FastQCFastQC Report
Thu 9 Feb 2017
SRR2727049.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727049.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2257893
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT330201.4624253673668326No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT228151.0104553227278708No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT203690.9021242370652639No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155420.6883408558332924No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC53390.23645938935104543No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44010.1949162338516484No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT42200.18689991066892894No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG42160.18672275435549868No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42130.18658988712042598No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA39250.17383463255344697No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37840.16758987250503013No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA35470.15709336093428697No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA31210.1382262135539638No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA30530.1352145562256493No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG24590.10890684368125504No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG24170.10704670239023728No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCGC609.2405785E-418.519
TAGTACT1502.5102054E-1017.2666664
AAGACGG5450.015.9541285
GTATACG750.004106036414.81
TACTAGT1001.0941057E-414.82
CAAGACG5750.014.4782614
ACTATAC1803.3360266E-914.3888893
CTCTATA1551.2116652E-714.322582
ACGGACC6350.013.6929128
CGAACGT2051.4260877E-913.5365854
GTTCTAG1102.4580056E-413.4545451
TCTAGCG3200.013.29687628
CTACTAG1409.567784E-613.2142851
CTTATAC18250.013.178081537
AGACGGA6350.013.1102356
TAGACTC1703.7355858E-713.0588245
TCTATAC2850.012.9824563
CCGATAA2305.7661964E-1012.8695659
TACCGTC4250.012.6235297
GACGGAC6750.012.6074087
Produced by FastQC (version 0.11.5)