FastQCFastQC Report
Wed 25 May 2016
SRR2558178_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2558178_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10612128
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA488540.46036007104324417No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450570.424580253837873No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT332490.31331133586025345No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301130.2837602411128098No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261870.24676483359416695No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG219820.20714035865379687No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG198230.18679571147276022No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG188290.17742906983406156No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG184490.17384826115930754No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC180170.1697774470869556No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGAAAATTTA156000.14700161927937544No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGCTGAAGGG147990.1394536515202229No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAGGGCACAT147150.1386621043394878No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTA145650.13724862723103226No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACATGCTGAT137500.12956873494175722No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATC136810.12891853547186766No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG129500.1220301903633277No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC126800.11948593156810773No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA125330.11810072400182131No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAAGTTCTCT124270.1171018668451794No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT124260.11709244366445637No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA119390.11250335465233739No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA118940.11207931151980075No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT116930.11018525219447034No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGAAATCCAG108510.10225093402567327No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTT107740.10152534910999943No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGATGTTTGA107510.10130861595336958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCA29950.024.82876210
CAATGCG32150.023.81709319
CGACGAG32150.023.13542224
GTACTAG35600.022.5558721
AGTCCTA33100.022.00187929
TGCGACG34550.021.71971722
AATGCGA35550.021.66322120
GACGAGT35150.021.09824225
TAGGCAT40150.021.092825
CGTCATC36600.020.73771712
CAATTCG35550.020.30143715
AATTCGT35100.019.80982816
CCAATGC38550.019.7483118
GTCATCC40350.019.03138213
CTAGGCA46350.018.9831244
GCTCGTC28500.018.90756639
ATTCGTC36550.018.90255217
GTTAATA44050.018.8283481
TCGTCTC37350.018.73384919
GTCCTAC39500.018.5484230
Produced by FastQC (version 0.11.5)