##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727047.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2936448
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.096462460768926	33.0	31.0	34.0	30.0	34.0
2	32.24956682359095	33.0	31.0	34.0	30.0	34.0
3	32.35418369404124	34.0	31.0	34.0	30.0	34.0
4	35.930530695588686	37.0	35.0	37.0	35.0	37.0
5	35.709032136785666	37.0	35.0	37.0	33.0	37.0
6	35.75843774519419	37.0	35.0	37.0	35.0	37.0
7	35.710463117344496	37.0	35.0	37.0	33.0	37.0
8	35.73227586526307	37.0	35.0	37.0	33.0	37.0
9	37.486207145503684	39.0	37.0	39.0	35.0	39.0
10	37.33275917026285	39.0	37.0	39.0	34.0	39.0
11	37.414944518002706	39.0	37.0	39.0	35.0	39.0
12	37.34601906793514	39.0	37.0	39.0	34.0	39.0
13	37.3791890746916	39.0	37.0	39.0	34.0	39.0
14	38.568314167320516	40.0	38.0	41.0	34.0	41.0
15	38.466136298003576	40.0	38.0	41.0	34.0	41.0
16	38.387076835687196	40.0	38.0	41.0	34.0	41.0
17	38.46221387199773	40.0	38.0	41.0	34.0	41.0
18	38.46678640316464	40.0	38.0	41.0	34.0	41.0
19	38.514179716446534	40.0	38.0	41.0	34.0	41.0
20	38.504960755307096	40.0	38.0	41.0	34.0	41.0
21	38.39491964441393	40.0	38.0	41.0	34.0	41.0
22	38.37145285732967	40.0	38.0	41.0	34.0	41.0
23	38.271247098535376	40.0	38.0	41.0	34.0	41.0
24	38.17529035079116	40.0	38.0	41.0	34.0	41.0
25	38.148720494965346	40.0	37.0	41.0	34.0	41.0
26	37.91677189584151	40.0	37.0	41.0	33.0	41.0
27	37.79759287411185	40.0	37.0	41.0	33.0	41.0
28	37.67578891231856	40.0	37.0	41.0	33.0	41.0
29	37.53962848993069	39.0	37.0	41.0	33.0	41.0
30	37.409828132492045	39.0	36.0	41.0	32.0	41.0
31	37.28225393400462	39.0	36.0	41.0	32.0	41.0
32	37.04982141689551	39.0	35.0	41.0	31.0	41.0
33	36.889258042369555	39.0	35.0	40.0	31.0	41.0
34	36.76630984100519	39.0	35.0	40.0	31.0	41.0
35	36.62719380693954	39.0	35.0	40.0	31.0	41.0
36	36.41639967743342	39.0	35.0	40.0	30.0	41.0
37	36.301441741859556	39.0	35.0	40.0	30.0	41.0
38	36.18628799147814	39.0	35.0	40.0	30.0	41.0
39	36.00291474597881	39.0	35.0	40.0	29.0	41.0
40	35.78250696079073	39.0	35.0	40.0	27.0	41.0
41	35.62165956965695	39.0	35.0	40.0	27.0	41.0
42	35.299791789263764	38.0	35.0	40.0	25.0	41.0
43	34.05522386229894	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	18.0
11	22.0
12	25.0
13	16.0
14	22.0
15	45.0
16	71.0
17	105.0
18	214.0
19	331.0
20	699.0
21	1360.0
22	2312.0
23	3996.0
24	6491.0
25	10223.0
26	15735.0
27	22842.0
28	31395.0
29	41896.0
30	53727.0
31	67234.0
32	83991.0
33	107980.0
34	142500.0
35	188694.0
36	278319.0
37	459172.0
38	777743.0
39	639260.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.8089160782006	18.11307402685149	12.252285754762216	23.825724140185695
2	17.004013011638552	23.04011513229589	34.89661659256353	25.059255263502028
3	21.255544113159843	25.859644032518197	29.078192428403295	23.806619425918658
4	14.636084139749794	15.881364151519115	32.768535318861424	36.71401638986966
5	13.82633031472037	38.89569983871671	32.475562312017786	14.802407534545136
6	33.52400587376313	32.97977692777124	17.056865982302426	16.4393512161632
7	27.31405425875071	31.71300836929515	22.37151824244802	18.601419129506127
8	28.56134350071924	32.598091299420254	21.14609214942679	17.69447305043372
9	28.481042402249244	13.520586776949568	20.246876498408962	37.751494322392226
10	17.9084730940238	27.59861574255699	31.622116243842903	22.870794919576305
11	33.83856959156096	22.819406303125408	20.912783063074844	22.429241042238786
12	21.890222472865176	27.545626552896564	29.60948056972233	20.954670404515934
13	30.25022067477442	19.307476243407002	26.4382001656423	24.00410291617628
14	22.918846170611566	21.6399881761911	27.03742753149383	28.403738121703498
15	26.645866025892506	26.088526001482066	24.323536463100996	22.942071509524432
16	22.497350540516976	25.804407229414583	28.292923968004878	23.405318262063552
17	23.454288991325576	26.950894413931387	27.084286866309228	22.51052972843381
18	23.38778006625692	23.620408057626086	29.739910258925068	23.251901617191926
19	23.078086177585984	26.12935764569984	29.680689050172184	21.111867126542
20	22.962981125495837	24.767303899132557	29.901363824593524	22.368351150778082
21	23.24199168519245	25.529687568109498	29.687125397759466	21.541195348938583
22	23.36360119436816	25.398474619676563	27.59888818055011	23.63903600540517
23	21.687188058497885	25.873334041672113	29.021014504598753	23.418463395231246
24	21.734285776557254	27.91484814306264	28.335424294930473	22.01544178544963
25	22.75190979033172	25.72638779913692	27.590476657512752	23.931225753018612
26	22.756507181465498	26.321971306830566	28.75358255960943	22.167938952094506
27	23.121948694477137	26.17230068436424	27.512423172485946	23.193327448672683
28	21.857053147203697	26.90062960420208	29.151955015038578	22.090362233555645
29	22.285121343882132	26.360725611350855	29.38720522209145	21.966947822675557
30	21.789182032169478	27.87061102393095	29.36237930996905	20.977827633930517
31	23.349911185214246	26.566041693910464	27.55162018874504	22.53242693213025
32	20.59059108146986	27.596776786103483	28.80953451244497	23.00309761998169
33	21.262048570245412	27.310512564840245	28.894943823285818	22.532495041628525
34	21.431130399721024	27.000444073928776	29.058951495139706	22.509474031210498
35	21.44699991281984	27.856342084041675	29.399396822283247	21.297261180855237
36	21.817345309707513	27.344465149731924	28.303344721241448	22.534844819319122
37	21.471349058454294	27.451294897781263	28.945242687764267	22.132113356000175
38	21.67322561135086	26.91925755198117	29.095832788457347	22.31168404821063
39	21.720800095898174	25.900680005230807	28.863272906586463	23.515246992284556
40	21.4810206072098	26.90529510483414	29.353116418203218	22.260567869752844
41	20.43744687459134	27.029118172703892	29.377125016346277	23.156309936358486
42	21.460008826990975	27.387680626389432	29.193093151998607	21.959217394620982
43	20.87263251383985	25.971173335948738	29.26188374525958	23.89431040495183
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	364.0
1	685.5
2	1007.0
3	4217.0
4	7427.0
5	7427.0
6	10726.0
7	14025.0
8	13452.0
9	12879.0
10	17587.5
11	22296.0
12	22296.0
13	34331.5
14	46367.0
15	58982.0
16	71597.0
17	60855.0
18	50113.0
19	50113.0
20	51206.5
21	52300.0
22	40805.5
23	29311.0
24	33094.5
25	36878.0
26	36878.0
27	40981.5
28	45085.0
29	47882.5
30	50680.0
31	54438.5
32	58197.0
33	58197.0
34	65714.5
35	73232.0
36	83841.0
37	94450.0
38	107782.0
39	121114.0
40	121114.0
41	132302.5
42	143491.0
43	155561.0
44	167631.0
45	192370.5
46	217110.0
47	217110.0
48	264218.5
49	311327.0
50	308160.0
51	304993.0
52	256171.5
53	207350.0
54	207350.0
55	195354.5
56	183359.0
57	174302.0
58	165245.0
59	152792.0
60	140339.0
61	140339.0
62	119466.5
63	98594.0
64	85906.5
65	73219.0
66	62075.0
67	50931.0
68	50931.0
69	41726.5
70	32522.0
71	27400.5
72	22279.0
73	17068.0
74	11857.0
75	11857.0
76	9387.5
77	6918.0
78	5860.0
79	4802.0
80	3861.0
81	2920.0
82	2920.0
83	2468.5
84	2017.0
85	1672.5
86	1328.0
87	905.0
88	482.0
89	482.0
90	354.0
91	226.0
92	165.0
93	104.0
94	89.0
95	74.0
96	74.0
97	39.5
98	5.0
99	4.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2936448.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.1043432569523
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.9402488600748	41.218307930991124
2	8.269078572462279	8.120953452817936
3	2.7527140743215504	4.0551065038118725
4	1.3183644990231258	2.589496915912461
5	0.8003456917482296	1.9650224785914001
6	0.5367958256895783	1.5815403890136113
7	0.3957060494887604	1.360161997806405
8	0.27716828000501886	1.088813308904441
9	0.22085655416167962	0.9760514441492514
>10	1.2874333470776749	11.666767585231685
>50	0.10138019047284855	3.4675481207684746
>100	0.08362044866797984	8.466739078943846
>500	0.009938200763306504	3.3591951679427656
>1k	0.005728268495508613	5.323159559016755
>5k	2.0704584923525107E-4	0.6656914780152421
>10k+	4.1409169847050214E-4	4.095444588082674
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	31837	1.0842010483413975	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23619	0.804339119916307	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	21572	0.7346290484285777	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17214	0.5862184516803975	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	14523	0.4945771217470904	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	12082	0.4114494790985572	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	7815	0.2661378645220348	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5967	0.20320468811298548	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5861	0.19959488470424133	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	4975	0.16942237696700232	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	4738	0.16135140142103657	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	4315	0.146946242535199	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4247	0.14463051959374046	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	4188	0.1426212893945338	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4152	0.14139531842552636	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4000	0.13621899655638375	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	3853	0.13121294843293665	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	3814	0.1298848132165119	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	3511	0.11956622422736585	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	3449	0.1174548297807419	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	3239	0.11030333246153175	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	3079	0.10485457259927641	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.405474913909594E-5	0.0
3	0.0	0.0	0.0	3.405474913909594E-5	0.0
4	0.0	0.0	0.0	3.405474913909594E-5	0.0
5	0.0	3.405474913909594E-5	0.0	3.405474913909594E-5	0.0
6	0.0	3.405474913909594E-5	0.0	3.405474913909594E-5	6.810949827819188E-5
7	0.0	3.405474913909594E-5	0.0	3.405474913909594E-5	6.810949827819188E-5
8	0.0	3.405474913909594E-5	0.0	6.810949827819188E-5	1.0216424741728782E-4
9	0.0	3.405474913909594E-5	0.0	6.810949827819188E-5	1.702737456954797E-4
10	0.0	3.405474913909594E-5	0.0	1.702737456954797E-4	1.702737456954797E-4
11	0.0	3.405474913909594E-5	0.0	5.44875986225535E-4	1.702737456954797E-4
12	0.0	3.405474913909594E-5	0.0	9.194782267555905E-4	1.702737456954797E-4
13	0.0	3.405474913909594E-5	0.0	0.00108975197245107	1.702737456954797E-4
14	0.0	3.405474913909594E-5	0.0	0.0018730112026502767	2.0432849483457564E-4
15	0.0	6.810949827819188E-5	0.0	0.002894653676823155	2.0432849483457564E-4
16	0.0	6.810949827819188E-5	0.0	0.006232019092454557	2.0432849483457564E-4
17	0.0	6.810949827819188E-5	0.0	0.008956399023582232	2.0432849483457564E-4
18	0.0	6.810949827819188E-5	0.0	0.010591026982258838	2.3838324397367159E-4
19	0.0	6.810949827819188E-5	0.0	0.01460948738067216	2.3838324397367159E-4
20	0.0	6.810949827819188E-5	0.0	0.018662002528224576	2.3838324397367159E-4
21	0.0	6.810949827819188E-5	0.0	0.027141635063859467	2.3838324397367159E-4
22	0.0	6.810949827819188E-5	0.0	0.04066137047208056	2.3838324397367159E-4
23	3.405474913909594E-5	6.810949827819188E-5	0.0	0.060140686979643435	2.724379931127675E-4
24	3.405474913909594E-5	6.810949827819188E-5	0.0	0.09130078244191622	2.724379931127675E-4
25	3.405474913909594E-5	6.810949827819188E-5	0.0	0.10529728433808465	2.724379931127675E-4
26	3.405474913909594E-5	6.810949827819188E-5	0.0	0.1268879952922715	2.724379931127675E-4
27	3.405474913909594E-5	6.810949827819188E-5	0.0	0.16131734667189748	2.724379931127675E-4
28	3.405474913909594E-5	6.810949827819188E-5	0.0	0.2401200361797655	2.724379931127675E-4
29	3.405474913909594E-5	6.810949827819188E-5	0.0	0.3232476788282987	2.724379931127675E-4
30	3.405474913909594E-5	6.810949827819188E-5	0.0	0.4514297545878558	2.724379931127675E-4
31	3.405474913909594E-5	6.810949827819188E-5	0.0	0.7544489124275315	2.724379931127675E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACCG	85	2.7254513E-5	17.411764	5
GTATAGT	235	0.0	17.319149	1
CGCTTCG	500	0.0	16.279999	32
CACTCTA	570	0.0	15.578947	9
CTTATAC	2785	0.0	14.94614	37
CTTAGCG	100	1.09428336E-4	14.8	3
AAGACGG	480	0.0	14.645834	5
CGAACGA	330	0.0	14.575757	16
GTAAACG	560	0.0	14.535715	27
CCGATAA	335	0.0	14.35821	9
GTATAGG	290	0.0	14.034483	1
TAACGAA	370	0.0	14.0	13
ACGAACG	345	0.0	13.942028	15
TACCGTT	120	3.3037944E-5	13.875001	7
CGATAAC	360	0.0	13.875	10
TACCGCA	660	0.0	13.734848	28
TAAACGC	615	0.0	13.536585	28
CGAGCCG	670	0.0	13.529852	15
CCTTATA	235	5.638867E-11	13.382979	2
AGTACTC	755	0.0	13.231789	5
>>END_MODULE