FastQCFastQC Report
Thu 9 Feb 2017
SRR2727051.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727051.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4441959
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT483131.0876507414859076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT371450.8362301408004891No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT334690.7534738614201526No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260800.5871283368441716No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC108100.24336109360757271No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92450.20812889087900183No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG91200.2053148171786367No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90540.20382898626484397No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65360.14714228564468965No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65040.1464218827773962No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA62170.13996076956135795No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT62070.13973564366532876No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA58320.1312934225642335No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA57330.12906467619354434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT7550.014.9470216
AAGACGG8000.014.85
CGTTATA750.004106867614.82
CAAGACG9000.014.84
TACCGTC7150.014.2307687
GACGGAC9000.014.1833337
CGAGCCG9050.014.10497215
TCTAGCG7350.014.09523928
GTCTTAC2900.014.0344841
TACACCG1854.9094524E-914.05
GTATAGG3750.013.8133341
CTAGCGG7600.013.63157929
GTATTAG7900.013.5822781
CCTATAC3000.013.5666673
CGTCGTA6850.013.23357710
CGTATAC850.00941027713.0588243
GTCCTAT9250.013.01
CGCAATA8000.012.95000136
CGACCCG6600.012.8939395
GTACTAA2305.7661964E-1012.8695651
Produced by FastQC (version 0.11.5)