##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727053.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3748396
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.120241831439365	33.0	31.0	34.0	30.0	34.0
2	32.26736582794347	33.0	31.0	34.0	30.0	34.0
3	32.37238354752273	34.0	31.0	34.0	30.0	34.0
4	35.939812389085894	37.0	35.0	37.0	35.0	37.0
5	35.7375904253446	37.0	35.0	37.0	35.0	37.0
6	35.790279095378395	37.0	35.0	37.0	35.0	37.0
7	35.74643154031751	37.0	35.0	37.0	35.0	37.0
8	35.76830542984253	37.0	35.0	37.0	35.0	37.0
9	37.525137952340145	39.0	37.0	39.0	35.0	39.0
10	37.387808812089226	39.0	37.0	39.0	34.0	39.0
11	37.451024384830205	39.0	37.0	39.0	35.0	39.0
12	37.388293285981526	39.0	37.0	39.0	34.0	39.0
13	37.419282274338144	39.0	37.0	39.0	35.0	39.0
14	38.61264845016375	40.0	38.0	41.0	34.0	41.0
15	38.51597136481845	40.0	38.0	41.0	34.0	41.0
16	38.456339991825836	40.0	38.0	41.0	34.0	41.0
17	38.53323394860095	40.0	38.0	41.0	34.0	41.0
18	38.5453796770672	40.0	38.0	41.0	34.0	41.0
19	38.6018208855201	40.0	38.0	41.0	34.0	41.0
20	38.57557392548706	40.0	38.0	41.0	34.0	41.0
21	38.46933648419217	40.0	38.0	41.0	34.0	41.0
22	38.426255657086394	40.0	38.0	41.0	34.0	41.0
23	38.32140654295864	40.0	38.0	41.0	34.0	41.0
24	38.22741594004476	40.0	38.0	41.0	34.0	41.0
25	38.186261270153956	40.0	37.0	41.0	34.0	41.0
26	37.965619961178064	40.0	37.0	41.0	33.0	41.0
27	37.83424563466613	40.0	37.0	41.0	33.0	41.0
28	37.71779929335108	40.0	37.0	41.0	33.0	41.0
29	37.57998354496163	40.0	37.0	41.0	33.0	41.0
30	37.438686307423225	39.0	36.0	41.0	33.0	41.0
31	37.298192613587254	39.0	36.0	41.0	32.0	41.0
32	37.073648568614416	39.0	35.0	41.0	32.0	41.0
33	36.88191935964076	39.0	35.0	41.0	31.0	41.0
34	36.74956888226324	39.0	35.0	41.0	31.0	41.0
35	36.5957991098059	39.0	35.0	40.0	31.0	41.0
36	36.34588581355865	39.0	35.0	40.0	30.0	41.0
37	36.2130775403666	39.0	35.0	40.0	30.0	41.0
38	36.07141961521675	39.0	35.0	40.0	30.0	41.0
39	35.86031011664723	39.0	35.0	40.0	28.0	41.0
40	35.64623961822603	39.0	35.0	40.0	27.0	41.0
41	35.46158036664216	38.0	35.0	40.0	26.0	41.0
42	35.12715412138952	38.0	35.0	40.0	24.0	41.0
43	33.937287842586535	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	13.0
10	40.0
11	38.0
12	52.0
13	30.0
14	36.0
15	45.0
16	80.0
17	129.0
18	226.0
19	444.0
20	893.0
21	1641.0
22	2996.0
23	5139.0
24	8442.0
25	13490.0
26	20416.0
27	30063.0
28	41342.0
29	55038.0
30	69637.0
31	85618.0
32	107271.0
33	136218.0
34	178830.0
35	237983.0
36	351133.0
37	578093.0
38	962697.0
39	860317.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.98076083743553	18.295852412605285	12.432971329603381	23.2904154203558
2	17.24233512147596	22.948562531813607	35.29122856816622	24.517873778544207
3	21.064690070099317	26.05773242741695	29.22799512111314	23.64958238137059
4	14.363957276659137	15.724379174452219	33.15279388837252	36.758869660516126
5	13.757217753940617	39.041552706811125	32.30645854920345	14.894770990044808
6	32.984802032656106	33.49518033847011	17.17081119497513	16.34920643389866
7	27.29223379813659	32.02668554763157	22.383520844649286	18.297559809582552
8	28.47185836288375	32.562594773871275	21.15795662998253	17.80759023326244
9	28.475513259538214	13.455702118986363	20.72358416773468	37.345200453740745
10	18.019947732310033	27.444085416802285	31.58652394250768	22.94944290838001
11	33.72594571117886	22.773581019721505	21.492953252537887	22.007520016561752
12	21.79073395660437	27.688830102262408	29.81160475040524	20.70883119072798
13	29.853435976348287	19.47993221634	27.305839617799187	23.360792189512527
14	22.660919497299645	21.658277300477323	27.694352464360755	27.986450737862274
15	25.869892081839808	26.625975483913656	24.84518711470186	22.658945319544678
16	22.30001846122982	26.007630997365272	28.221084431847647	23.471266109557263
17	22.647527102259208	27.201288231019348	27.3559143697731	22.79527029694835
18	22.63021302978661	24.139205142679696	30.12179076063468	23.108791066899016
19	22.753465749083073	25.775131549601483	30.296158676938084	21.17524402437736
20	22.773367595099344	24.780759556887798	30.301974497891898	22.143898350120956
21	23.457793680283515	25.35548538628256	30.117895761280288	21.068825172153634
22	23.33115284511028	25.238315268717603	28.602340841255835	22.82819104491628
23	21.70640455277404	25.861888658508864	29.796184821454297	22.635521967262797
24	21.575441869002102	27.423089769597446	29.236478749843936	21.76498961155652
25	22.320934074201336	26.03548291055694	28.75187146715555	22.891711548086167
26	22.235510869182445	26.573473026862686	29.51753229914876	21.67348380480611
27	22.53718657260332	26.552850872746635	28.568966565965816	22.340995988684227
28	21.487884417761624	27.1387014605714	29.824383549656975	21.54903057201
29	21.787959436516314	26.773638644369484	29.951131097141282	21.487270821972917
30	21.496501436881267	27.878217776350205	29.82371659771273	20.8015641890558
31	22.387175741303746	27.06197530890546	28.585213515327624	21.965635434463167
32	20.61489767890052	27.82166025147823	29.228635394979612	22.334806674641634
33	20.791826690669822	27.398039054571612	29.58748221906117	22.2226520356974
34	21.239084664480487	27.368506422480444	29.56037729204705	21.83203162099202
35	21.322960540988735	27.727593349261927	29.715723738900586	21.233722370848756
36	21.041613532828443	27.756058858242298	28.974926875388835	22.227400733540424
37	21.018857132490805	27.621948161293524	29.432562621451947	21.92663208476372
38	20.971716969071572	27.145637760791548	29.681442408966397	22.201202861170486
39	21.08536558037091	26.536763991851448	29.578651775319365	22.79921865245828
40	21.064903494721477	27.069018321436687	29.91428867174119	21.951789512100643
41	19.974036894714434	27.420848811064786	30.101115250363087	22.503999043857693
42	20.794467820369032	27.69611321749356	29.737092879194194	21.77232608294321
43	20.064795715287286	26.652680239761224	29.770573866795292	23.511950178156205
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	890.0
1	1320.5
2	1751.0
3	6611.5
4	11472.0
5	11472.0
6	16673.5
7	21875.0
8	21127.0
9	20379.0
10	27754.0
11	35129.0
12	35129.0
13	51812.5
14	68496.0
15	85636.5
16	102777.0
17	87824.5
18	72872.0
19	72872.0
20	73447.5
21	74023.0
22	58124.0
23	42225.0
24	47630.5
25	53036.0
26	53036.0
27	57492.0
28	61948.0
29	65062.0
30	68176.0
31	72423.0
32	76670.0
33	76670.0
34	86159.0
35	95648.0
36	110454.5
37	125261.0
38	142145.0
39	159029.0
40	159029.0
41	174016.5
42	189004.0
43	204289.5
44	219575.0
45	244545.0
46	269515.0
47	269515.0
48	307395.0
49	345275.0
50	342419.0
51	339563.0
52	302547.5
53	265532.0
54	265532.0
55	251941.5
56	238351.0
57	225289.0
58	212227.0
59	196701.5
60	181176.0
61	181176.0
62	154154.0
63	127132.0
64	110688.0
65	94244.0
66	79755.5
67	65267.0
68	65267.0
69	53496.5
70	41726.0
71	35368.5
72	29011.0
73	21955.5
74	14900.0
75	14900.0
76	11840.0
77	8780.0
78	7460.5
79	6141.0
80	4997.0
81	3853.0
82	3853.0
83	3168.5
84	2484.0
85	2141.0
86	1798.0
87	1212.0
88	626.0
89	626.0
90	471.5
91	317.0
92	221.0
93	125.0
94	111.5
95	98.0
96	98.0
97	55.0
98	12.0
99	9.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3748396.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.194852618356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.22779651508105	40.11151386794855
2	8.687305061200613	8.373667741505235
3	2.836362038417563	4.100941512415028
4	1.4005616382959485	2.6999944696238587
5	0.8425263812968206	2.030271738683854
6	0.5365796331601299	1.5516225802898396
7	0.38113723318540416	1.2858196946516975
8	0.28230610560803077	1.0884560922432873
9	0.23368632514160206	1.013623019921226
>10	1.3663942962465367	12.13191087206565
>50	0.10637899282824363	3.570515634241303
>100	0.08006552008182587	8.0140088076971
>500	0.01241703092568362	4.081048896879781
>1k	0.005714031925273231	4.541527610587046
>5k	5.494261466608876E-4	1.8083388446350996
>10k+	2.1977045866435502E-4	3.596738616611465
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	45379	1.2106244911156665	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	34654	0.9245021070345822	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	31821	0.8489231127127443	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23977	0.6396602706864483	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	9263	0.24711903438163949	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8929	0.23820855640652697	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8389	0.22380239441083602	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	7823	0.20870260239313027	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6259	0.1669780887611661	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6067	0.16185589782936488	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5814	0.15510634415360597	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	5533	0.14760980430029272	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	5161	0.13768555936992782	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	5054	0.13483100504855944	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA	3809	0.10161679822516086	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.003423330939421E-5
2	0.0	0.0	0.0	0.0	8.003423330939421E-5
3	0.0	0.0	0.0	0.0	8.003423330939421E-5
4	0.0	0.0	0.0	2.667807776979807E-5	8.003423330939421E-5
5	0.0	2.667807776979807E-5	0.0	5.335615553959614E-5	8.003423330939421E-5
6	5.335615553959614E-5	2.667807776979807E-5	0.0	5.335615553959614E-5	8.003423330939421E-5
7	5.335615553959614E-5	2.667807776979807E-5	0.0	5.335615553959614E-5	8.003423330939421E-5
8	5.335615553959614E-5	2.667807776979807E-5	0.0	5.335615553959614E-5	8.003423330939421E-5
9	5.335615553959614E-5	2.667807776979807E-5	0.0	1.3339038884899033E-4	8.003423330939421E-5
10	5.335615553959614E-5	2.667807776979807E-5	0.0	2.4010269992818262E-4	8.003423330939421E-5
11	5.335615553959614E-5	2.667807776979807E-5	0.0	5.068834776261633E-4	8.003423330939421E-5
12	5.335615553959614E-5	2.667807776979807E-5	0.0	9.870888774825286E-4	8.003423330939421E-5
13	5.335615553959614E-5	5.335615553959614E-5	0.0	0.0012005134996409131	8.003423330939421E-5
14	5.335615553959614E-5	5.335615553959614E-5	0.0	0.0021075681438140472	1.0671231107919228E-4
15	5.335615553959614E-5	5.335615553959614E-5	0.0	0.0032814035656851624	1.0671231107919228E-4
16	5.335615553959614E-5	5.335615553959614E-5	0.0	0.006616163286909921	1.3339038884899033E-4
17	5.335615553959614E-5	5.335615553959614E-5	0.0	0.01064455303014943	1.3339038884899033E-4
18	5.335615553959614E-5	5.335615553959614E-5	0.0	0.013045580029431255	2.1342462215838455E-4
19	5.335615553959614E-5	5.335615553959614E-5	0.0	0.01728739439482915	2.4010269992818262E-4
20	5.335615553959614E-5	5.335615553959614E-5	0.0	0.020968969127061282	2.4010269992818262E-4
21	5.335615553959614E-5	5.335615553959614E-5	0.0	0.03134674137951273	2.4010269992818262E-4
22	5.335615553959614E-5	5.335615553959614E-5	0.0	0.045806259530743285	2.6678077769798066E-4
23	5.335615553959614E-5	5.335615553959614E-5	0.0	0.07147057034528903	3.73493088777173E-4
24	5.335615553959614E-5	5.335615553959614E-5	0.0	0.10996703656710764	3.73493088777173E-4
25	5.335615553959614E-5	5.335615553959614E-5	0.0	0.12656080093992203	3.73493088777173E-4
26	5.335615553959614E-5	5.335615553959614E-5	0.0	0.15147812557691342	3.73493088777173E-4
27	5.335615553959614E-5	5.335615553959614E-5	0.0	0.19320263920887762	3.73493088777173E-4
28	5.335615553959614E-5	5.335615553959614E-5	0.0	0.2831077612930971	3.73493088777173E-4
29	5.335615553959614E-5	5.335615553959614E-5	0.0	0.38501801837372573	3.73493088777173E-4
30	5.335615553959614E-5	5.335615553959614E-5	0.0	0.5441527522705712	3.73493088777173E-4
31	5.335615553959614E-5	5.335615553959614E-5	0.0	0.9117233077828489	3.73493088777173E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	485	0.0	18.690722	28
CTAGCGG	500	0.0	18.13	29
TCGTTAC	320	0.0	16.765625	23
CAAGACG	825	0.0	16.145454	4
AAGACGG	785	0.0	16.025478	5
TAGCGGC	600	0.0	15.724999	30
TATACTG	385	0.0	15.376623	5
GTTACGG	515	0.0	15.087378	25
CGCAATA	620	0.0	14.919355	36
GCGCAAT	640	0.0	14.742188	35
ATACCGT	645	0.0	14.341085	6
CCGTTAA	105	1.6572075E-4	14.095239	36
CGTTACG	330	0.0	14.015151	24
AATACCG	80	0.0063021253	13.875001	5
CTTATAC	3750	0.0	13.714666	37
TACGGAT	705	0.0	13.6453905	27
ACGGATG	670	0.0	13.529851	28
ACGGACC	870	0.0	13.396552	8
GGCGCAA	955	0.0	13.366493	34
CATGGGT	3030	0.0	13.310231	4
>>END_MODULE