FastQCFastQC Report
Thu 9 Feb 2017
SRR2727045.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727045.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975717
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT133411.3673021993057413No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT90980.9324425012580492No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69250.7097344824370181No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT68100.6979482780355369No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24270.24874015723821558No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21150.21676367225332754No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC20490.2099994158142166No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19220.1969833466056244No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18480.18939918029510608No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT17260.17689555475614344No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA11550.11837448768444128No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11410.11693964540947835No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10510.10771565935614527No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG358.8684296E-426.4285725
GACTGCG502.7016966E-422.27
TAATAGT1409.458745E-1118.54
CCGCTCA500.007034138318.59
ATACCGT1800.017.4722216
GACGGAC4450.017.4606747
CAAGACG4300.017.2093034
GTAGTAC650.001579703117.0769233
ACGGACC4600.016.8913048
AAGACGG4500.016.8555565
TATACTG904.444849E-516.4444455
TACCGTC1801.0913936E-1116.4444457
TACGACG1151.2416476E-616.0869565
ACACCGC700.002592116815.8571436
CGGACCA4800.015.8020839
CCGTCGT1902.7284841E-1115.5789489
ACCGTCG1902.7284841E-1115.5789488
TAGAGTG1557.201379E-915.5161295
CGCGTAA3350.015.46268710
TTATACT1201.9346735E-615.4166664
Produced by FastQC (version 0.11.5)