FastQCFastQC Report
Thu 9 Feb 2017
SRR2727052.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727052.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4610622
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT361150.7832999538890849No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT286400.6211743231173582No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT263670.5718751179341963No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187110.40582376954779636No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66540.14431892269633034No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64900.14076191889077005No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49170.10664504702402408No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47810.10369533655112044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG8000.014.5687529
CTTATAC42000.014.53571437
GGACCGT2201.8189894E-1114.2954546
TCTAGCG8100.013.93209828
GTCCTAT9550.013.5602091
TCTTATA65300.013.513782537
ATACCGT7850.013.4331216
AAGACGG9250.013.45
GGGTACG1254.8505688E-513.321
GCGCAAT8700.012.97126535
CCTATTC10700.012.9672893
TACCGTA1000.001833935312.9500017
TAGCGGC8900.012.8876430
CGCAATA8950.012.81564236
CGGCGCA12950.012.71428633
TACCGTC7600.012.6578957
GTACTAA2052.0701918E-812.6341461
GCCGGTC10450.012.5693788
CATGGGT31350.012.3923444
TACTATA5400.012.3333342
Produced by FastQC (version 0.11.5)