##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2558182_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26858691
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.949127267594687	33.0	32.0	34.0	25.0	34.0
2	31.14036916393282	33.0	32.0	34.0	25.0	34.0
3	31.186834161054236	33.0	32.0	34.0	25.0	34.0
4	31.00567175816573	33.0	32.0	34.0	25.0	34.0
5	30.958584206505076	33.0	32.0	34.0	25.0	34.0
6	34.256702606988554	38.0	34.0	38.0	26.0	38.0
7	34.4285997035373	38.0	34.0	38.0	26.0	38.0
8	34.55431163789777	38.0	35.0	38.0	26.0	38.0
9	34.70720397356669	38.0	35.0	38.0	26.0	38.0
10	34.602466292940335	38.0	35.0	38.0	26.0	38.0
11	34.62620207366025	38.0	35.0	38.0	26.0	38.0
12	34.609635815833315	38.0	35.0	38.0	26.0	38.0
13	34.59965830054786	38.0	35.0	38.0	26.0	38.0
14	34.56523815698986	38.0	35.0	38.0	26.0	38.0
15	34.568501123156004	38.0	35.0	38.0	26.0	38.0
16	34.564327166949425	38.0	35.0	38.0	26.0	38.0
17	34.5709733955389	38.0	35.0	38.0	26.0	38.0
18	34.49238624473546	38.0	35.0	38.0	25.0	38.0
19	34.51413719305978	38.0	35.0	38.0	25.0	38.0
20	34.40280265333854	38.0	35.0	38.0	24.0	38.0
21	34.47695459171856	38.0	35.0	38.0	25.0	38.0
22	34.500357072502155	38.0	35.0	38.0	25.0	38.0
23	34.49847049508109	38.0	35.0	38.0	25.0	38.0
24	34.48639120201353	38.0	35.0	38.0	25.0	38.0
25	34.48249920295818	38.0	35.0	38.0	25.0	38.0
26	34.46848314387324	38.0	35.0	38.0	24.0	38.0
27	34.453686667008455	38.0	35.0	38.0	24.0	38.0
28	34.450746501383854	38.0	35.0	38.0	24.0	38.0
29	34.39717445649157	38.0	35.0	38.0	24.0	38.0
30	34.39227607927728	38.0	35.0	38.0	24.0	38.0
31	34.360744460703614	38.0	35.0	38.0	24.0	38.0
32	34.35566379612469	38.0	35.0	38.0	24.0	38.0
33	34.35094934447848	38.0	35.0	38.0	24.0	38.0
34	34.30865692598348	38.0	35.0	38.0	16.0	38.0
35	34.337232294753306	38.0	35.0	38.0	24.0	38.0
36	34.32142076469773	38.0	35.0	38.0	16.0	38.0
37	34.32196341958735	38.0	35.0	38.0	16.0	38.0
38	34.2942821003451	38.0	35.0	38.0	16.0	38.0
39	34.279030240155784	38.0	34.0	38.0	16.0	38.0
40	34.260543523882085	38.0	34.0	38.0	16.0	38.0
41	34.267188039804324	38.0	34.0	38.0	16.0	38.0
42	34.23064463566002	38.0	34.0	38.0	16.0	38.0
43	34.200604340695534	38.0	34.0	38.0	16.0	38.0
44	34.16910660314756	38.0	34.0	38.0	16.0	38.0
45	34.11773648983861	38.0	34.0	38.0	16.0	38.0
46	34.06579993045826	38.0	34.0	38.0	16.0	38.0
47	34.057008958478285	38.0	34.0	38.0	16.0	38.0
48	33.9500476773049	38.0	34.0	38.0	16.0	38.0
49	33.95320672924827	38.0	34.0	38.0	16.0	38.0
50	33.60438905976468	38.0	33.0	38.0	16.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	4.0
8	10.0
9	15.0
10	18.0
11	25.0
12	25.0
13	34.0
14	32.0
15	101.0
16	485.0
17	7088.0
18	52076.0
19	163613.0
20	280903.0
21	350263.0
22	384026.0
23	408612.0
24	435943.0
25	467792.0
26	506777.0
27	550116.0
28	605140.0
29	673365.0
30	756075.0
31	860435.0
32	1001798.0
33	1205241.0
34	1541961.0
35	2185676.0
36	4121881.0
37	1.0299158E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.94474022855724	20.877103702822343	14.47117766765708	23.70697840096334
2	14.594763847203213	21.823164262637558	40.90327329553435	22.678798594624883
3	16.54733470227585	28.238538680668245	31.719047482885237	23.495079134170663
4	11.949704502334527	18.26092505854234	39.864295477038404	29.92507496208473
5	12.420105632600606	38.674423418114515	35.97670874617574	12.928762203109137
6	27.835014158669136	40.11658915255156	19.0610776077963	12.987319080983001
7	24.95585112661074	33.135752154200674	24.862973737051806	17.045422982136788
8	24.501181211321235	35.52419931506164	21.61333930110432	18.361280172512814
9	26.032354949344256	15.768054180730468	21.688454991484463	36.51113587844081
10	15.338607303322155	29.027078196425133	33.62900329786016	22.005311202392555
11	31.613430180222025	25.013732444801118	25.132446000020774	18.24039137495608
12	21.94222814858921	27.27877326735213	30.936201620157995	19.842796963900664
13	27.022690243292192	22.87178534408807	27.506456119239388	22.599068293380355
14	21.879134169786848	21.954477238395807	28.62791246979166	27.538476122025692
15	22.563112390385744	29.717871690647605	25.616737276808088	22.102278642158566
16	21.965669670407614	29.04376414257484	26.38395199971703	22.606614187300515
17	20.61526801390598	28.790058144132697	28.142327650773602	22.452346191187726
18	20.804099762261774	27.94569978933697	29.882339441249457	21.367861007151806
19	22.115772620324247	27.283258636379916	30.32119454163558	20.279774201660253
20	23.105435564267513	26.78263707722485	29.635394051247623	20.476533307260016
21	22.640199604689663	26.989309583040153	29.12342765919849	21.24706315307169
22	22.244598071145393	26.910109019092516	29.319529741302162	21.52576316845992
23	21.672235276272154	27.131910201325184	29.397798219531758	21.7980563028709
24	21.472996106492246	27.7501305431698	29.01507375205049	21.761799598287464
25	21.961320057935804	26.99368229276019	29.511911216928528	21.53308643237548
26	21.639568968190975	27.249190762587094	29.411044351426607	21.700195917795323
27	21.300287772243113	27.33266282908289	29.330950664099674	22.036098734574328
28	21.302488568704387	27.23122120580919	29.858553820097573	21.60773640538885
29	21.820655487506897	27.250612167999044	29.359780157546805	21.568952186947254
30	21.379459767956664	27.688736225305654	29.354859836861554	21.57694416987613
31	21.99785562845032	27.19599068945388	29.560034472582224	21.246119209513576
32	21.53108089913479	27.29133441005822	29.419655809459016	21.757928881347976
33	21.468418394181434	27.434434763234485	29.44822191758849	21.648924924995587
34	21.944714980506138	26.915547349825925	29.15216054330972	21.987577126358214
35	21.781749625969926	27.35785647089714	29.372644463182045	21.48774943995089
36	22.198179692083933	27.269959222847895	28.755470862902115	21.776390222166057
37	22.189053981683816	26.803404860636608	29.145556261888654	21.86198489579092
38	22.1234317802341	27.440090903390796	29.160901056999894	21.27557625937521
39	21.80010289161786	27.272548889435893	29.37467446107099	21.552673757875255
40	21.943002261475776	27.159487454426163	29.315885425461236	21.58162485863682
41	21.575866078579207	27.22740508313605	29.359001821610548	21.8377270166742
42	21.834812061200665	27.372183304716437	28.923304211542767	21.86970042254013
43	21.817909538704576	27.173458995389392	29.179417793161228	21.8292136727448
44	21.720244822687484	27.268721327003455	28.96765390917091	22.043379941138152
45	21.80854070197483	27.142493642276982	29.15160187961182	21.897363776136363
46	21.991215042684942	27.269546593321426	28.97558524433372	21.763653119659914
47	21.75039418428687	27.436380584761423	28.972314241166764	21.84091098978494
48	22.002069870875832	27.29341745168632	28.67263014268452	22.03188253475333
49	21.727378166973967	27.517242383310116	29.24165012323475	21.513729326481172
50	22.223544805899902	27.104883855971902	28.818829434760417	21.852741903367775
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7372.0
1	7108.5
2	6845.0
3	15465.5
4	24086.0
5	24808.0
6	25530.0
7	20384.5
8	15239.0
9	20246.0
10	25253.0
11	37182.0
12	49111.0
13	96595.0
14	144079.0
15	191600.0
16	239121.0
17	229041.5
18	218962.0
19	212472.0
20	205982.0
21	250164.0
22	294346.0
23	348090.0
24	401834.0
25	461612.0
26	521390.0
27	592729.0
28	664068.0
29	749355.5
30	834643.0
31	973352.5
32	1112062.0
33	1201757.0
34	1291452.0
35	1383930.5
36	1476409.0
37	1512346.5
38	1548284.0
39	1615174.5
40	1682065.0
41	1728713.0
42	1775361.0
43	1766146.5
44	1756932.0
45	1760538.0
46	1764144.0
47	1731752.5
48	1699361.0
49	1646071.0
50	1592781.0
51	1563380.0
52	1533979.0
53	1391433.5
54	1248888.0
55	1147237.0
56	1045586.0
57	959495.5
58	873405.0
59	808981.0
60	744557.0
61	664595.0
62	584633.0
63	516856.0
64	449079.0
65	385786.5
66	322494.0
67	275971.0
68	229448.0
69	195847.5
70	162247.0
71	136874.5
72	111502.0
73	90870.5
74	70239.0
75	57569.5
76	44900.0
77	34842.5
78	24785.0
79	19399.0
80	14013.0
81	11679.0
82	9345.0
83	7887.0
84	6429.0
85	4946.5
86	3464.0
87	3015.5
88	2567.0
89	1428.5
90	290.0
91	195.5
92	101.0
93	61.0
94	21.0
95	12.5
96	4.0
97	2.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.023590129541309363
2	0.010957347102284323
3	1.1169568911604815E-5
4	0.0029040879170172517
5	0.0032912996392862187
6	0.006132093332471042
7	0.002609955935678325
8	0.004065723083824152
9	0.0027142052455199697
10	0.0013626874072157874
11	0.002844516882822026
12	0.0034997982589695083
13	0.0016791585263779235
14	8.004857719983449E-4
15	0.0015451236994386659
16	0.001921165852796028
17	0.002259976109781374
18	5.882639626778535E-4
19	0.0014483207688714243
20	0.004069446273461354
21	0.0029971676579472915
22	0.0013701337864901906
23	0.002606232746041123
24	0.006236342642312687
25	0.002561554470394704
26	0.0037157432579272014
27	0.0011095105118860782
28	0.0031386488641609524
29	0.004750789977069248
30	0.005138001699338215
31	0.005231081440268254
32	0.007219264706533911
33	0.0034886286900579037
34	0.001835532491140391
35	0.004560907305571966
36	9.9781482277003E-4
37	0.0062326194526754865
38	0.004344962306614272
39	0.004855039286910892
40	0.005398624973942327
41	0.006284744107596309
42	0.005945933850610962
43	0.0046912189428740215
44	0.0032987460185606218
45	0.0029971676579472915
46	0.0018876571460612135
47	0.002587616797855115
48	0.0036375562755459672
49	0.0022339137823209625
50	0.0018131933533171813
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2.6858691E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.74541774023726
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.16504395450654	31.441219508701383
2	12.532659519713937	10.212968950538112
3	4.12227951028825	5.038920020661462
4	1.8345465312347895	2.9899745911625883
5	1.016841968157711	2.0715825384195483
6	0.6641660241535458	1.6237033261805238
7	0.4392216914817582	1.2527389909998492
8	0.31982003828723826	1.0424960849369758
9	0.24271009211223657	0.8900391683596088
>10	1.3882544213220902	10.398636459841693
>50	0.1266488427725053	3.6153109498047753
>100	0.11414155604056045	9.987867459555558
>500	0.018946891878001536	5.398781089458526
>1k	0.013990169447598242	10.694107729034025
>5k	5.350599870904045E-4	1.4738730120621144
>10k+	1.9372861601490036E-4	1.8677801202828785
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	125318	0.46658267895483063	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	70579	0.2627790014040521	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	61907	0.2304915008702397	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8615948186008022E-5	0.0	0.0	0.0	3.7231896372016046E-6
2	2.606232746041123E-5	0.0	0.0	0.0	3.7231896372016046E-6
3	2.606232746041123E-5	3.7231896372016046E-6	0.0	0.0	3.7231896372016046E-6
4	2.606232746041123E-5	3.7231896372016046E-6	0.0	7.446379274403209E-6	3.7231896372016046E-6
5	2.606232746041123E-5	3.7231896372016046E-6	0.0	1.1169568911604813E-5	3.7231896372016046E-6
6	2.606232746041123E-5	3.7231896372016046E-6	0.0	1.1169568911604813E-5	3.7231896372016046E-6
7	2.606232746041123E-5	3.7231896372016046E-6	0.0	1.1169568911604813E-5	3.7231896372016046E-6
8	2.606232746041123E-5	3.7231896372016046E-6	0.0	1.4892758548806418E-5	3.7231896372016046E-6
9	4.095508600921765E-5	3.7231896372016046E-6	0.0	2.9785517097612837E-5	3.7231896372016046E-6
10	1.3775801657645937E-4	3.7231896372016046E-6	0.0	4.467827564641925E-5	3.7231896372016046E-6
11	1.4520439585086257E-4	1.1169568911604813E-5	0.0	6.329422383242728E-5	3.7231896372016046E-6
12	1.712667233112738E-4	1.4892758548806418E-5	0.0	6.701741346962888E-5	3.7231896372016046E-6
13	1.712667233112738E-4	1.4892758548806418E-5	0.0	6.701741346962888E-5	3.7231896372016046E-6
14	1.749899129484754E-4	1.4892758548806418E-5	0.0	6.701741346962888E-5	3.7231896372016046E-6
15	1.9360586113448342E-4	1.4892758548806418E-5	0.0	6.701741346962888E-5	3.7231896372016046E-6
16	1.9732905077168503E-4	1.4892758548806418E-5	0.0	6.701741346962888E-5	1.4892758548806418E-5
17	2.2339137823209628E-4	1.4892758548806418E-5	0.0	7.074060310683049E-5	1.4892758548806418E-5
18	2.308377575064995E-4	1.4892758548806418E-5	0.0	7.818698238123369E-5	1.8615948186008022E-5
19	2.420073264181043E-4	1.4892758548806418E-5	0.0	7.818698238123369E-5	1.8615948186008022E-5
20	2.420073264181043E-4	1.4892758548806418E-5	0.0	8.56333616556369E-5	2.9785517097612837E-5
21	2.494537056925075E-4	1.4892758548806418E-5	0.0	9.307974093004011E-5	2.9785517097612837E-5
22	2.494537056925075E-4	1.4892758548806418E-5	0.0	9.680293056724171E-5	2.9785517097612837E-5
23	2.643464642413139E-4	1.8615948186008022E-5	0.0	1.0424930984164492E-4	3.350870673481444E-5
24	2.8668560206452357E-4	1.8615948186008022E-5	0.0	1.1169568911604814E-4	3.350870673481444E-5
25	3.0157836061333E-4	1.8615948186008022E-5	0.0	1.1914206839045135E-4	3.350870673481444E-5
26	3.0530155025053156E-4	2.2339137823209626E-5	3.7231896372016046E-6	1.2658844766485456E-4	3.350870673481444E-5
27	3.1274792952493477E-4	2.2339137823209626E-5	3.7231896372016046E-6	1.3031163730205616E-4	3.350870673481444E-5
28	3.164711191621364E-4	2.2339137823209626E-5	3.7231896372016046E-6	1.749899129484754E-4	3.7231896372016045E-5
29	3.276406880737412E-4	2.2339137823209626E-5	3.7231896372016046E-6	2.1594499895769307E-4	3.7231896372016045E-5
30	3.4625663625974923E-4	2.2339137823209626E-5	3.7231896372016046E-6	3.276406880737412E-4	3.7231896372016045E-5
31	3.6114939480855565E-4	2.606232746041123E-5	3.7231896372016046E-6	7.111292207055065E-4	3.7231896372016045E-5
32	3.6114939480855565E-4	2.9785517097612837E-5	3.7231896372016046E-6	0.001120680080797683	3.7231896372016045E-5
33	3.983812911805717E-4	2.9785517097612837E-5	3.7231896372016046E-6	0.001563739647624674	3.7231896372016045E-5
34	4.281668082781845E-4	3.350870673481444E-5	3.7231896372016046E-6	0.00201796878336327	3.7231896372016045E-5
35	4.281668082781845E-4	3.350870673481444E-5	3.7231896372016046E-6	0.0027067588662455667	3.7231896372016045E-5
36	4.3188999791538615E-4	3.350870673481444E-5	3.7231896372016046E-6	0.0035742620517135405	3.7231896372016045E-5
37	4.3188999791538615E-4	3.7231896372016045E-5	3.7231896372016046E-6	0.004702388511785626	3.7231896372016045E-5
38	4.5050594610139414E-4	4.095508600921765E-5	3.7231896372016046E-6	0.006091138246461825	3.7231896372016045E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	24955	0.0	31.271374	1
GTATCAA	68035	0.0	26.65713	1
TATCAAC	96090	0.0	18.700287	2
ATCAACG	97395	0.0	18.366789	3
TCAACGC	98100	0.0	18.160822	4
CAACGCA	98995	0.0	17.963062	5
AACGCAG	102590	0.0	17.60165	6
ACGCAGA	116440	0.0	15.4132595	7
CGCAGAG	116940	0.0	15.292714	8
AGAGTAC	122210	0.0	14.528549	11
TCTAGAC	5165	0.0	14.524794	3
GCAGAGT	122680	0.0	14.433547	9
CAGAGTA	123005	0.0	14.412972	10
GTATTAG	8525	0.0	14.224042	1
GAGTACT	71615	0.0	13.875445	12
AGTACTT	72680	0.0	13.732457	13
ACCGTCG	1410	0.0	12.949992	8
GTACTTT	77380	0.0	12.929823	14
GTACATG	96910	0.0	12.848728	1
CTAGACA	7435	0.0	12.694133	4
>>END_MODULE