##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727050.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3566558
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1686634564754	33.0	31.0	34.0	30.0	34.0
2	32.342850726106235	34.0	31.0	34.0	30.0	34.0
3	32.45148936313387	34.0	31.0	34.0	30.0	34.0
4	36.01261804798913	37.0	35.0	37.0	35.0	37.0
5	35.799860818189416	37.0	35.0	37.0	35.0	37.0
6	35.82904918411533	37.0	35.0	37.0	35.0	37.0
7	35.79366324618862	37.0	35.0	37.0	35.0	37.0
8	35.7969507295269	37.0	35.0	37.0	35.0	37.0
9	37.57076823088255	39.0	37.0	39.0	35.0	39.0
10	37.425761756853525	39.0	37.0	39.0	35.0	39.0
11	37.4966452248919	39.0	37.0	39.0	35.0	39.0
12	37.41909089940497	39.0	37.0	39.0	35.0	39.0
13	37.45036699248968	39.0	37.0	39.0	35.0	39.0
14	38.68193058966096	40.0	38.0	41.0	35.0	41.0
15	38.5834731974077	40.0	38.0	41.0	34.0	41.0
16	38.53748207655673	40.0	38.0	41.0	34.0	41.0
17	38.565903316306645	40.0	38.0	41.0	34.0	41.0
18	38.568026652026965	40.0	38.0	41.0	34.0	41.0
19	38.624061069524174	40.0	38.0	41.0	34.0	41.0
20	38.59524421024416	40.0	38.0	41.0	34.0	41.0
21	38.49476385916057	40.0	38.0	41.0	34.0	41.0
22	38.45561294671221	40.0	38.0	41.0	34.0	41.0
23	38.36974332115165	40.0	38.0	41.0	34.0	41.0
24	38.280063579507186	40.0	38.0	41.0	34.0	41.0
25	38.24509569170051	40.0	38.0	41.0	34.0	41.0
26	38.053914165983	40.0	37.0	41.0	33.0	41.0
27	37.940821374557764	40.0	37.0	41.0	33.0	41.0
28	37.83398952154991	40.0	37.0	41.0	33.0	41.0
29	37.7157929297659	40.0	37.0	41.0	33.0	41.0
30	37.62034011503528	40.0	37.0	41.0	33.0	41.0
31	37.5277589765819	40.0	37.0	41.0	33.0	41.0
32	37.35932010638829	39.0	36.0	41.0	32.0	41.0
33	37.20176624072845	39.0	36.0	41.0	32.0	41.0
34	37.1117682650892	39.0	36.0	41.0	31.0	41.0
35	36.98679314902491	39.0	36.0	41.0	31.0	41.0
36	36.78600684469452	39.0	35.0	41.0	31.0	41.0
37	36.70267411885633	39.0	35.0	41.0	31.0	41.0
38	36.61464723130817	39.0	35.0	40.0	30.0	41.0
39	36.45399514041269	39.0	35.0	40.0	30.0	41.0
40	36.28580048326706	39.0	35.0	40.0	30.0	41.0
41	36.15538903334812	39.0	35.0	40.0	29.0	41.0
42	35.8760906173403	39.0	35.0	40.0	28.0	41.0
43	34.64650764126085	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	19.0
11	24.0
12	20.0
13	24.0
14	20.0
15	44.0
16	80.0
17	134.0
18	212.0
19	411.0
20	728.0
21	1335.0
22	2470.0
23	4156.0
24	6843.0
25	10635.0
26	16071.0
27	23393.0
28	32798.0
29	43959.0
30	57484.0
31	73034.0
32	92783.0
33	119955.0
34	161236.0
35	219222.0
36	323143.0
37	539212.0
38	964855.0
39	872249.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.96884671439522	18.219527062226383	12.180343064657857	24.63128315872054
2	17.771616219335282	21.85617057117815	35.41274248168683	24.959470727799744
3	20.618534732927376	24.60363745661784	29.18561817864731	25.59220963180747
4	14.349296997273001	15.323149097813635	33.695372401065676	36.63218150384769
5	14.360904827567644	37.87578948667034	32.735343151576394	15.02796253418562
6	34.14933950324094	33.85488193378602	16.412182277703042	15.58359628527
7	28.444763831122334	31.198511281745596	21.81436555917498	18.542359327957094
8	27.94481401956733	33.2616769445499	20.462642132835075	18.3308669030477
9	28.111557417543747	13.631602233862452	19.93008385115285	38.326756497440954
10	17.593096761639654	26.689878588824296	32.038144339724745	23.67888030981131
11	35.335356946389204	21.994146737554807	21.222394252385634	21.44810206367035
12	21.964958932393642	26.32919470256757	29.591023053599574	22.114823311439206
13	30.230266828690294	19.273456368857595	26.071915835940423	24.424360966511692
14	22.882426137469235	20.888655112295947	26.40994482635639	29.818973923878428
15	25.90534066738856	26.7565254791875	23.4524435043535	23.885690349070448
16	23.855633358549056	25.5834056252555	26.315596157415637	24.24536485877981
17	23.547493129229917	26.404561484770472	26.168115028551338	23.879830357448274
18	23.666459370631294	24.064854686226887	28.29812384938083	23.97056209376099
19	23.96565540221132	25.171551955695097	28.287245013259284	22.5755476288343
20	23.812482511149405	24.31759135839092	28.28960022520312	23.58032590525655
21	24.530738039308485	24.849841219461453	28.138277857811367	22.48114288341869
22	24.370639703602183	24.603609418380408	27.187136729586342	23.838614148431063
23	22.95047493970377	25.064389812250354	28.03933091793264	23.945804330113234
24	23.01555168877108	26.189199783096196	27.573195220714204	23.222053307418523
25	23.621177617187215	25.160028240112737	27.17079043716659	24.048003705533457
26	23.446863895105587	25.790299779226917	27.718769749433488	23.044066576234005
27	23.940000414965915	25.55169437872593	26.941213349116993	23.567091857191162
28	22.891370335208343	26.125188487051098	27.881055067659073	23.102386110081486
29	22.977896335907054	26.026241547172376	28.042134741675305	22.953727375245265
30	22.936399744515583	26.596567334668325	28.00907765974926	22.457955261066832
31	23.63225272097075	25.998848189206512	27.03242173546596	23.336477354356777
32	22.3388207902409	26.474124351826045	27.434265754265034	23.752789103668018
33	22.32642788929831	26.00204454827315	27.89482184223557	23.77670572019297
34	23.02017799794648	26.075336500906477	27.893055433277684	23.011430067869355
35	23.123274596964354	26.256014902883955	27.982833869517894	22.637876630633794
36	22.507835285448884	26.558659637667464	27.46465920363555	23.4688458732481
37	22.7498333126785	26.238182583880594	27.84025943220326	23.171724671237648
38	22.457310381606018	25.85391853994804	28.14497899655634	23.5437920818896
39	22.628007171059604	25.43068134599241	28.087248265694825	23.85406321725316
40	22.766235681573104	25.76425225665754	28.388014438570746	23.08149762319861
41	21.656454205987956	26.134693449538744	28.719314252004313	23.489538092468987
42	22.493507746123854	26.322914137384	28.327535960441413	22.856042156050734
43	21.752485169174314	25.502851769128664	28.379462776155613	24.365200285541412
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	509.0
1	783.5
2	1058.0
3	4082.0
4	7106.0
5	7106.0
6	10009.5
7	12913.0
8	12028.0
9	11143.0
10	14986.5
11	18830.0
12	18830.0
13	29362.5
14	39895.0
15	51370.0
16	62845.0
17	54350.5
18	45856.0
19	45856.0
20	47574.0
21	49292.0
22	39739.0
23	30186.0
24	34978.0
25	39770.0
26	39770.0
27	45054.0
28	50338.0
29	55111.5
30	59885.0
31	65595.5
32	71306.0
33	71306.0
34	81736.0
35	92166.0
36	107064.0
37	121962.0
38	139067.5
39	156173.0
40	156173.0
41	171602.0
42	187031.0
43	200592.0
44	214153.0
45	237100.0
46	260047.0
47	260047.0
48	290006.0
49	319965.0
50	320499.0
51	321033.0
52	298795.0
53	276557.0
54	276557.0
55	265310.5
56	254064.0
57	241300.0
58	228536.0
59	212697.5
60	196859.0
61	196859.0
62	167689.5
63	138520.0
64	120403.5
65	102287.0
66	87141.0
67	71995.0
68	71995.0
69	59395.5
70	46796.0
71	39775.0
72	32754.0
73	24994.0
74	17234.0
75	17234.0
76	13591.0
77	9948.0
78	8496.5
79	7045.0
80	5643.5
81	4242.0
82	4242.0
83	3620.0
84	2998.0
85	2512.0
86	2026.0
87	1325.0
88	624.0
89	624.0
90	488.5
91	353.0
92	250.0
93	147.0
94	118.5
95	90.0
96	90.0
97	52.5
98	15.0
99	10.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3566558.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.33126373546079
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.70149913462429	43.802052262674664
2	8.623931012355305	9.026024164879695
3	2.704593195469501	4.246043394277413
4	1.3656028139919265	2.8585488406759563
5	0.7822212859330516	2.0467314206826908
6	0.5377570320358954	1.6884903041441444
7	0.37523564866820613	1.3745588985381818
8	0.2874611818231911	1.203456553575733
9	0.2130791806762919	1.0035632520456237
>10	1.240410545646377	11.713513525314204
>50	0.08669601030021772	3.115092525385591
>100	0.065930392323955	7.224937589163435
>500	0.010512895660331721	3.7720396591130543
>1k	0.004802845728780804	4.3284483195202945
>5k	5.336495254200893E-5	0.15348434109761375
>10k+	2.1345981016803573E-4	2.4430149489117996
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	29095	0.8157725179290509	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	22546	0.6321501010217694	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	20741	0.5815410824666247	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15098	0.42332130866790896	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5496	0.1540981528969948	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4998	0.14013511065851164	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	4893	0.137191095728711	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	4050	0.11355486157802565	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3870	0.10850797884122451	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3818	0.10704999049503752	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3572	0.10015258408807597	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.8038237426673E-5	0.0	0.0	0.0	2.8038237426673E-5
3	2.8038237426673E-5	0.0	0.0	0.0	2.8038237426673E-5
4	2.8038237426673E-5	0.0	0.0	2.8038237426673E-5	8.4114712280019E-5
5	2.8038237426673E-5	0.0	0.0	2.8038237426673E-5	8.4114712280019E-5
6	2.8038237426673E-5	0.0	0.0	2.8038237426673E-5	8.4114712280019E-5
7	2.8038237426673E-5	0.0	0.0	2.8038237426673E-5	8.4114712280019E-5
8	2.8038237426673E-5	0.0	0.0	5.6076474853346E-5	8.4114712280019E-5
9	2.8038237426673E-5	0.0	0.0	5.6076474853346E-5	1.68229424560038E-4
10	2.8038237426673E-5	0.0	0.0	1.96267661986711E-4	1.68229424560038E-4
11	2.8038237426673E-5	0.0	0.0	2.8038237426673E-4	1.68229424560038E-4
12	2.8038237426673E-5	0.0	0.0	3.64497086546749E-4	1.68229424560038E-4
13	2.8038237426673E-5	0.0	0.0	5.32726511106787E-4	1.68229424560038E-4
14	2.8038237426673E-5	0.0	0.0	8.97223597653536E-4	2.52344136840057E-4
15	2.8038237426673E-5	0.0	0.0	0.001317797159053631	3.08420611693403E-4
16	2.8038237426673E-5	0.0	0.0	0.002887938454947319	3.36458849120076E-4
17	2.8038237426673E-5	0.0	0.0	0.004402003275987661	3.36458849120076E-4
18	2.8038237426673E-5	0.0	0.0	0.005102959211654486	3.64497086546749E-4
19	2.8038237426673E-5	0.0	0.0	0.006588985795268155	3.64497086546749E-4
20	2.8038237426673E-5	0.0	0.0	0.008299318278295208	3.92535323973422E-4
21	2.8038237426673E-5	0.0	0.0	0.012028403856042718	3.92535323973422E-4
22	2.8038237426673E-5	0.0	0.0	0.018365045514470813	3.92535323973422E-4
23	2.8038237426673E-5	0.0	0.0	0.030533640557646898	5.32726511106787E-4
24	2.8038237426673E-5	2.8038237426673E-5	0.0	0.048085577186744194	5.32726511106787E-4
25	2.8038237426673E-5	2.8038237426673E-5	0.0	0.05604843661591933	5.6076474853346E-4
26	2.8038237426673E-5	2.8038237426673E-5	0.0	0.06771234338541529	5.6076474853346E-4
27	2.8038237426673E-5	2.8038237426673E-5	0.0	0.09213364818404747	5.6076474853346E-4
28	2.8038237426673E-5	2.8038237426673E-5	0.0	0.14801385537540676	5.6076474853346E-4
29	2.8038237426673E-5	2.8038237426673E-5	0.0	0.21550189286140867	5.6076474853346E-4
30	2.8038237426673E-5	2.8038237426673E-5	0.0	0.3159909357986047	5.6076474853346E-4
31	2.8038237426673E-5	2.8038237426673E-5	0.0	0.577139079190637	5.6076474853346E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	615	0.0	17.747969	29
GTAATAC	240	0.0	16.958334	3
TCTAGCG	650	0.0	16.792309	28
CGCAATA	670	0.0	16.291044	36
TAATACT	450	0.0	16.033333	4
AAGACGG	650	0.0	15.938462	5
GCGCAAT	715	0.0	15.783216	35
TAGCGGC	695	0.0	15.438849	30
CAAGACG	705	0.0	14.957447	4
GGCGCAA	950	0.0	14.8	34
CGGCGCA	960	0.0	14.645834	33
GACGTCG	215	1.2732926E-11	14.627907	37
CGAACGA	455	0.0	14.23077	16
TCTATAC	425	0.0	13.929411	3
CGGTCCA	800	0.0	13.64375	10
ATACCGT	665	0.0	13.631578	6
ACGAACG	475	0.0	13.631578	15
TAATACC	315	0.0	13.5079365	4
GTTACGG	440	0.0	13.454545	25
GCAATAC	800	0.0	13.4125	37
>>END_MODULE