##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727052.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4610622
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.201978388165415	33.0	31.0	34.0	30.0	34.0
2	32.37423584063061	34.0	31.0	34.0	30.0	34.0
3	32.48217507312462	34.0	31.0	34.0	30.0	34.0
4	36.033452319448436	37.0	35.0	37.0	35.0	37.0
5	35.8319998906872	37.0	35.0	37.0	35.0	37.0
6	35.868716194908195	37.0	35.0	37.0	35.0	37.0
7	35.828267639377074	37.0	35.0	37.0	35.0	37.0
8	35.83431020803701	37.0	35.0	37.0	35.0	37.0
9	37.6188355931152	39.0	37.0	39.0	35.0	39.0
10	37.47886857781879	39.0	37.0	39.0	35.0	39.0
11	37.54420032698408	39.0	37.0	39.0	35.0	39.0
12	37.47209248556919	39.0	37.0	39.0	35.0	39.0
13	37.50065717814213	39.0	37.0	39.0	35.0	39.0
14	38.744501934879935	40.0	38.0	41.0	35.0	41.0
15	38.65330014041489	40.0	38.0	41.0	35.0	41.0
16	38.61473441110549	40.0	38.0	41.0	34.0	41.0
17	38.647231111116895	40.0	38.0	41.0	35.0	41.0
18	38.64840492237273	40.0	38.0	41.0	35.0	41.0
19	38.70356320687317	40.0	38.0	41.0	35.0	41.0
20	38.66367748212714	40.0	38.0	41.0	34.0	41.0
21	38.565254319265385	40.0	38.0	41.0	34.0	41.0
22	38.52195322019458	40.0	38.0	41.0	34.0	41.0
23	38.43627584304244	40.0	38.0	41.0	34.0	41.0
24	38.35546115036106	40.0	38.0	41.0	34.0	41.0
25	38.31183167910967	40.0	38.0	41.0	34.0	41.0
26	38.11493286589098	40.0	38.0	41.0	34.0	41.0
27	37.99851213133499	40.0	37.0	41.0	33.0	41.0
28	37.89583899959702	40.0	37.0	41.0	33.0	41.0
29	37.779302228636396	40.0	37.0	41.0	33.0	41.0
30	37.68063918490824	40.0	37.0	41.0	33.0	41.0
31	37.58645861664652	40.0	37.0	41.0	33.0	41.0
32	37.42181792391569	40.0	36.0	41.0	32.0	41.0
33	37.255200491387065	39.0	36.0	41.0	32.0	41.0
34	37.16179292078162	39.0	36.0	41.0	31.0	41.0
35	37.04433436529822	39.0	36.0	41.0	31.0	41.0
36	36.84236205006613	39.0	35.0	41.0	31.0	41.0
37	36.75587892479583	39.0	35.0	41.0	31.0	41.0
38	36.65193329663546	39.0	35.0	41.0	30.0	41.0
39	36.49572877585714	39.0	35.0	41.0	30.0	41.0
40	36.32739508899233	39.0	35.0	41.0	30.0	41.0
41	36.19596726862449	39.0	35.0	40.0	29.0	41.0
42	35.924981488397876	39.0	35.0	40.0	28.0	41.0
43	34.69204588881934	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	9.0
10	18.0
11	30.0
12	32.0
13	38.0
14	25.0
15	50.0
16	107.0
17	144.0
18	223.0
19	427.0
20	896.0
21	1686.0
22	3000.0
23	5222.0
24	8647.0
25	13490.0
26	20351.0
27	29748.0
28	41638.0
29	56362.0
30	74187.0
31	93710.0
32	118632.0
33	153869.0
34	206228.0
35	277527.0
36	406383.0
37	666544.0
38	1189971.0
39	1241425.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.981002563211646	18.087798132225977	12.218243004956815	24.712956299605562
2	17.810113255868732	21.59190235070236	35.63350020886553	24.964484184563386
3	20.340314170192222	24.561414924060138	29.367360846323987	25.73091005942365
4	14.293602902168082	15.265554192037431	33.86916125416484	36.57168165162965
5	14.32986698974672	37.79956370311858	32.832077754368065	15.038491552766633
6	34.00415388639537	34.06902582775166	16.45892896880291	15.467891317050064
7	28.35571859935601	31.33063174556491	21.872168223723392	18.441481431355683
8	27.850602369918853	33.34465501617786	20.447761711977254	18.356980901926033
9	28.140281289596068	13.608597711978993	20.003635084376903	38.24748591404804
10	17.65360508842408	26.64263086412202	32.08415263710623	23.61961141034767
11	35.29211459972212	22.025986949266283	21.362844319052833	21.31905413195877
12	21.9085841346352	26.251382134557982	29.772772523967483	22.067261206839337
13	30.13038154071186	19.351120087484944	26.249777145035964	24.268721226767234
14	22.805491319826263	20.89210523005356	26.528156070916246	29.774247379203935
15	25.72839412990265	26.95811975043714	23.535045813775234	23.778440305884978
16	23.774210941603975	25.7679766417633	26.243313808852687	24.214498607780037
17	23.364808479203024	26.568367565157153	26.15933381656531	23.90749013907451
18	23.462908041474666	24.46574453511912	28.151429460059834	23.919917963346375
19	23.902566725270475	25.21631571618753	28.262412316602838	22.618705241939157
20	23.835157165345585	24.565579221198355	28.127506440562684	23.471757172893376
21	24.628867862080213	24.959517392664157	28.014853527354877	22.396761217900753
22	24.404885067567893	24.64498282444321	27.244718825355886	23.705413282633014
23	23.099746628545997	25.11962594200956	27.988262755003557	23.79236467444089
24	23.009953971503194	26.125607347555274	27.640088473962948	23.22435020697858
25	23.564174204695156	25.370459777444342	27.281178114362874	23.78418790349762
26	23.42551612342109	25.907242016370024	27.677480392016523	22.98976146819236
27	23.713329785005147	25.788971639835147	27.08979395838566	23.40790461677405
28	22.863466144047376	26.16668640369998	27.8872568603542	23.082590591898448
29	22.94584114681273	26.14790368848281	28.0308600444799	22.87539512022456
30	22.959678759178264	26.57879999705029	27.90749274176022	22.554028502011224
31	23.514376151417316	26.023864025287697	27.184987188279585	23.276772635015405
32	22.339653955583433	26.507161072844404	27.435951157999938	23.717233813572225
33	22.305515394669094	25.99898668769637	27.953018052661875	23.742479864972665
34	23.04610961384386	26.05633252953723	27.95232400313884	22.945233853480072
35	23.06682265429697	26.092878574734602	28.04649784779581	22.793800923172622
36	22.386589054578753	26.37609415822854	27.676829720588675	23.56048706660403
37	22.56587072199803	26.008941960542415	28.037280002567982	23.38790731489157
38	22.395221295521512	25.525493089652546	28.366346232677497	23.71293938214844
39	22.38582993791293	25.265181140418797	28.50595863204574	23.843030289622526
40	22.536330239173804	25.50148331396501	28.819213546458588	23.14297290040259
41	21.482242526062645	25.823305402177844	29.115616070890216	23.578836000869295
42	22.057110732564933	26.026401643856296	28.76817921746784	23.14830840611093
43	21.329833588613422	25.263424327563612	28.786961932685006	24.61978015113796
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	982.0
1	1289.0
2	1596.0
3	5426.5
4	9257.0
5	9257.0
6	12872.0
7	16487.0
8	15894.5
9	15302.0
10	21060.5
11	26819.0
12	26819.0
13	40194.5
14	53570.0
15	67395.0
16	81220.0
17	70470.0
18	59720.0
19	59720.0
20	61280.0
21	62840.0
22	51070.0
23	39300.0
24	45155.0
25	51010.0
26	51010.0
27	57769.5
28	64529.0
29	70018.0
30	75507.0
31	83174.0
32	90841.0
33	90841.0
34	105389.5
35	119938.0
36	140500.0
37	161062.0
38	184269.0
39	207476.0
40	207476.0
41	227403.5
42	247331.0
43	265741.5
44	284152.0
45	311635.5
46	339119.0
47	339119.0
48	369203.5
49	399288.0
50	401072.5
51	402857.0
52	381503.0
53	360149.0
54	360149.0
55	346069.0
56	331989.0
57	314268.5
58	296548.0
59	274304.0
60	252060.0
61	252060.0
62	215174.0
63	178288.0
64	155218.0
65	132148.0
66	111781.0
67	91414.0
68	91414.0
69	75356.0
70	59298.0
71	50515.5
72	41733.0
73	31777.5
74	21822.0
75	21822.0
76	17398.0
77	12974.0
78	10896.5
79	8819.0
80	7143.0
81	5467.0
82	5467.0
83	4547.5
84	3628.0
85	3033.0
86	2438.0
87	1650.0
88	862.0
89	862.0
90	652.5
91	443.0
92	306.0
93	169.0
94	158.5
95	148.0
96	148.0
97	82.5
98	17.0
99	11.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4610622.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.83710765309504
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.83619564411997	42.11152595532969
2	8.86930729053362	9.017798590744764
3	2.9311627373208005	4.470355068777548
4	1.4418037260090577	2.9318852493502403
5	0.864311749136471	2.1969554718342823
6	0.5738866526748676	1.750484252562396
7	0.3738035860700342	1.3302165202308711
8	0.3019553661435573	1.22804299640558
9	0.23225834932796854	1.062660843730448
>10	1.3928459212786084	12.971649075535337
>50	0.10057436713887971	3.515109611665755
>100	0.06621103969908348	6.755586124416898
>500	0.01041321117613164	3.632696611878709
>1k	0.00501534198213214	4.367839571073817
>5k	8.500579630732118E-5	0.28400561631977195
>10k+	1.7001159261464236E-4	2.3731884401437546
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	36115	0.7832999538890849	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	28640	0.6211743231173582	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	26367	0.5718751179341963	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18711	0.40582376954779636	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6654	0.14431892269633034	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6490	0.14076191889077005	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4917	0.10664504702402408	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4781	0.10369533655112044	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	4.337809518975965E-5
3	0.0	0.0	0.0	0.0	4.337809518975965E-5
4	0.0	0.0	0.0	2.1689047594879825E-5	4.337809518975965E-5
5	0.0	0.0	0.0	6.506714278463946E-5	4.337809518975965E-5
6	2.1689047594879825E-5	0.0	0.0	6.506714278463946E-5	6.506714278463946E-5
7	2.1689047594879825E-5	0.0	0.0	8.67561903795193E-5	6.506714278463946E-5
8	2.1689047594879825E-5	0.0	0.0	8.67561903795193E-5	6.506714278463946E-5
9	2.1689047594879825E-5	0.0	0.0	1.3013428556927893E-4	6.506714278463946E-5
10	2.1689047594879825E-5	0.0	0.0	2.6026857113855786E-4	8.67561903795193E-5
11	2.1689047594879825E-5	0.0	0.0	4.1209190430271665E-4	8.67561903795193E-5
12	2.1689047594879825E-5	0.0	0.0	6.289823802515149E-4	8.67561903795193E-5
13	4.337809518975965E-5	2.1689047594879825E-5	0.0	8.024947610105535E-4	8.67561903795193E-5
14	4.337809518975965E-5	2.1689047594879825E-5	0.0	0.0013230319032876692	1.0844523797439912E-4
15	4.337809518975965E-5	2.1689047594879825E-5	0.0	0.0020604595215135833	1.952014283539184E-4
16	8.67561903795193E-5	2.1689047594879825E-5	0.0	0.004836657613658201	2.3857952354367805E-4
17	8.67561903795193E-5	2.1689047594879825E-5	0.0	0.00728751999187962	2.3857952354367805E-4
18	8.67561903795193E-5	2.1689047594879825E-5	0.0	0.008415350466813371	2.3857952354367805E-4
19	8.67561903795193E-5	4.337809518975965E-5	0.0	0.010757767607060393	2.3857952354367805E-4
20	8.67561903795193E-5	4.337809518975965E-5	0.0	0.012883294271358614	2.3857952354367805E-4
21	8.67561903795193E-5	4.337809518975965E-5	0.0	0.01826217807488881	2.3857952354367805E-4
22	8.67561903795193E-5	4.337809518975965E-5	0.0	0.02600516806626091	2.3857952354367805E-4
23	8.67561903795193E-5	4.337809518975965E-5	0.0	0.0396692680510352	3.68713809112957E-4
24	8.67561903795193E-5	4.337809518975965E-5	0.0	0.06027386326617103	3.904028567078368E-4
25	8.67561903795193E-5	4.337809518975965E-5	0.0	0.06992548944589255	3.904028567078368E-4
26	8.67561903795193E-5	4.337809518975965E-5	0.0	0.08328594276433852	3.904028567078368E-4
27	8.67561903795193E-5	4.337809518975965E-5	0.0	0.11540742225235553	3.904028567078368E-4
28	8.67561903795193E-5	4.337809518975965E-5	0.0	0.1930759016896202	3.904028567078368E-4
29	8.67561903795193E-5	4.337809518975965E-5	0.0	0.2885077111070914	3.904028567078368E-4
30	8.67561903795193E-5	4.337809518975965E-5	0.0	0.43228440761354975	3.904028567078368E-4
31	8.67561903795193E-5	4.337809518975965E-5	0.0	0.7927346895928575	3.904028567078368E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	800	0.0	14.56875	29
CTTATAC	4200	0.0	14.535714	37
GGACCGT	220	1.8189894E-11	14.295454	6
TCTAGCG	810	0.0	13.932098	28
GTCCTAT	955	0.0	13.560209	1
TCTTATA	6530	0.0	13.5137825	37
ATACCGT	785	0.0	13.433121	6
AAGACGG	925	0.0	13.4	5
GGGTACG	125	4.8505688E-5	13.32	1
GCGCAAT	870	0.0	12.971265	35
CCTATTC	1070	0.0	12.967289	3
TACCGTA	100	0.0018339353	12.950001	7
TAGCGGC	890	0.0	12.88764	30
CGCAATA	895	0.0	12.815642	36
CGGCGCA	1295	0.0	12.714286	33
TACCGTC	760	0.0	12.657895	7
GTACTAA	205	2.0701918E-8	12.634146	1
GCCGGTC	1045	0.0	12.569378	8
CATGGGT	3135	0.0	12.392344	4
TACTATA	540	0.0	12.333334	2
>>END_MODULE