FastQCFastQC Report
Wed 25 May 2016
SRR2558182_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2558182_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26858691
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1253180.46658267895483063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT705790.2627790014040521No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT619070.2304915008702397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA249550.031.2713741
GTATCAA680350.026.657131
TATCAAC960900.018.7002872
ATCAACG973950.018.3667893
TCAACGC981000.018.1608224
CAACGCA989950.017.9630625
AACGCAG1025900.017.601656
ACGCAGA1164400.015.41325957
CGCAGAG1169400.015.2927148
AGAGTAC1222100.014.52854911
TCTAGAC51650.014.5247943
GCAGAGT1226800.014.4335479
CAGAGTA1230050.014.41297210
GTATTAG85250.014.2240421
GAGTACT716150.013.87544512
AGTACTT726800.013.73245713
ACCGTCG14100.012.9499928
GTACTTT773800.012.92982314
GTACATG969100.012.8487281
CTAGACA74350.012.6941334
Produced by FastQC (version 0.11.5)