FastQCFastQC Report
Wed 25 May 2016
SRR2558179_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2558179_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15162246
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1142300.7533844260276479No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT896490.5912646450928181No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC504090.33246393707106453No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG449220.296275367119093No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT439870.2901087345502771No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG432750.28541286033744606No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA387810.25577345203342566No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG383590.2529902232162702No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285420.18824387890817756No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC262980.1734439607430192No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT241880.15952781665724194No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237250.15647417935311167No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC220930.14571060250572376No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA213260.14065198520060945No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC212340.14004521493715377No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC170870.11269438577899343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCGA31750.020.789620
GTACTAG35450.020.6651151
TGCGACG30600.020.63964322
CGACGAG30200.020.62134224
CAATGCG31900.020.27820819
CGTCATT33850.019.5035826
GTCGTCA33450.019.40721924
CATTGCG33650.019.28785929
ATCGTCA33500.019.24249310
GCTCGTC33800.018.54711739
AGGTCGT35100.018.43240222
GACGAGT34750.017.79515825
GTCAGCG35800.017.63437343
TAGGTCG36900.017.3528621
TCAGCGC36800.017.21648644
TAGGCAT43050.016.9645295
CGTCATC39450.016.84096312
CGACGAA34200.016.78620738
TCGTCAG36950.016.7859441
TCGACGA33650.016.66810237
Produced by FastQC (version 0.11.5)