
16	88.0
17	146.0
18	219.0
19	442.0
20	859.0
21	1575.0
22	2921.0
23	4792.0
24	7961.0
25	12612.0
26	18965.0
27	27691.0
28	39520.0
29	53602.0
30	71312.0
31	91125.0
32	117992.0
33	152866.0
34	206812.0
35	283118.0
36	413473.0
37	677040.0
38	1284021.0
39	1380260.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.22402160357376	18.11082966565269	12.068580129199137	25.59656860157441
2	17.85561105648998	20.904026915387103	36.044983800618944	25.19537822750398
3	19.968294083101835	23.787314663904084	29.29609400259982	26.94829725039426
4	14.202419679443611	15.05824848728794	34.4612551056094	36.27807672765904
5	14.614023352183123	37.14730879121604	33.33253601145504	14.906131845145795
6	34.48311272261776	34.50416614428337	16.172162138978152	14.840558994120714
7	28.88319124507585	30.88466849005932	21.757010108941664	18.475130155923168
8	27.2663049574725	34.11211060449096	20.242483454196332	18.37910098384021
9	27.849490923727636	13.929426291166552	19.598694151726505	38.6223886333793
10	17.29737576625382	26.155731809513753	32.592964981623105	23.95392744260932
11	36.134331965238964	21.589840579614513	21.43467211924856	20.84115533589796
12	21.807839378682804	25.372796092352967	29.982773723350203	22.836590805614026
13	30.093051587275404	19.3380111383117	25.76470728939155	24.804229985021344
14	22.751263617707803	20.415735999577695	25.90302888834634	30.929971494368157
15	25.564338926683778	27.093526681139434	22.790627948716256	24.55150644346053
16	24.536185491161273	25.69055635471036	25.199069277923314	24.574188876205056
17	23.86130063543804	26.170557872371315	25.588526648146804	24.37961484404384
18	24.05898834700328	24.584643415660942	27.13165378854364	24.224714448792138
19	24.530597364548758	25.05674732264812	27.022551287042475	23.390104025760643
20	24.25444905608087	24.42137114068717	26.95623610185485	24.36794370137711
21	25.004474625368694	24.836067872437297	26.978918534599373	23.18053896759464
22	24.82544836983418	24.360066711096742	26.50304109561924	24.311443823449842
23	23.729556941979162	24.764762552045873	27.028531201129667	24.477149304845298
24	23.74314578123247	25.451937162237957	26.750073408600517	24.054843647929054
25	24.26884209067694	24.86972035447281	26.390659951566818	24.470777603283427
26	23.979950379085313	25.519139024341964	26.718586067872437	23.78232452870029
27	24.60433589136187	25.287448284053344	26.114037374050636	23.99417845053415
28	23.66342734033217	25.55644131601	26.74271192815525	24.037419415502576
29	23.58698754198312	25.75899544041861	26.97700083801279	23.67701617958548
30	23.882663363004706	25.78986416934457	26.839895842268835	23.48757662538189
31	24.155759853248785	25.370734053012555	26.39511395654211	24.07839213719655
32	23.3963932457489	25.644511016238976	26.47594589868623	24.483149839325893
33	23.23588410349128	25.182820407920868	26.888601211489355	24.692694277098497
34	24.06247319348857	25.26233264488713	27.085299144171195	23.5898950174531
35	24.153697813908373	25.24156790872919	27.000941114754962	23.603793162607474
36	23.322695959722598	25.60378573926585	26.868888115395023	24.204630185616534
37	23.619196596480347	25.08345073210645	27.19215402279131	24.105198648621897
38	23.33348042547295	24.69632346633762	27.527482860329002	24.44271324786043
39	23.360225075718084	24.549629987660758	27.65068971091858	24.439455225702577
40	23.591379685778197	24.60975905482715	27.978203419356117	23.820657840038535
41	22.484291384304747	25.031796646629147	28.296582293515627	24.18732967555048
42	23.128080686774574	25.122938785475323	28.024784888056008	23.72419563969409
43	22.37145659159744	24.435207424661332	28.015938739285644	25.17739724445559
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1026.0
1	1257.5
2	1489.0
3	4479.5
4	7470.0
5	7470.0
6	9822.0
7	12174.0
8	11232.5
9	10291.0
10	14183.0
11	18075.0
12	18075.0
13	27062.5
14	36050.0
15	44297.5
16	52545.0
17	46716.5
18	40888.0
19	40888.0
20	43688.0
21	46488.0
22	40420.5
23	34353.0
24	40406.0
25	46459.0
26	46459.0
27	54100.5
28	61742.0
29	69153.0
30	76564.0
31	86861.0
32	97158.0
33	97158.0
34	113709.5
35	130261.0
36	152602.5
37	174944.0
38	200171.5
39	225399.0
40	225399.0
41	246469.0
42	267539.0
43	286953.0
44	306367.0
45	333911.5
46	361456.0
47	361456.0
48	389096.5
49	416737.0
50	421354.0
51	425971.0
52	413286.5
53	400602.0
54	400602.0
55	385484.5
56	370367.0
57	350376.0
58	330385.0
59	305589.5
60	280794.0
61	280794.0
62	239299.0
63	197804.0
64	172040.0
65	146276.0
66	124050.0
67	101824.0
68	101824.0
69	83419.5
70	65015.0
71	55078.5
72	45142.0
73	34204.5
74	23267.0
75	23267.0
76	18480.5
77	13694.0
78	11494.5
79	9295.0
80	7493.0
81	5691.0
82	5691.0
83	4765.0
84	3839.0
85	3171.5
86	2504.0
87	1688.5
88	873.0
89	873.0
90	659.5
91	446.0
92	298.5
93	151.0
94	142.0
95	133.0
96	133.0
97	71.5
98	10.0
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4849568.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.94454747548096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.5990244926902	42.90568949188961
2	8.932030447901697	9.279405593069425
3	2.969139027444844	4.626917495172363
4	1.4842015626940785	3.0838471414618245
5	0.8606907940569654	2.235409690680073
6	0.5948021510122692	1.8538037143065007
7	0.42004989392892295	1.5273511160083177
8	0.2943373460806191	1.2231376197833432
9	0.24486689292951067	1.1447549950455433
>10	1.4263200013032127	13.594204045471797
>50	0.0999803594166324	3.542269099355564
>100	0.060710001554787764	6.1570576232066765
>500	0.009139040053508424	3.296223544819691
>1k	0.004510174271949095	3.9344068981445455
>5k	3.9562932210060655E-5	0.1054255327163277
>10k+	1.5825172884024262E-4	1.4900963988685163
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	23070	0.47571247583289894	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	19389	0.3998088077123571	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	17336	0.357475140053712	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12713	0.2621470613464952	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5130	0.10578261816310236	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.062039340411352E-5
2	0.0	0.0	0.0	0.0	2.062039340411352E-5
3	0.0	0.0	0.0	0.0	2.062039340411352E-5
4	0.0	0.0	0.0	2.062039340411352E-5	2.062039340411352E-5
5	0.0	0.0	0.0	2.062039340411352E-5	2.062039340411352E-5
6	0.0	0.0	0.0	2.062039340411352E-5	4.124078680822704E-5
7	0.0	0.0	0.0	2.062039340411352E-5	4.124078680822704E-5
8	0.0	0.0	0.0	2.062039340411352E-5	1.4434275382879466E-4
9	0.0	0.0	0.0	4.124078680822704E-5	1.6496314723290817E-4
10	0.0	0.0	0.0	1.031019670205676E-4	1.6496314723290817E-4
11	0.0	0.0	0.0	1.2372236042468111E-4	1.6496314723290817E-4
12	0.0	0.0	0.0	1.6496314723290817E-4	1.6496314723290817E-4
13	0.0	0.0	0.0	2.062039340411352E-4	1.6496314723290817E-4
14	0.0	0.0	0.0	4.124078680822704E-4	2.062039340411352E-4
15	0.0	0.0	0.0	8.866769163768814E-4	2.4744472084936223E-4
16	2.062039340411352E-5	0.0	0.0	0.0019176965865825575	2.886855076575893E-4
17	2.062039340411352E-5	0.0	0.0	0.002845614289767666	2.886855076575893E-4
18	2.062039340411352E-5	0.0	0.0	0.0033405037314663904	3.2992629446581634E-4
19	2.062039340411352E-5	0.0	0.0	0.004474625368692634	3.2992629446581634E-4
20	2.062039340411352E-5	0.0	0.0	0.005608747005918878	3.2992629446581634E-4
21	2.062039340411352E-5	0.0	0.0	0.007918231067179591	3.2992629446581634E-4
22	2.062039340411352E-5	0.0	0.0	0.011341216372262437	3.2992629446581634E-4
23	2.062039340411352E-5	0.0	0.0	0.018475872490085716	3.505466878699299E-4
24	2.062039340411352E-5	0.0	0.0	0.028579865258101342	3.7116708127404337E-4
25	2.062039340411352E-5	0.0	0.0	0.03326069456083511	3.917874746781569E-4
26	2.062039340411352E-5	0.0	0.0	0.040147905957809024	4.124078680822704E-4
27	2.062039340411352E-5	0.0	0.0	0.06509858197678639	4.3302826148638394E-4
28	2.062039340411352E-5	0.0	0.0	0.1279289206791203	4.536486548904975E-4
29	2.062039340411352E-5	0.0	0.0	0.2119982645876911	4.536486548904975E-4
30	2.062039340411352E-5	0.0	0.0	0.3266682723079664	4.536486548904975E-4
31	2.062039340411352E-5	0.0	0.0	0.6391497139539027	4.536486548904975E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAC	575	0.0	16.086956	23
CTTATAC	3775	0.0	15.437086	37
TAGATCG	110	1.4537069E-5	15.136364	5
TATACCG	220	1.8189894E-12	15.136364	5
TCTAGCG	825	0.0	14.575758	28
GTTACGG	865	0.0	13.901733	25
GTATTAC	365	0.0	13.684932	1
CAAGACG	1210	0.0	13.607439	4
CGTCGAT	110	2.4589174E-4	13.454545	26
CTAGCGG	895	0.0	13.435755	29
TCTTATA	5720	0.0	13.131118	37
CGTTACG	565	0.0	13.097344	24
ATACCGT	905	0.0	13.082872	6
GCGTTAT	775	0.0	12.890322	1
AAGACGG	1225	0.0	12.836734	5
CGTATAC	130	7.006863E-5	12.807693	3
GTCCTAA	440	0.0	12.613636	1
CGCAATA	955	0.0	12.591622	36
CGAGCCG	1135	0.0	12.550661	15
GTCCTAT	1170	0.0	12.491453	1
>>END_MODULE