FastQCFastQC Report
Thu 9 Feb 2017
SRR2727048.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727048.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2061706
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT302231.4659219112715391No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT231011.1204798356312684No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT214091.0384118783182472No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166820.8091357351630154No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64760.31410880115787604No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59930.2906816005773859No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA43500.21099031578702296No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43440.2106992946617995No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC42450.20589744609561209No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42310.20521839680342396No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA40010.19406258700319057No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT39660.19236496377272025No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG34760.16859823854613606No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA29120.14124225277512895No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA23790.11538987615110981No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG20970.10171188326560626No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA20850.10112984101515929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC509.095664E-625.8999983
CCCTATA806.965638E-720.81252
CTAGACG450.00382682320.5555553
TCGTATA609.240204E-418.52
CACGCGT500.00703673218.49999810
AATCGGT500.00703673218.49999819
AAGACGG3500.017.4428565
TATAGGG1005.882346E-616.652
TATACTG2050.016.2439025
TATACCG700.0025934315.8571425
AACCGTG957.0640206E-515.5789487
CAAGACG4000.015.2624994
TATATCT1701.4879333E-915.2352934
GTCTTAC855.366717E-415.2352931
GTATTAG2950.015.0508471
ACCGTCG2950.015.0508478
ATACCGT3250.014.8000016
CGTTAAT750.004105875314.81
TACCGTC3150.014.682547
CGAGCCG3650.014.19178115
Produced by FastQC (version 0.11.5)