FastQCFastQC Report
Wed 25 May 2016
SRR2558181_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2558181_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31685347
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1869570.5900424571648214No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1063000.3354863053890494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT915360.28889063452579516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA358200.035.818741
GTATCAA1032500.028.386791
TATCAAC1504700.019.3632582
ATCAACG1561050.018.6156653
TCAACGC1575500.018.4058574
CAACGCA1592550.018.2126315
AACGCAG1647550.017.8035726
TCTAGAC78150.016.1024683
ACGCAGA1877200.015.5633357
CGCAGAG1881250.015.4864888
ACCGTCG59050.015.4611328
TACCGTC61350.015.2401597
GGCGTTA69200.015.16445342
AGAGTAC1939700.014.97289911
CGTCGTA60100.014.97076910
ATACCGT64650.014.836656
CAGAGTA1956650.014.8126510
GCAGAGT1959250.014.8044079
CCGTCGT62150.014.724929
CTAGACA94600.014.4652314
Produced by FastQC (version 0.11.5)