FastQCFastQC Report
Thu 9 Feb 2017
SRR2727047.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727047.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2936448
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT318371.0842010483413975No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT236190.804339119916307No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT215720.7346290484285777No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172140.5862184516803975No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC145230.4945771217470904No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG120820.4114494790985572No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA78150.2661378645220348No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59670.20320468811298548No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58610.19959488470424133No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG49750.16942237696700232No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG47380.16135140142103657No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG43150.146946242535199No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42470.14463051959374046No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT41880.1426212893945338No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT41520.14139531842552636No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40000.13621899655638375No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA38530.13121294843293665No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG38140.1298848132165119No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC35110.11956622422736585No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC34490.1174548297807419No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA32390.11030333246153175No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA30790.10485457259927641No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG852.7254513E-517.4117645
GTATAGT2350.017.3191491
CGCTTCG5000.016.27999932
CACTCTA5700.015.5789479
CTTATAC27850.014.9461437
CTTAGCG1001.09428336E-414.83
AAGACGG4800.014.6458345
CGAACGA3300.014.57575716
GTAAACG5600.014.53571527
CCGATAA3350.014.358219
GTATAGG2900.014.0344831
TAACGAA3700.014.013
ACGAACG3450.013.94202815
TACCGTT1203.3037944E-513.8750017
CGATAAC3600.013.87510
TACCGCA6600.013.73484828
TAAACGC6150.013.53658528
CGAGCCG6700.013.52985215
CCTTATA2355.638867E-1113.3829792
AGTACTC7550.013.2317895
Produced by FastQC (version 0.11.5)