FastQCFastQC Report
Thu 9 Feb 2017
SRR2727053.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727053.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3748396
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT453791.2106244911156665No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT346540.9245021070345822No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT318210.8489231127127443No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239770.6396602706864483No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC92630.24711903438163949No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89290.23820855640652697No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83890.22380239441083602No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG78230.20870260239313027No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62590.1669780887611661No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60670.16185589782936488No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT58140.15510634415360597No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA55330.14760980430029272No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA51610.13768555936992782No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA50540.13483100504855944No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA38090.10161679822516086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG4850.018.69072228
CTAGCGG5000.018.1329
TCGTTAC3200.016.76562523
CAAGACG8250.016.1454544
AAGACGG7850.016.0254785
TAGCGGC6000.015.72499930
TATACTG3850.015.3766235
GTTACGG5150.015.08737825
CGCAATA6200.014.91935536
GCGCAAT6400.014.74218835
ATACCGT6450.014.3410856
CCGTTAA1051.6572075E-414.09523936
CGTTACG3300.014.01515124
AATACCG800.006302125313.8750015
CTTATAC37500.013.71466637
TACGGAT7050.013.645390527
ACGGATG6700.013.52985128
ACGGACC8700.013.3965528
GGCGCAA9550.013.36649334
CATGGGT30300.013.3102314
Produced by FastQC (version 0.11.5)