##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2558179_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15162246
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.379209386261113	31.0	30.0	33.0	26.0	34.0
2	30.489089611130172	31.0	30.0	34.0	26.0	34.0
3	30.732345458581797	31.0	30.0	34.0	27.0	34.0
4	34.355937306385876	35.0	35.0	37.0	30.0	37.0
5	33.9366589883847	35.0	35.0	37.0	28.0	37.0
6	33.99921515585488	35.0	35.0	37.0	29.0	37.0
7	33.84917590705229	35.0	35.0	37.0	28.0	37.0
8	33.83260936407442	35.0	35.0	37.0	28.0	37.0
9	35.17864596049952	38.0	35.0	39.0	28.0	39.0
10	35.0890895715582	38.0	35.0	39.0	27.0	39.0
11	35.19724168833562	38.0	35.0	39.0	27.0	39.0
12	35.06706420671449	38.0	35.0	39.0	27.0	39.0
13	35.074450183699696	38.0	35.0	39.0	27.0	39.0
14	35.90773009486853	39.0	35.0	40.0	27.0	41.0
15	35.884597572153886	38.0	35.0	40.0	27.0	41.0
16	35.8071582534672	38.0	34.0	40.0	27.0	41.0
17	35.82255425746291	38.0	34.0	40.0	27.0	41.0
18	35.75386469788183	38.0	34.0	40.0	27.0	41.0
19	35.84456319993753	39.0	34.0	40.0	27.0	41.0
20	35.81939509489557	39.0	34.0	40.0	27.0	41.0
21	35.72008995237249	39.0	34.0	40.0	27.0	41.0
22	35.69352185685419	39.0	34.0	40.0	27.0	41.0
23	35.626236970433006	39.0	34.0	40.0	27.0	41.0
24	35.593517279695895	39.0	34.0	40.0	26.0	41.0
25	35.50761760493795	38.0	34.0	40.0	26.0	41.0
26	35.36909307499694	38.0	34.0	40.0	25.0	41.0
27	35.17010936242559	38.0	34.0	40.0	25.0	41.0
28	35.13245544228737	38.0	34.0	40.0	25.0	41.0
29	35.04158447237962	38.0	33.0	40.0	25.0	41.0
30	34.92190952448602	38.0	33.0	40.0	24.0	41.0
31	34.82492996090421	38.0	33.0	40.0	24.0	41.0
32	34.7158683482645	38.0	33.0	40.0	24.0	41.0
33	34.606217574889634	38.0	33.0	40.0	24.0	41.0
34	34.54130548996501	38.0	33.0	40.0	24.0	41.0
35	34.45614257940413	38.0	33.0	40.0	24.0	41.0
36	34.364863424587625	38.0	33.0	40.0	23.0	41.0
37	34.271870077823564	38.0	32.0	40.0	23.0	41.0
38	34.18603939020644	38.0	32.0	40.0	23.0	41.0
39	34.10710319566112	38.0	32.0	40.0	22.0	41.0
40	33.95527351290831	38.0	32.0	40.0	22.0	41.0
41	33.829828245762535	37.0	31.0	40.0	21.0	41.0
42	33.743489585909636	37.0	31.0	40.0	21.0	41.0
43	33.60908047528051	37.0	31.0	40.0	20.0	41.0
44	33.44346213615054	37.0	31.0	40.0	19.0	41.0
45	33.29917685018433	37.0	30.0	40.0	19.0	41.0
46	33.106711103355	37.0	30.0	40.0	18.0	41.0
47	32.97608586485142	37.0	30.0	40.0	18.0	41.0
48	32.85268290726849	37.0	30.0	40.0	18.0	41.0
49	32.70900478728547	36.0	30.0	40.0	17.0	41.0
50	31.397985166577563	35.0	28.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	58.0
3	104.0
4	143.0
5	176.0
6	222.0
7	354.0
8	800.0
9	2135.0
10	5443.0
11	13517.0
12	28064.0
13	48067.0
14	68273.0
15	83308.0
16	94067.0
17	102080.0
18	107975.0
19	114785.0
20	121453.0
21	128190.0
22	137194.0
23	149616.0
24	166642.0
25	187652.0
26	212425.0
27	242145.0
28	278270.0
29	326170.0
30	389564.0
31	475566.0
32	588156.0
33	741025.0
34	946294.0
35	1212387.0
36	1556951.0
37	1992792.0
38	2339637.0
39	2300546.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.08140410844574	20.13306522006194	13.374524585634228	24.41100608585809
2	14.91565293495045	22.344042470968873	37.23037060259041	25.509933991490268
3	17.560193848638374	28.329901209849083	29.08426047274728	25.025644468765257
4	12.691625447070528	18.660568580451063	36.03171142021906	32.61609455225935
5	13.144560990722368	38.87704342028594	33.92791678742625	14.050478801565438
6	30.69469755266841	37.19077193344704	18.15280344592943	13.961727067955119
7	26.667571671322627	32.38979342081404	23.09269114414026	17.849943763723072
8	27.786873642519144	32.911708119402775	20.38183414059316	18.919584097484922
9	27.6125884370408	14.460031721630685	19.88014411151069	38.04723572981783
10	16.675227814824048	28.34427174753164	31.705409077869025	23.27509135977529
11	33.161254083215944	24.00899976281004	22.55152153798298	20.278224615991036
12	23.54871041224659	25.80991414181557	29.737623483184716	20.903751962753123
13	29.102322810725656	21.58728368161311	25.164290817406933	24.146102690254303
14	23.342738179031254	20.92121746779824	26.951325156986943	28.784719196183563
15	25.359982299229312	27.052130326542013	24.15733657759504	23.430550796633636
16	22.841851602298906	27.288324192928215	26.384738216822228	23.485085987950647
17	22.616063911187286	27.264326573327068	27.0657305132275	23.053879002258142
18	23.56064510629628	25.85707869094471	28.73605897921683	21.846217223542176
19	23.2537585183316	26.83178113041408	28.981556218481053	20.932904132773267
20	24.167003985475333	25.593745433000237	28.4283714643793	21.81087911714513
21	24.01291248604222	26.25126413478544	27.49442843687284	22.241394942299497
22	23.992657713363513	25.882339045005192	26.674867581423673	23.45013566020762
23	22.5170081761437	26.397921846775784	27.505582740750622	23.57948723632989
24	22.81132955403526	27.738053417797893	26.77202434382445	22.678592684342394
25	23.559108899497687	25.419265494255832	26.800237959000107	24.22138764724637
26	23.512616185209563	25.893153487407172	27.318613427817194	23.275616899566067
27	23.813528288130172	25.67581726946716	26.898938679634327	23.61171576276834
28	22.94849648505235	25.6833483982431	28.146794381204376	23.221360735500173
29	23.306705723631126	25.14864203544058	28.69604439396391	22.848607846964384
30	23.445904761314136	26.26436770613551	28.195943966284187	22.093783566266165
31	24.58222635757132	25.060763235866673	27.176053689149327	23.18095671741268
32	22.169881353120527	26.143007082018947	28.304630940674713	23.382480624185813
33	23.190834942211676	26.455013887750766	27.295945600184528	23.05820556985303
34	22.165433645688093	26.343839835634142	28.028401236226713	23.46232528245105
35	23.045332123122265	26.95003064006401	28.016245500462073	21.988391736351648
36	23.77821830351913	25.9438137942899	27.037848596116643	23.24011930607433
37	23.74297062668438	26.50431557021854	27.17027078010049	22.582443022996586
38	23.91050897022273	26.076319216165622	26.953639833147353	23.059531980464293
39	23.929149061374254	25.33151769789457	27.3662258302766	23.373107410454573
40	23.428974892796006	26.128688859353993	27.48867862629799	22.953657621552008
41	22.552889959940654	26.21419713177345	27.395352260925165	23.837560647360736
42	24.270995465167914	26.52872812300379	27.046063624852472	22.154212786975823
43	24.34073702375497	24.9589292126149	26.71452564328844	23.985808120341694
44	23.636453939928963	26.320026457385616	27.383110489398316	22.660409113287105
45	25.216317173854442	25.321617179599638	26.48034598874852	22.98171965779741
46	23.0345218548659	25.72331789707211	28.275574829388777	22.966585418673212
47	24.207800681598098	26.299519403884307	26.819335740326522	22.673344174191076
48	24.641586520000665	26.408793784649475	26.288992739626497	22.66062695572336
49	23.915222046104038	26.331111834579296	27.649988761033185	22.103677358283488
50	24.26462886266907	26.573424451589972	25.607359766633696	23.554586919107262
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2259.0
1	1954.0
2	1649.0
3	2961.5
4	4274.0
5	5316.0
6	6358.0
7	5698.5
8	5039.0
9	7132.0
10	9225.0
11	14620.0
12	20015.0
13	40592.0
14	61169.0
15	79987.5
16	98806.0
17	100852.5
18	102899.0
19	99376.0
20	95853.0
21	103683.5
22	111514.0
23	127420.5
24	143327.0
25	173435.0
26	203543.0
27	229097.0
28	254651.0
29	295858.0
30	337065.0
31	378286.5
32	419508.0
33	456103.0
34	492698.0
35	538178.5
36	583659.0
37	618786.5
38	653914.0
39	697501.5
40	741089.0
41	777167.0
42	813245.0
43	847030.5
44	880816.0
45	970550.0
46	1060284.0
47	1168864.5
48	1277445.0
49	1308692.5
50	1339940.0
51	1188860.5
52	1037781.0
53	929639.0
54	821497.0
55	779590.0
56	737683.0
57	704629.5
58	671576.0
59	631575.0
60	591574.0
61	517983.0
62	444392.0
63	390149.0
64	335906.0
65	289074.0
66	242242.0
67	207457.0
68	172672.0
69	151466.0
70	130260.0
71	108284.0
72	86308.0
73	74645.5
74	62983.0
75	53767.5
76	44552.0
77	34502.0
78	24452.0
79	18948.0
80	13444.0
81	11153.0
82	8862.0
83	7474.0
84	6086.0
85	5029.5
86	3973.0
87	3196.5
88	2420.0
89	1831.0
90	1242.0
91	1021.5
92	801.0
93	658.0
94	515.0
95	444.0
96	373.0
97	301.5
98	230.0
99	204.0
100	178.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.036063258701909996
2	0.00672064020066684
3	7.057001977147713E-4
4	0.005065212634064901
5	0.00836287710936757
6	0.02302429336656324
7	0.013869976783123029
8	0.018018438693053786
9	0.018954975404039745
10	0.017642504942869282
11	0.009246651188748685
12	0.01174628086102811
13	0.02279345685329205
14	0.029395381132847995
15	0.023637658958969535
16	0.051041250748734716
17	0.021995422050268807
18	0.034150613306234444
19	0.051905238841264016
20	0.039314755874558426
21	0.050203643972007835
22	0.05170078364379525
23	0.0376725189658577
24	0.04417551331115456
25	0.044149131995352135
26	0.04846247712904803
27	0.043502789758192814
28	0.05160185370953617
29	0.014292077835961769
30	0.031189310607412646
31	0.05504461542175216
32	0.04964963634015699
33	0.03775166291326496
34	0.01266303158516225
35	0.01553859500762618
36	0.017029139350462984
37	0.005711554871224224
38	0.0071493365824561875
39	0.004036341317770468
40	0.023274915866686244
41	0.00817820789875062
42	0.014892252770466855
43	0.0040759132914741
44	0.008323305135663938
45	0.01156161165041116
46	0.016402583100155476
47	0.01120546388707847
48	0.035944542780799095
49	0.0046826835549297905
50	0.012359646453434407
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1.5162246E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.89957614428105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.84563452983069	27.248726142562376
2	9.759998905701222	7.20279645578044
3	5.119298262671454	5.666998081461929
4	3.073327601210995	4.536179433488229
5	2.0135362566434853	3.7149317210643455
6	1.380378084414401	3.0561219740247605
7	0.9395792849002103	2.4269054156735113
8	0.6943654194520453	2.0497431733621116
9	0.525231409253219	1.7442734741196477
>10	2.399205738932357	14.905781650688372
>50	0.12813218153185324	3.296734855049735
>100	0.09573336919753435	7.275160744364315
>500	0.014920210097420946	3.8099740954811905
>1k	0.009433642112537376	6.098900503314621
>5k	7.175609440245123E-4	1.8137802081841723
>10k+	5.075431067490453E-4	5.1529920713802015
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	114230	0.7533844260276479	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	89649	0.5912646450928181	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	50409	0.33246393707106453	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	44922	0.296275367119093	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43987	0.2901087345502771	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	43275	0.28541286033744606	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	38781	0.25577345203342566	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	38359	0.2529902232162702	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28542	0.18824387890817756	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	26298	0.1734439607430192	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	24188	0.15952781665724194	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23725	0.15647417935311167	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	22093	0.14571060250572376	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	21326	0.14065198520060945	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	21234	0.14004521493715377	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	17087	0.11269438577899343	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.573927635786941E-5	0.0	0.0	0.0	2.6381315802421357E-5
2	2.8359914487602957E-4	0.0	0.0	1.3190657901210679E-5	2.6381315802421357E-5
3	4.352917107399524E-4	0.0	0.0	2.6381315802421357E-5	3.2976644753026695E-5
4	4.418870396905577E-4	0.0	0.0	5.935796055544805E-5	3.2976644753026695E-5
5	6.463422371593233E-4	0.0	0.0	6.595328950605339E-5	3.2976644753026695E-5
6	6.661282240111393E-4	0.0	0.0	6.595328950605339E-5	5.2762631604842714E-5
7	8.046301319738514E-4	0.0	0.0	7.254861845665873E-5	5.2762631604842714E-5
8	0.0012135405269113823	0.0	0.0	7.914394740726407E-5	7.254861845665873E-5
9	0.001226731184812593	0.0	0.0	9.892993425908008E-5	7.254861845665873E-5
10	0.010836125465844572	0.0	0.0	3.6274309228329364E-4	9.233460530847474E-5
11	0.010902078755350625	0.0	0.0	3.6274309228329364E-4	9.233460530847474E-5
12	0.012029880005904138	0.0	0.0	4.155057238881364E-4	9.233460530847474E-5
13	0.012076047308558376	0.0	0.0	4.221010528387417E-4	9.233460530847474E-5
14	0.012095833295410191	0.0	0.0	4.352917107399524E-4	9.233460530847474E-5
15	0.012260716519175326	0.0	0.0	4.418870396905577E-4	1.2531125006150145E-4
16	0.012287097834977747	0.0	0.0	4.6826835549297906E-4	2.2424118432058153E-4
17	0.014140385270097847	0.0	0.0	6.133655924062965E-4	2.2424118432058153E-4
18	0.014331649809665403	0.0	0.0	6.793188819123499E-4	2.4402717117239756E-4
19	0.014483342375529324	0.0	0.0	7.057001977147713E-4	2.4402717117239756E-4
20	0.014635034941393248	0.0	0.0	7.650581582702193E-4	2.704084869748189E-4
21	0.015202233231145307	0.0	0.0	9.101553951835368E-4	3.4295710543147764E-4
22	0.016917018758302694	0.0	0.0	0.0014311863822813586	3.4295710543147764E-4
23	0.017906318100893496	6.595328950605339E-6	0.0	0.0022819838169094472	3.4295710543147764E-4
24	0.018836259482928847	6.595328950605339E-6	0.0	0.002862372764562717	3.4295710543147764E-4
25	0.01884945014083006	6.595328950605339E-6	0.0	0.0029481120409205865	3.4295710543147764E-4
26	0.018935189417187928	6.595328950605339E-6	0.0	0.0031987345410435897	3.4295710543147764E-4
27	0.01894838007508914	6.595328950605339E-6	0.0	0.0034691430280184084	3.561477633326883E-4
28	0.019047310009348218	6.595328950605339E-6	0.0	0.004128675923078942	3.6274309228329364E-4
29	0.019192407246261537	6.595328950605339E-6	0.0	0.005190523884126402	3.6274309228329364E-4
30	0.019324313825273644	6.595328950605339E-6	0.0	0.006958072042888633	3.6274309228329364E-4
31	0.019878321457124492	6.595328950605339E-6	0.0	0.009022410004428104	3.6274309228329364E-4
32	0.019931084088729335	6.595328950605339E-6	0.0	0.011126319939671206	3.6274309228329364E-4
33	0.020168515930951127	6.595328950605339E-6	0.0	0.01381061882256758	3.8252907913510965E-4
34	0.02053125902323442	6.595328950605339E-6	0.0	0.01673894487663635	3.957197370363203E-4
35	0.02072911889175258	6.595328950605339E-6	0.0	0.02076209553650561	3.957197370363203E-4
36	0.02076209553650561	6.595328950605339E-6	0.0	0.02475226955162184	3.957197370363203E-4
37	0.02081485816811045	6.595328950605339E-6	0.0	0.031169524620560832	3.957197370363203E-4
38	0.02082804882601166	6.595328950605339E-6	0.0	0.03814738265030128	4.352917107399524E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATGCGA	3175	0.0	20.7896	20
GTACTAG	3545	0.0	20.665115	1
TGCGACG	3060	0.0	20.639643	22
CGACGAG	3020	0.0	20.621342	24
CAATGCG	3190	0.0	20.278208	19
CGTCATT	3385	0.0	19.50358	26
GTCGTCA	3345	0.0	19.407219	24
CATTGCG	3365	0.0	19.287859	29
ATCGTCA	3350	0.0	19.242493	10
GCTCGTC	3380	0.0	18.547117	39
AGGTCGT	3510	0.0	18.432402	22
GACGAGT	3475	0.0	17.795158	25
GTCAGCG	3580	0.0	17.634373	43
TAGGTCG	3690	0.0	17.35286	21
TCAGCGC	3680	0.0	17.216486	44
TAGGCAT	4305	0.0	16.964529	5
CGTCATC	3945	0.0	16.840963	12
CGACGAA	3420	0.0	16.786207	38
TCGTCAG	3695	0.0	16.78594	41
TCGACGA	3365	0.0	16.668102	37
>>END_MODULE