##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727049.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2257893
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.01440147960953	31.0	31.0	33.0	28.0	34.0
2	31.142583373082783	31.0	31.0	34.0	28.0	34.0
3	31.210594124699444	31.0	31.0	34.0	28.0	34.0
4	35.03557918820777	35.0	35.0	37.0	32.0	37.0
5	34.22937003657835	35.0	35.0	37.0	30.0	37.0
6	34.302067901357596	35.0	35.0	37.0	30.0	37.0
7	34.23181125057742	35.0	35.0	37.0	30.0	37.0
8	34.22112828198679	35.0	35.0	37.0	30.0	37.0
9	35.75146342187163	37.0	35.0	39.0	30.0	39.0
10	35.39503820597344	37.0	35.0	39.0	30.0	39.0
11	35.62750316334742	37.0	35.0	39.0	30.0	39.0
12	35.47623115887245	37.0	35.0	39.0	30.0	39.0
13	35.581053663747575	37.0	35.0	39.0	30.0	39.0
14	36.355064212520254	38.0	35.0	40.0	31.0	41.0
15	36.214822845901026	38.0	35.0	40.0	30.0	41.0
16	36.08453323518874	38.0	34.0	40.0	30.0	41.0
17	36.19771486071306	38.0	35.0	40.0	30.0	41.0
18	36.196783461395206	38.0	35.0	40.0	30.0	41.0
19	36.27163510405497	38.0	35.0	40.0	30.0	41.0
20	36.24520205341883	38.0	35.0	40.0	30.0	41.0
21	36.102371990169594	38.0	34.0	40.0	29.0	41.0
22	36.07417534843325	38.0	34.0	40.0	30.0	41.0
23	35.92607355618712	38.0	34.0	40.0	29.0	41.0
24	35.781530391387015	38.0	34.0	40.0	29.0	41.0
25	35.744839104421686	38.0	34.0	40.0	29.0	41.0
26	35.40740017352461	38.0	34.0	40.0	27.0	41.0
27	35.233872021393395	38.0	34.0	40.0	27.0	41.0
28	35.06931196473881	37.0	33.0	40.0	27.0	40.0
29	34.86675187885343	37.0	33.0	39.0	26.0	40.0
30	34.68565560901247	37.0	33.0	39.0	26.0	40.0
31	34.53927001855269	37.0	33.0	39.0	25.0	40.0
32	34.26262670551704	37.0	33.0	39.0	25.0	40.0
33	34.02885344876839	36.0	33.0	39.0	24.0	40.0
34	33.874584845251746	36.0	33.0	39.0	24.0	40.0
35	33.66391232888361	36.0	32.0	39.0	23.0	40.0
36	33.357645822897716	36.0	31.0	39.0	21.0	40.0
37	33.21477412791483	36.0	31.0	39.0	21.0	40.0
38	33.04825383665214	36.0	31.0	39.0	21.0	40.0
39	32.7930424515245	35.0	31.0	39.0	19.0	40.0
40	32.52195476047802	35.0	31.0	39.0	18.0	40.0
41	32.28622614091987	35.0	30.0	39.0	16.0	40.0
42	31.879554079843466	35.0	30.0	38.0	15.0	40.0
43	30.68555020100598	34.0	27.0	38.0	11.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	9.0
10	14.0
11	17.0
12	9.0
13	30.0
14	63.0
15	123.0
16	207.0
17	382.0
18	731.0
19	1368.0
20	2536.0
21	4311.0
22	7219.0
23	11173.0
24	16559.0
25	24232.0
26	33326.0
27	45037.0
28	57803.0
29	73682.0
30	91600.0
31	113466.0
32	140717.0
33	177001.0
34	223146.0
35	282153.0
36	338638.0
37	331558.0
38	223141.0
39	57641.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.30303251748422	19.298788737996	12.864161410660294	22.534017333859488
2	17.627673233408313	24.366655107217216	33.98540143399178	24.020270225382692
3	22.076289709034043	26.591605536666265	29.108420992491673	22.223683761808022
4	15.379913928605122	16.07848556153901	32.327971254616585	36.21362925523929
5	13.754681909195874	39.98670441867706	31.479968271304266	14.778645400822802
6	33.18890664880931	33.41230076004487	16.69538813398155	16.703404457164268
7	26.951232852929703	32.50667768578936	22.215933173095447	18.326156288185487
8	28.93719941556132	32.551276787695436	20.967866944979235	17.543656851764013
9	28.772886934854753	13.18725023727874	20.77286213297087	37.26700069489564
10	18.21348487284384	28.158863152505454	31.20905197899103	22.418599995659672
11	33.507655145748714	23.030542191326163	21.17646850404337	22.285334158881753
12	21.698946761427578	28.294742044906467	29.616505299409674	20.389805894256284
13	29.977992756964127	19.47594505142626	27.4057716641134	23.14029052749621
14	22.765117744729267	21.7520493663783	28.105849125711448	27.376983763180984
15	26.05703636089044	26.697146410392342	25.052604352819195	22.193212875898016
16	21.84926389337316	26.001453567551696	28.792551285645512	23.356731253429636
17	22.393399510074214	27.361482585755837	27.838431670588466	22.406686233581485
18	22.188828257140617	24.028729439348986	30.8708162875743	22.911626015936097
19	22.546462564877963	25.727171305283285	31.049611296903795	20.676754832934954
20	22.500401923386097	24.752235823398188	31.0593106050641	21.688051648151617
21	23.01712260058382	25.431940308951752	30.946683478800814	20.604253611663616
22	22.948828841756452	25.453730535503677	29.220029469952742	22.377411152787133
23	21.285021035097763	26.134542248016178	30.573813728108462	22.00662298877759
24	21.197461527184856	27.69241057924357	29.80398096809725	21.306146925474327
25	21.88597953933158	26.449481884216837	29.237745101295765	22.426793475155822
26	21.85382566844399	26.833025302793356	30.115953236047943	21.197195792714712
27	21.996525078912065	26.87292976239352	29.143453653472505	21.987091505221905
28	21.079652578753734	27.617340591427496	30.450512933961	20.852493895857776
29	21.327095659537456	27.214487134687072	30.50197684301249	20.956440362762983
30	21.156582707860824	28.382434420054448	30.25652677075486	20.204456101329868
31	22.00352275329256	27.738249775343647	29.123257833741455	21.13496963762233
32	20.143691485823286	28.433189703852218	29.79990637288835	21.62321243743614
33	20.360132211756714	28.03427797508562	30.181722517408932	21.423867295748735
34	20.6784821069909	28.07524537256637	29.99464545042657	21.25162707001616
35	20.830127911287207	28.567474189432364	29.97865709313949	20.623740806140948
36	20.663468109427683	28.630187524386674	29.111964118760277	21.594380247425367
37	20.56089460395156	28.74777502742601	29.55299476104492	21.138335607577506
38	20.805060292936822	28.110410900782277	29.583111334328066	21.501417471952834
39	20.79726541514589	27.574911654360946	29.47278724013937	22.155035690353795
40	20.92610234408805	28.161564786285265	29.5806754350184	21.331657434608285
41	19.839824119212025	28.537401905227572	29.714649897050037	21.90812407851036
42	20.759796854855388	28.868374187793666	29.17011567864376	21.201713278707185
43	20.188512033121146	27.835508591416865	29.185616856068908	22.79036251939308
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	350.0
1	562.5
2	775.0
3	2708.5
4	4642.0
5	4642.0
6	7637.5
7	10633.0
8	11478.0
9	12323.0
10	16304.0
11	20285.0
12	20285.0
13	32212.5
14	44140.0
15	60204.5
16	76269.0
17	64766.5
18	53264.0
19	53264.0
20	53494.0
21	53724.0
22	42158.0
23	30592.0
24	33117.5
25	35643.0
26	35643.0
27	38448.5
28	41254.0
29	42791.0
30	44328.0
31	46360.0
32	48392.0
33	48392.0
34	52983.0
35	57574.0
36	66024.5
37	74475.0
38	83776.5
39	93078.0
40	93078.0
41	101269.0
42	109460.0
43	118672.0
44	127884.0
45	143644.0
46	159404.0
47	159404.0
48	183636.0
49	207868.0
50	204168.0
51	200468.0
52	177491.5
53	154515.0
54	154515.0
55	146308.5
56	138102.0
57	130961.5
58	123821.0
59	114292.5
60	104764.0
61	104764.0
62	89109.5
63	73455.0
64	63474.0
65	53493.0
66	45391.0
67	37289.0
68	37289.0
69	30979.5
70	24670.0
71	20891.5
72	17113.0
73	13142.0
74	9171.0
75	9171.0
76	7316.0
77	5461.0
78	4510.5
79	3560.0
80	2930.0
81	2300.0
82	2300.0
83	1946.5
84	1593.0
85	1316.0
86	1039.0
87	735.5
88	432.0
89	432.0
90	300.5
91	169.0
92	117.5
93	66.0
94	56.5
95	47.0
96	47.0
97	26.5
98	6.0
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2257893.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.175301368899305
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.45413070381218	45.44049153392525
2	7.656757354549802	8.143007596734439
3	2.4781598087479924	3.9533068401140516
4	1.2294354840015138	2.615024095015963
5	0.7244262494532477	1.9260792067108938
6	0.48538614433270044	1.5486332703107646
7	0.3179929189636254	1.1836558509346733
8	0.2457163145466562	1.0452831261818962
9	0.18618268020078121	0.891028811641135
>10	1.0456163206405518	10.175694700656113
>50	0.0880055741768785	3.2853327529524217
>100	0.07500199743278009	8.316639978967551
>500	0.00895819186947232	3.2373143593524563
>1k	0.0038155261666270924	3.9563891989306774
>5k	8.294622101363245E-5	0.23548675510692177
>10k+	3.317848840545298E-4	4.046631922464814
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	33020	1.4624253673668326	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	22815	1.0104553227278708	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	20369	0.9021242370652639	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15542	0.6883408558332924	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	5339	0.23645938935104543	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4401	0.1949162338516484	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4220	0.18689991066892894	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	4216	0.18672275435549868	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4213	0.18658988712042598	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	3925	0.17383463255344697	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3784	0.16758987250503013	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	3547	0.15709336093428697	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	3121	0.1382262135539638	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA	3053	0.1352145562256493	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	2459	0.10890684368125504	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	2417	0.10704670239023728	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.4289078357566104E-5
2	0.0	0.0	0.0	0.0	4.4289078357566104E-5
3	0.0	0.0	0.0	0.0	4.4289078357566104E-5
4	0.0	0.0	0.0	0.0	4.4289078357566104E-5
5	0.0	0.0	0.0	4.4289078357566104E-5	4.4289078357566104E-5
6	0.0	0.0	0.0	4.4289078357566104E-5	4.4289078357566104E-5
7	8.857815671513221E-5	0.0	0.0	4.4289078357566104E-5	4.4289078357566104E-5
8	8.857815671513221E-5	0.0	0.0	8.857815671513221E-5	4.4289078357566104E-5
9	8.857815671513221E-5	0.0	0.0	8.857815671513221E-5	4.4289078357566104E-5
10	8.857815671513221E-5	0.0	0.0	8.857815671513221E-5	4.4289078357566104E-5
11	8.857815671513221E-5	0.0	0.0	2.2144539178783052E-4	4.4289078357566104E-5
12	8.857815671513221E-5	0.0	0.0	2.2144539178783052E-4	4.4289078357566104E-5
13	8.857815671513221E-5	0.0	0.0	3.100235485029627E-4	4.4289078357566104E-5
14	1.328672350726983E-4	0.0	0.0	9.300706455088882E-4	4.4289078357566104E-5
15	1.328672350726983E-4	0.0	0.0	0.001727274055945078	4.4289078357566104E-5
16	1.328672350726983E-4	0.0	0.0	0.004606064149186875	4.4289078357566104E-5
17	1.328672350726983E-4	0.0	0.0	0.006953385302137878	4.4289078357566104E-5
18	1.328672350726983E-4	0.0	0.0	0.008326346731222426	4.4289078357566104E-5
19	1.328672350726983E-4	0.0	0.0	0.011780894843112583	4.4289078357566104E-5
20	1.328672350726983E-4	0.0	0.0	0.015102575719930041	4.4289078357566104E-5
21	1.328672350726983E-4	0.0	0.0	0.02294174258921924	4.4289078357566104E-5
22	1.328672350726983E-4	0.0	0.0	0.034191168492041034	4.4289078357566104E-5
23	1.328672350726983E-4	0.0	0.0	0.055538504260387894	8.857815671513221E-5
24	1.328672350726983E-4	0.0	0.0	0.08676230450247199	8.857815671513221E-5
25	1.328672350726983E-4	0.0	0.0	0.1017763020656869	8.857815671513221E-5
26	1.328672350726983E-4	0.0	0.0	0.1263567405541361	1.328672350726983E-4
27	1.328672350726983E-4	0.0	0.0	0.15793485342308072	1.328672350726983E-4
28	1.328672350726983E-4	0.0	0.0	0.22100250100425486	1.328672350726983E-4
29	1.328672350726983E-4	0.0	0.0	0.2915107137495001	1.328672350726983E-4
30	1.328672350726983E-4	0.0	0.0	0.4000632448038946	1.328672350726983E-4
31	1.328672350726983E-4	0.0	0.0	0.6696065756880419	1.328672350726983E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTCGC	60	9.2405785E-4	18.5	19
TAGTACT	150	2.5102054E-10	17.266666	4
AAGACGG	545	0.0	15.954128	5
GTATACG	75	0.0041060364	14.8	1
TACTAGT	100	1.0941057E-4	14.8	2
CAAGACG	575	0.0	14.478261	4
ACTATAC	180	3.3360266E-9	14.388889	3
CTCTATA	155	1.2116652E-7	14.32258	2
ACGGACC	635	0.0	13.692912	8
CGAACGT	205	1.4260877E-9	13.536585	4
GTTCTAG	110	2.4580056E-4	13.454545	1
TCTAGCG	320	0.0	13.296876	28
CTACTAG	140	9.567784E-6	13.214285	1
CTTATAC	1825	0.0	13.1780815	37
AGACGGA	635	0.0	13.110235	6
TAGACTC	170	3.7355858E-7	13.058824	5
TCTATAC	285	0.0	12.982456	3
CCGATAA	230	5.7661964E-10	12.869565	9
TACCGTC	425	0.0	12.623529	7
GACGGAC	675	0.0	12.607408	7
>>END_MODULE