##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727045.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	975717
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.591523976726858	31.0	30.0	31.0	25.0	34.0
2	29.74812163772897	31.0	30.0	31.0	26.0	34.0
3	29.801661752331874	31.0	30.0	31.0	26.0	34.0
4	33.27592221925005	35.0	33.0	37.0	28.0	37.0
5	31.5132656292757	35.0	30.0	35.0	19.0	37.0
6	31.87957573763704	35.0	30.0	35.0	23.0	37.0
7	31.806950171002452	35.0	30.0	35.0	23.0	37.0
8	31.793746547410777	35.0	30.0	35.0	23.0	37.0
9	32.906602016773306	35.0	31.0	38.0	23.0	39.0
10	32.363560335630105	35.0	30.0	37.0	19.0	39.0
11	32.86724019362172	35.0	31.0	38.0	23.0	39.0
12	32.545140650414005	35.0	30.0	37.0	23.0	39.0
13	32.80820873265506	35.0	30.0	38.0	23.0	39.0
14	33.193050853884884	36.0	31.0	39.0	22.0	40.0
15	33.16208183315449	36.0	31.0	39.0	22.0	40.0
16	32.99690586512278	36.0	31.0	38.0	19.0	40.0
17	33.060452979706206	36.0	31.0	38.0	21.0	40.0
18	33.042107496333465	36.0	31.0	38.0	21.0	40.0
19	33.086584532195296	36.0	30.0	39.0	21.0	40.0
20	32.992964148415986	36.0	30.0	39.0	19.0	40.0
21	32.802080931253634	36.0	30.0	39.0	19.0	40.0
22	32.72412697534224	36.0	30.0	39.0	19.0	40.0
23	32.50864338737564	35.0	30.0	38.0	19.0	40.0
24	32.28772687162364	35.0	30.0	38.0	18.0	40.0
25	32.22202031941639	35.0	29.0	38.0	18.0	40.0
26	31.78500118374488	35.0	29.0	38.0	17.0	40.0
27	31.519091088912052	34.0	27.0	38.0	17.0	40.0
28	31.285946642315345	34.0	27.0	38.0	16.0	40.0
29	31.023183976501382	34.0	27.0	38.0	16.0	40.0
30	30.794289737700584	34.0	27.0	38.0	15.0	40.0
31	30.595095709104175	34.0	26.0	38.0	15.0	40.0
32	30.266330298641922	33.0	25.0	38.0	15.0	40.0
33	29.983661246037528	33.0	25.0	38.0	14.0	40.0
34	29.771019670662703	33.0	25.0	38.0	12.0	40.0
35	29.476183155566623	33.0	24.0	37.0	10.0	40.0
36	29.090079398022173	33.0	24.0	37.0	9.0	40.0
37	28.9122040509697	33.0	23.0	37.0	9.0	40.0
38	28.686245089508535	33.0	23.0	37.0	9.0	40.0
39	28.42106881401062	32.0	22.0	37.0	9.0	40.0
40	28.13392920283238	32.0	21.0	37.0	9.0	39.0
41	27.868803146814088	31.0	21.0	37.0	8.0	39.0
42	27.46710265374079	31.0	19.0	37.0	8.0	39.0
43	26.365954472454614	30.0	17.0	35.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	8.0
10	31.0
11	71.0
12	161.0
13	274.0
14	581.0
15	1167.0
16	2038.0
17	3484.0
18	5423.0
19	8173.0
20	11492.0
21	15675.0
22	21049.0
23	26911.0
24	32761.0
25	39135.0
26	45602.0
27	51352.0
28	56780.0
29	61212.0
30	63679.0
31	65760.0
32	66700.0
33	67128.0
34	66202.0
35	65745.0
36	63774.0
37	59831.0
38	52688.0
39	20828.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.21500701535384	22.749526758271095	14.550325555463317	20.48514067091175
2	19.210898242010746	26.804288538582394	31.43995646278583	22.54485675662103
3	23.834165029409142	26.80418604984847	28.715395960099087	20.646252960643302
4	18.256113196756846	17.31126955869376	31.69628078633456	32.73633645821483
5	14.142932838107772	40.87373695446528	30.771012496451327	14.212317710975622
6	33.100991373523264	34.96731121831433	15.484305387730254	16.447392020432154
7	26.658344581471884	32.311828122293655	22.998471892977165	18.031355403257297
8	28.36765168588843	33.87539624706754	20.87131822034463	16.885633846699402
9	27.345121587509492	13.330812110478757	22.042456982916153	37.2816093190956
10	17.969657185433892	28.555103580238946	32.34585438195706	21.1293848523701
11	34.39409172946664	21.923980006497786	22.341314131044147	21.34061413299143
12	20.664905910217822	27.907682248028888	31.171128513698132	20.256283328055165
13	30.068452225389127	19.20987335467149	28.735483751948564	21.986190667990822
14	22.284125417513483	21.07691062060003	29.92271324574646	26.716250716140028
15	25.18312174534214	27.043804709767276	26.169063365709523	21.604010179181053
16	22.343773860658366	25.31266750502451	29.5492442993204	22.794314334996727
17	22.092471485072004	26.9236879136061	29.463153762822618	21.520686838499277
18	21.998386827328005	23.346933588325303	32.32176952948447	22.33291005486222
19	22.63484186500799	24.6009857366429	32.520700162034686	20.24347223631442
20	22.84894083017924	23.455469157552855	32.52367233531854	21.171917676949363
21	23.14113621060205	24.20476429128528	32.487083857306985	20.167015640805687
22	22.851093093591686	24.519609681905717	31.701200245563005	20.92809697893959
23	21.723102088002978	24.719360224327342	32.47919222479469	21.078345462874992
24	21.986088179256896	25.616956556050575	31.84991139848952	20.54704386620301
25	22.116351360076745	24.853722954504224	31.677115393090418	21.352810292328616
26	22.153247304290076	25.342799192798733	32.03726080410611	20.466692698805083
27	22.242514991539554	24.90363496792615	31.594509473546122	21.259340566988175
28	21.947039971631117	25.134234619259477	32.596746802607726	20.32197860650168
29	22.103335290868152	25.021394523207036	32.21497626873366	20.660293917191154
30	21.92316009662638	25.77017721326983	32.18217987387737	20.124482816226426
31	22.592718995364436	25.340544440652362	31.54705718973842	20.519679374244788
32	21.44443522045839	25.901465281428937	31.740248453188784	20.913851044923888
33	21.58043777037809	25.672710427306278	31.852678594305523	20.89417320801011
34	22.05352576618015	25.582417852717544	31.54070288823501	20.823353492867298
35	22.388561437383995	25.85421797508909	31.464963713863753	20.292256873663163
36	21.65494707994224	26.219487822801078	30.713618805452807	21.411946291803872
37	22.221607289818667	26.092914236402564	30.829533563523032	20.85594491025574
38	22.23482833649511	25.51129067137295	30.83260822554081	21.42127276659113
39	22.710581039379246	25.43985602382658	30.234483974349118	21.615078962445054
40	22.56894160909362	25.926472532506867	30.46446869327889	21.040117165120627
41	21.74482969959527	26.071801557213824	30.15505520555653	22.028313537634375
42	22.589541844612732	26.43717389365974	29.45433973170499	21.518944530022537
43	22.46235332581066	25.642783716999908	29.355848058402177	22.53901489878725
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	393.0
1	464.0
2	535.0
3	1433.5
4	2332.0
5	2332.0
6	3887.5
7	5443.0
8	6011.0
9	6579.0
10	8308.5
11	10038.0
12	10038.0
13	15414.5
14	20791.0
15	27320.5
16	33850.0
17	28803.5
18	23757.0
19	23757.0
20	23504.0
21	23251.0
22	18694.0
23	14137.0
24	13730.0
25	13323.0
26	13323.0
27	14298.5
28	15274.0
29	16232.5
30	17191.0
31	18853.5
32	20516.0
33	20516.0
34	23265.0
35	26014.0
36	29846.0
37	33678.0
38	37559.5
39	41441.0
40	41441.0
41	45183.5
42	48926.0
43	52430.5
44	55935.0
45	61041.5
46	66148.0
47	66148.0
48	74113.0
49	82078.0
50	80798.5
51	79519.0
52	73832.0
53	68145.0
54	68145.0
55	65046.5
56	61948.0
57	58687.0
58	55426.0
59	51048.5
60	46671.0
61	46671.0
62	39718.5
63	32766.0
64	28329.0
65	23892.0
66	20044.0
67	16196.0
68	16196.0
69	13498.5
70	10801.0
71	9116.0
72	7431.0
73	5873.0
74	4315.0
75	4315.0
76	3459.5
77	2604.0
78	2114.0
79	1624.0
80	1351.5
81	1079.0
82	1079.0
83	916.5
84	754.0
85	654.0
86	554.0
87	385.0
88	216.0
89	216.0
90	154.0
91	92.0
92	67.0
93	42.0
94	26.5
95	11.0
96	11.0
97	6.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	975717.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.52935022795357
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.42654608413247	61.06445900419567
2	5.307707274953894	7.168520469556372
3	1.6532209589271665	3.349228114187577
4	0.7767564329230369	2.098154288027028
5	0.43299997862190986	1.462010360252768
6	0.29561373134454827	1.1977561917694908
7	0.18922412369501423	0.8944727484404759
8	0.14393218556591325	0.7775717574524289
9	0.10681023384429354	0.6491543120280827
>10	0.561966224268645	7.123870241171994
>50	0.05952561411066249	2.7566845072104056
>100	0.041446259547394046	5.118799506504781
>500	0.002277266820565917	1.0483855317923607
>1k	0.0013663600923395501	1.5823178464388121
>5k	4.554533641131834E-4	2.340874911736258
>10k+	1.518177880377278E-4	1.3677402092354667
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13341	1.3673021993057413	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9098	0.9324425012580492	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6925	0.7097344824370181	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6810	0.6979482780355369	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2427	0.24874015723821558	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2115	0.21676367225332754	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	2049	0.2099994158142166	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1922	0.1969833466056244	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1848	0.18939918029510608	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1726	0.17689555475614344	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	1155	0.11837448768444128	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1141	0.11693964540947835	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	1051	0.10771565935614527	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.024887339259232E-4	0.0
11	0.0	0.0	0.0	1.024887339259232E-4	1.024887339259232E-4
12	0.0	0.0	0.0	2.049774678518464E-4	1.024887339259232E-4
13	0.0	0.0	0.0	2.049774678518464E-4	1.024887339259232E-4
14	0.0	1.024887339259232E-4	0.0	2.049774678518464E-4	2.049774678518464E-4
15	0.0	1.024887339259232E-4	0.0	4.099549357036928E-4	2.049774678518464E-4
16	0.0	1.024887339259232E-4	0.0	0.0015373310088888478	2.049774678518464E-4
17	0.0	1.024887339259232E-4	0.0	0.00225475214637031	2.049774678518464E-4
18	0.0	1.024887339259232E-4	0.0	0.0029721732838517726	2.049774678518464E-4
19	0.0	1.024887339259232E-4	0.0	0.003997060623111005	2.049774678518464E-4
20	0.0	1.024887339259232E-4	0.0	0.00450950429274062	2.049774678518464E-4
21	0.0	1.024887339259232E-4	0.0	0.007481677576592393	2.049774678518464E-4
22	0.0	1.024887339259232E-4	0.0	0.01219615933718486	2.049774678518464E-4
23	1.024887339259232E-4	1.024887339259232E-4	0.0	0.02060023551911056	2.049774678518464E-4
24	1.024887339259232E-4	1.024887339259232E-4	0.0	0.03515363573659166	2.049774678518464E-4
25	1.024887339259232E-4	1.024887339259232E-4	0.0	0.03945816256148043	2.049774678518464E-4
26	1.024887339259232E-4	1.024887339259232E-4	0.0	0.04837468241303575	2.049774678518464E-4
27	1.024887339259232E-4	1.024887339259232E-4	0.0	0.06046835301629468	2.049774678518464E-4
28	1.024887339259232E-4	1.024887339259232E-4	0.0	0.0879353337084421	2.049774678518464E-4
29	1.024887339259232E-4	1.024887339259232E-4	0.0	0.1232939469128856	2.049774678518464E-4
30	1.024887339259232E-4	1.024887339259232E-4	0.0	0.1821224801863655	2.049774678518464E-4
31	1.024887339259232E-4	1.024887339259232E-4	0.0	0.3267340837558431	2.049774678518464E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	35	8.8684296E-4	26.428572	5
GACTGCG	50	2.7016966E-4	22.2	7
TAATAGT	140	9.458745E-11	18.5	4
CCGCTCA	50	0.0070341383	18.5	9
ATACCGT	180	0.0	17.472221	6
GACGGAC	445	0.0	17.460674	7
CAAGACG	430	0.0	17.209303	4
GTAGTAC	65	0.0015797031	17.076923	3
ACGGACC	460	0.0	16.891304	8
AAGACGG	450	0.0	16.855556	5
TATACTG	90	4.444849E-5	16.444445	5
TACCGTC	180	1.0913936E-11	16.444445	7
TACGACG	115	1.2416476E-6	16.086956	5
ACACCGC	70	0.0025921168	15.857143	6
CGGACCA	480	0.0	15.802083	9
CCGTCGT	190	2.7284841E-11	15.578948	9
ACCGTCG	190	2.7284841E-11	15.578948	8
TAGAGTG	155	7.201379E-9	15.516129	5
CGCGTAA	335	0.0	15.462687	10
TTATACT	120	1.9346735E-6	15.416666	4
>>END_MODULE