##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727046.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4686387
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07735084618492	33.0	31.0	34.0	30.0	34.0
2	32.2326786072085	33.0	31.0	34.0	30.0	34.0
3	32.336839659208685	34.0	31.0	34.0	30.0	34.0
4	35.916151184270525	37.0	35.0	37.0	35.0	37.0
5	35.7028824124	37.0	35.0	37.0	33.0	37.0
6	35.74640421288297	37.0	35.0	37.0	35.0	37.0
7	35.70294664098377	37.0	35.0	37.0	33.0	37.0
8	35.72044690291263	37.0	35.0	37.0	33.0	37.0
9	37.47099460629265	39.0	37.0	39.0	35.0	39.0
10	37.31800318667664	39.0	37.0	39.0	34.0	39.0
11	37.39325689491713	39.0	37.0	39.0	34.0	39.0
12	37.325380725066026	39.0	37.0	39.0	34.0	39.0
13	37.35600516986754	39.0	37.0	39.0	34.0	39.0
14	38.53587806555455	40.0	38.0	41.0	34.0	41.0
15	38.43123753970809	40.0	38.0	41.0	34.0	41.0
16	38.371426858259895	40.0	38.0	41.0	34.0	41.0
17	38.439737691317426	40.0	38.0	41.0	34.0	41.0
18	38.45407944328968	40.0	38.0	41.0	34.0	41.0
19	38.51555814745986	40.0	38.0	41.0	34.0	41.0
20	38.4892470468188	40.0	38.0	41.0	34.0	41.0
21	38.38069497888245	40.0	38.0	41.0	34.0	41.0
22	38.33957716253481	40.0	38.0	41.0	34.0	41.0
23	38.235699271101595	40.0	38.0	41.0	34.0	41.0
24	38.14256270342163	40.0	38.0	41.0	33.0	41.0
25	38.09476874189007	40.0	37.0	41.0	33.0	41.0
26	37.867970357548366	40.0	37.0	41.0	33.0	41.0
27	37.74344500358165	40.0	37.0	41.0	33.0	41.0
28	37.62210077827546	40.0	37.0	41.0	33.0	41.0
29	37.47734555426174	39.0	36.0	41.0	32.0	41.0
30	37.354139126794266	39.0	36.0	41.0	32.0	41.0
31	37.223014445883365	39.0	36.0	41.0	32.0	41.0
32	37.01819994806233	39.0	35.0	41.0	31.0	41.0
33	36.820839166718415	39.0	35.0	40.0	31.0	41.0
34	36.69826563619266	39.0	35.0	40.0	31.0	41.0
35	36.54861815722859	39.0	35.0	40.0	30.0	41.0
36	36.30773813600968	39.0	35.0	40.0	30.0	41.0
37	36.19897908559408	39.0	35.0	40.0	30.0	41.0
38	36.074147098820475	39.0	35.0	40.0	30.0	41.0
39	35.86663500048118	39.0	35.0	40.0	28.0	41.0
40	35.66224193605863	38.0	35.0	40.0	27.0	41.0
41	35.481534708934625	38.0	35.0	40.0	26.0	41.0
42	35.163745119641206	38.0	35.0	40.0	24.0	41.0
43	33.93826395472674	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	15.0
10	46.0
11	43.0
12	56.0
13	33.0
14	38.0
15	65.0
16	118.0
17	176.0
18	332.0
19	533.0
20	1214.0
21	2272.0
22	4064.0
23	6823.0
24	11280.0
25	17501.0
26	26051.0
27	38006.0
28	52514.0
29	69559.0
30	87337.0
31	108647.0
32	136229.0
33	174027.0
34	230542.0
35	306537.0
36	447926.0
37	735582.0
38	1226220.0
39	1002597.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.76523449727903	18.270428797280296	12.378000365740174	23.586336339700498
2	17.487565580904864	22.70922141086513	35.43740625774184	24.36580675048817
3	20.997753706640104	25.703660410461193	29.280467020756074	24.018118862142625
4	14.515361194028578	15.567579032632176	33.333290656533485	36.58376911680576
5	13.903631945035697	38.90414940123383	32.28916007150071	14.903058582229765
6	33.22932997210858	33.618008926706224	17.023604751378834	16.129056349806365
7	27.60561601079894	32.07925423145805	22.138781965723275	18.176347792019737
8	28.346293210526575	32.893762294919306	20.91863091972558	17.84131357482854
9	28.74177911469966	13.403502527640162	20.48315685409677	37.371561503563406
10	17.94427135445707	27.349640565322495	31.637613368251493	23.068474711968943
11	34.293539991468904	22.563693523390192	21.403567396375927	21.73919908876497
12	21.80026958934463	27.257181278456088	29.80808029725245	21.134468834946837
13	29.94223481756842	19.505879476022788	26.981126398652094	23.5707593077567
14	22.702478476489457	21.51264929678236	27.46399305050991	28.320879176218266
15	25.92391537446651	26.745187710703362	24.58175135770904	22.749145557121082
16	22.652311898270458	25.981721099857953	27.78910491173691	23.576862090134682
17	22.812157852093733	26.865386917469685	27.067354872740985	23.2551003576956
18	22.726462838002924	24.209204233453193	29.712121512798667	23.352211415745224
19	23.03181534090121	25.473056322493214	29.98666136620813	21.508466970397453
20	22.90971701654174	24.55305120981259	29.907218503294757	22.63001327035091
21	23.682039063355205	25.122786487756986	29.945051486358253	21.250122962529556
22	23.591606924481482	24.919900981288997	28.487574756416826	23.00091733781269
23	22.04137217007473	25.600083817234896	29.65580947540184	22.70273453728853
24	21.858864835533215	26.785752008957008	29.194814683465108	22.16056847204467
25	22.570031028167328	25.793601766136685	28.563496783342902	23.072870422353084
26	22.387651724025353	26.351366201724268	29.257421548839226	22.003560525411153
27	22.845829847172247	26.259355021256248	28.388543242374137	22.50627188919737
28	21.724134178419323	26.910965739705233	29.392237559552807	21.972662522322633
29	21.926038118490855	26.642037885475528	29.676785122526162	21.75513887350746
30	22.004968859806073	27.375097276430647	29.450085961744087	21.16984790201919
31	22.63248852474198	26.780353393776483	28.52039321549842	22.066764865983114
32	21.115413643815586	27.417731399476825	28.93644933719729	22.530405619510297
33	21.18245889637369	26.840613035159066	29.403013451513928	22.573914616953317
34	21.800162897345015	26.954410721948484	29.412210301880748	21.833216078825757
35	21.836544869213746	27.17504977715242	29.427168520226775	21.561236833407058
36	21.268089895264733	27.423620797855573	28.899469890130714	22.40881941674898
37	21.34390523019119	27.30854280707078	29.282302123149456	22.06524983958858
38	21.409904901153062	26.686805848514005	29.36695155564404	22.536337694688893
39	21.338357246211206	26.39899351035243	29.449296440946938	22.813352802489423
40	21.515658011171507	26.62471110473804	29.706317468019606	22.153313416070844
41	20.344713315396273	27.16679181638221	29.855707605880603	22.632787262340905
42	21.17851129238793	27.317184859039596	29.513076918316816	21.991226930255653
43	20.38713405444322	26.366431112069915	29.456722204120144	23.789712629366715
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1007.0
1	1543.0
2	2079.0
3	7617.0
4	13155.0
5	13155.0
6	18762.5
7	24370.0
8	22921.0
9	21472.0
10	29047.5
11	36623.0
12	36623.0
13	56691.0
14	76759.0
15	97698.5
16	118638.0
17	102506.5
18	86375.0
19	86375.0
20	88034.0
21	89693.0
22	70546.0
23	51399.0
24	58037.0
25	64675.0
26	64675.0
27	71264.5
28	77854.0
29	82907.5
30	87961.0
31	93759.5
32	99558.0
33	99558.0
34	111437.5
35	123317.0
36	140673.0
37	158029.0
38	178380.5
39	198732.0
40	198732.0
41	218798.5
42	238865.0
43	256044.0
44	273223.0
45	304962.5
46	336702.0
47	336702.0
48	376877.0
49	417052.0
50	414471.0
51	411890.0
52	378150.0
53	344410.0
54	344410.0
55	326666.0
56	308922.0
57	293006.0
58	277090.0
59	255663.0
60	234236.0
61	234236.0
62	198640.5
63	163045.0
64	142640.5
65	122236.0
66	103597.0
67	84958.0
68	84958.0
69	69344.5
70	53731.0
71	45618.0
72	37505.0
73	28424.0
74	19343.0
75	19343.0
76	15374.5
77	11406.0
78	9570.5
79	7735.0
80	6301.0
81	4867.0
82	4867.0
83	4171.5
84	3476.0
85	2921.5
86	2367.0
87	1630.5
88	894.0
89	894.0
90	648.5
91	403.0
92	301.0
93	199.0
94	153.5
95	108.0
96	108.0
97	64.0
98	20.0
99	14.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4686387.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.70236335876605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.93549136292847	39.562189443322175
2	8.865219797808479	8.45783872100773
3	2.8722119921736815	4.110339002822228
4	1.3931852682578878	2.658329195700709
5	0.8269495765012334	1.9723724588819769
6	0.5652926328039879	1.6179476744429606
7	0.38502307422709325	1.2856557411802971
8	0.3097762368876755	1.1821646889541668
9	0.22094952413806163	0.9485833035942239
>10	1.4171605841253476	12.549106053123607
>50	0.10958212870398652	3.6292792854139133
>100	0.07903937765408889	7.69196876973494
>500	0.013085890652668169	4.277043986815493
>1k	0.006453925347788896	5.198055967685064
>5k	3.5607863987800455E-4	1.2019996178506778
>10k+	2.2254914992375286E-4	3.657126089469938
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	53000	1.130935195919586	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	42022	0.8966822415647705	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	38364	0.8186263746463961	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28480	0.6077176298073548	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10378	0.22144991440100872	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9843	0.2100338704421978	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7402	0.1579468362301278	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	7175	0.1531030194476043	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7163	0.15284695864852818	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6924	0.14774708106692852	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	6336	0.13520010191219803	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	6138	0.13097509872744184	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	5631	0.12015652996647523	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.1338399923011053E-5
2	0.0	0.0	0.0	2.1338399923011053E-5	4.2676799846022107E-5
3	0.0	0.0	0.0	2.1338399923011053E-5	4.2676799846022107E-5
4	0.0	0.0	0.0	2.1338399923011053E-5	4.2676799846022107E-5
5	0.0	2.1338399923011053E-5	0.0	2.1338399923011053E-5	4.2676799846022107E-5
6	0.0	2.1338399923011053E-5	0.0	2.1338399923011053E-5	6.401519976903316E-5
7	0.0	2.1338399923011053E-5	0.0	4.2676799846022107E-5	6.401519976903316E-5
8	2.1338399923011053E-5	2.1338399923011053E-5	0.0	6.401519976903316E-5	8.535359969204421E-5
9	2.1338399923011053E-5	2.1338399923011053E-5	0.0	1.0669199961505527E-4	1.0669199961505527E-4
10	4.2676799846022107E-5	2.1338399923011053E-5	0.0	1.4936879946107737E-4	1.0669199961505527E-4
11	4.2676799846022107E-5	2.1338399923011053E-5	0.0	3.4141439876817685E-4	1.0669199961505527E-4
12	6.401519976903316E-5	2.1338399923011053E-5	0.0	5.547983979982873E-4	1.0669199961505527E-4
13	6.401519976903316E-5	2.1338399923011053E-5	0.0	7.468439973053869E-4	1.2803039953806632E-4
14	8.535359969204421E-5	2.1338399923011053E-5	0.0	0.0013869959949957184	1.4936879946107737E-4
15	1.0669199961505527E-4	2.1338399923011053E-5	0.0	0.002368562391454227	1.4936879946107737E-4
16	1.0669199961505527E-4	2.1338399923011053E-5	0.0	0.00527058478098373	1.4936879946107737E-4
17	1.0669199961505527E-4	2.1338399923011053E-5	0.0	0.008236622370282266	1.4936879946107737E-4
18	1.0669199961505527E-4	2.1338399923011053E-5	0.0	0.009602279965354973	1.4936879946107737E-4
19	1.2803039953806632E-4	2.1338399923011053E-5	0.0	0.012653671154345554	1.4936879946107737E-4
20	1.2803039953806632E-4	2.1338399923011053E-5	0.0	0.015897107942643235	1.4936879946107737E-4
21	1.2803039953806632E-4	2.1338399923011053E-5	0.0	0.022618703918391717	1.7070719938408843E-4
22	1.2803039953806632E-4	2.1338399923011053E-5	0.0	0.03298916628097509	1.7070719938408843E-4
23	1.2803039953806632E-4	2.1338399923011053E-5	0.0	0.051297513414918575	2.3472239915312159E-4
24	1.2803039953806632E-4	2.1338399923011053E-5	0.0	0.07993364611159941	2.5606079907613264E-4
25	1.2803039953806632E-4	2.1338399923011053E-5	0.0	0.09220322606733077	2.5606079907613264E-4
26	1.2803039953806632E-4	2.1338399923011053E-5	0.0	0.1113864475981177	2.7739919899914367E-4
27	1.2803039953806632E-4	4.2676799846022107E-5	0.0	0.14458899787832288	2.7739919899914367E-4
28	1.2803039953806632E-4	4.2676799846022107E-5	0.0	0.21707554241679144	2.7739919899914367E-4
29	1.2803039953806632E-4	4.2676799846022107E-5	0.0	0.30294126370698793	3.2007599884516577E-4
30	1.2803039953806632E-4	4.2676799846022107E-5	0.0	0.43624224802603795	3.2007599884516577E-4
31	1.2803039953806632E-4	4.2676799846022107E-5	0.0	0.7635733028450276	3.2007599884516577E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	565	0.0	15.061946	28
AAGACGG	1025	0.0	14.619512	5
ACGGACC	1070	0.0	14.523365	8
CAAGACG	1035	0.0	14.299518	4
CTTATAC	4005	0.0	14.042448	37
CGGACCA	1095	0.0	14.022832	9
CTAGCGG	615	0.0	13.837399	29
GACGGAC	1140	0.0	13.631578	7
TCTTATA	6240	0.0	13.430289	37
AGACGGA	1240	0.0	13.42742	6
TACGGAT	1100	0.0	13.118182	27
TCGTTAC	685	0.0	12.963504	23
TATACTG	570	0.0	12.657894	5
GTCCTAT	955	0.0	12.591622	1
GAGCGAA	1195	0.0	12.539748	16
CGCAATA	725	0.0	12.5034485	36
CGGTCCA	760	0.0	12.414474	10
ACGGATG	1195	0.0	12.384936	28
CATTACG	540	0.0	12.333334	24
CGTTACG	645	0.0	12.333334	24
>>END_MODULE