##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2558178_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10612128
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.277150822153672	31.0	28.0	31.0	25.0	34.0
2	29.323496757671975	31.0	28.0	33.0	25.0	34.0
3	29.565202662463175	31.0	28.0	33.0	25.0	34.0
4	33.15602045131759	35.0	32.0	37.0	27.0	37.0
5	32.572974524996305	35.0	32.0	37.0	25.0	37.0
6	32.70365519526338	35.0	32.0	37.0	25.0	37.0
7	32.50458720437597	35.0	32.0	37.0	24.0	37.0
8	32.51114771702716	35.0	32.0	37.0	24.0	37.0
9	33.62290890196575	37.0	32.0	39.0	23.0	39.0
10	33.45757929041188	37.0	32.0	39.0	23.0	39.0
11	33.6220284941908	37.0	32.0	39.0	23.0	39.0
12	33.42289114869327	37.0	32.0	39.0	23.0	39.0
13	33.4483028286127	37.0	32.0	39.0	23.0	39.0
14	34.04485019404214	37.0	32.0	40.0	22.0	41.0
15	34.03741483329262	37.0	32.0	40.0	22.0	41.0
16	33.921002177885526	37.0	32.0	40.0	21.0	41.0
17	33.9581908548408	37.0	32.0	40.0	22.0	41.0
18	33.89146314480941	37.0	32.0	40.0	21.0	41.0
19	34.00141573867183	37.0	32.0	40.0	21.0	41.0
20	33.971090623859794	37.0	32.0	40.0	20.0	41.0
21	33.82894769079302	37.0	31.0	40.0	19.0	41.0
22	33.79263763120837	37.0	31.0	40.0	19.0	41.0
23	33.69201285548007	37.0	31.0	40.0	19.0	41.0
24	33.63638508694957	37.0	31.0	40.0	19.0	41.0
25	33.52870715468189	37.0	31.0	40.0	18.0	41.0
26	33.36751120981579	37.0	31.0	40.0	18.0	41.0
27	33.14500776847019	37.0	30.0	40.0	18.0	41.0
28	33.08600697239988	37.0	30.0	40.0	18.0	41.0
29	33.008536459417	37.0	30.0	39.0	18.0	41.0
30	32.86234419712993	36.0	30.0	39.0	17.0	41.0
31	32.74831296795516	36.0	30.0	39.0	17.0	41.0
32	32.56778847748538	36.0	30.0	39.0	17.0	41.0
33	32.445955231599164	36.0	30.0	39.0	16.0	40.0
34	32.31822580730274	36.0	30.0	39.0	16.0	40.0
35	32.21866632215518	35.0	29.0	39.0	16.0	40.0
36	32.10795261798576	35.0	29.0	39.0	16.0	40.0
37	31.985533815649415	35.0	29.0	39.0	16.0	40.0
38	31.876640481532075	35.0	28.0	39.0	15.0	40.0
39	31.77992481809492	35.0	28.0	39.0	15.0	40.0
40	31.58533773810493	35.0	28.0	39.0	15.0	40.0
41	31.445008484631924	35.0	27.0	39.0	15.0	40.0
42	31.366279317399865	35.0	27.0	39.0	14.0	40.0
43	31.169404006434902	35.0	27.0	39.0	13.0	40.0
44	30.98866447898103	35.0	26.0	39.0	13.0	40.0
45	30.819708073630473	35.0	26.0	39.0	12.0	40.0
46	30.548037773385317	34.0	26.0	38.0	12.0	40.0
47	30.37606934254845	34.0	25.0	38.0	10.0	40.0
48	30.232470339596357	34.0	25.0	38.0	10.0	40.0
49	30.054797020917952	34.0	24.0	38.0	10.0	40.0
50	28.860552473547248	33.0	23.0	38.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
1	3.0
2	33.0
3	74.0
4	81.0
5	126.0
6	205.0
7	297.0
8	514.0
9	1485.0
10	4659.0
11	14699.0
12	35449.0
13	62236.0
14	85167.0
15	99583.0
16	108187.0
17	112640.0
18	118290.0
19	124757.0
20	131247.0
21	140936.0
22	152299.0
23	166467.0
24	184450.0
25	205827.0
26	232167.0
27	264557.0
28	303399.0
29	351841.0
30	411261.0
31	485050.0
32	571378.0
33	668944.0
34	776194.0
35	880267.0
36	965504.0
37	995574.0
38	1023825.0
39	932456.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.10961343082195	24.082226474974817	14.046485505941714	21.76167458826151
2	14.965590980771548	25.41911755420695	37.12713667541758	22.48815478960392
3	17.359759074495972	31.190340967182955	30.463829088311368	20.98607087000971
4	12.846337613755903	20.86989889505371	35.736469560285975	30.547293930904413
5	12.193179488991767	41.56862800532448	33.20011779811758	13.038074707566171
6	28.30613884514505	39.285098948627244	18.497178255317657	13.91158395091005
7	24.25084708895723	34.96317084436342	23.26327325935742	17.522708807321933
8	27.31969708293745	33.680442686948346	20.210734911567506	18.78912531854669
9	27.137161976658238	15.00642161999749	20.419384989101577	37.437031414242696
10	16.521997615715055	30.03406404092473	31.654309030962246	21.78962931239797
11	31.991292639992658	25.311384056152463	22.969087628011508	19.728235675843372
12	22.463407674021823	28.07317862046139	29.439227410731988	20.024186294784794
13	28.010724108225084	23.118059443492488	26.822108741590274	22.049107706692155
14	23.016528867194552	22.42348606350354	28.0699669102174	26.490018159084517
15	24.74718817833165	28.548116953684705	25.549069857969194	21.155625010014447
16	22.088135999746573	29.36048923463562	26.184685999520298	22.36668876609751
17	21.39039882690595	28.728117942022212	27.31693473571029	22.564548495361546
18	21.102890031961692	27.32466951220972	30.86492638026921	20.707514075559377
19	22.287794419627282	26.947671513398436	32.11631401994634	18.648220047027944
20	23.976699809633914	25.640390415443765	31.010902921969514	19.372006852952804
21	22.789130621906402	27.19651536388628	28.95209850973749	21.062255504469825
22	22.701221409901635	27.0129966370663	28.87414553680366	21.411636416228404
23	21.473110688521462	27.218700135565744	29.109588234013188	22.198600941899603
24	22.64122839202868	28.107888170440564	28.67160634981449	20.579277087716267
25	22.98550611782804	26.09808529691563	29.076334244988967	21.840074340267364
26	22.555606267004926	26.328544385408186	28.597106040783256	22.51874330680363
27	22.51288353043379	26.10651767823533	29.57518405548509	21.805414735845794
28	20.77121473136706	27.205732057950378	29.995186012623094	22.027867198059468
29	22.12316829077919	26.698645652443126	30.35377059309276	20.82441546368493
30	21.627682350538073	28.027213910966132	29.472716100225483	20.872387638270315
31	23.582565023449767	26.01173699868069	29.1851843750439	21.220513602825644
32	21.096810238552358	27.68370929926919	30.0280800677904	21.19140039438806
33	22.67796257704146	27.02447670348103	28.893220804090312	21.404339915387194
34	20.872330894908806	27.664769890865102	29.730332633324135	21.732566580901956
35	22.142107149558683	27.986919836830648	29.03343279075179	20.837540222858884
36	22.46532510418538	27.27065126770737	28.22106658609174	22.042957042015516
37	23.227909259455064	27.013995415213778	28.60739050350286	21.1507048218283
38	22.919410952818666	27.301690005938873	28.637210663734486	21.14168837750798
39	23.129383117189075	26.19233980478225	29.51462248415434	21.16365459387433
40	22.295851014517122	26.661270964114088	29.226428926495224	21.816449094873565
41	21.687716633085984	27.366542710290766	29.241805402939896	21.70393525368335
42	23.133109011522293	26.922971441237948	28.50526862211727	21.438650925122488
43	22.777784217200665	26.19846533789018	28.55960067536667	22.46414976954248
44	22.606715973861068	26.59246776407561	29.22337116533717	21.57744509672615
45	24.448778737202883	26.11411910525923	27.859810174061145	21.577291983476744
46	22.61924800550401	27.02507433709221	29.271944168249224	21.08373348915456
47	23.163560678562863	27.34505989328953	27.366810998633017	22.12456842951459
48	24.373626663675296	26.95206296207966	27.865405263242238	20.808905111002808
49	23.620534280137424	27.062443064018304	29.209460238176753	20.10756241766752
50	24.276070781928375	27.32815598834551	26.51888011924405	21.87689311048207
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2879.0
1	2350.5
2	1822.0
3	2728.0
4	3634.0
5	4890.0
6	6146.0
7	5796.0
8	5446.0
9	7515.0
10	9584.0
11	15058.0
12	20532.0
13	42659.5
14	64787.0
15	89752.0
16	114717.0
17	117729.5
18	120742.0
19	119767.0
20	118792.0
21	134117.5
22	149443.0
23	160245.0
24	171047.0
25	185497.0
26	199947.0
27	198262.0
28	196577.0
29	222274.0
30	247971.0
31	317462.5
32	386954.0
33	401210.0
34	415466.0
35	439921.0
36	464376.0
37	466089.5
38	467803.0
39	511786.0
40	555769.0
41	603145.5
42	650522.0
43	656089.5
44	661657.0
45	703921.5
46	746186.0
47	776165.5
48	806145.0
49	831741.5
50	857338.0
51	837595.5
52	817853.0
53	685038.0
54	552223.0
55	496924.5
56	441626.0
57	407103.0
58	372580.0
59	344590.5
60	316601.0
61	268211.5
62	219822.0
63	187499.0
64	155176.0
65	127740.5
66	100305.0
67	82273.5
68	64242.0
69	53679.5
70	43117.0
71	35542.0
72	27967.0
73	23672.5
74	19378.0
75	16354.0
76	13330.0
77	10544.5
78	7759.0
79	6301.0
80	4843.0
81	4112.5
82	3382.0
83	2823.0
84	2264.0
85	1862.0
86	1460.0
87	1187.5
88	915.0
89	670.5
90	426.0
91	326.5
92	227.0
93	199.0
94	171.0
95	130.0
96	89.0
97	68.5
98	48.0
99	45.0
100	42.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03367844790413384
2	0.006275838361542568
3	4.8058221687488124E-4
4	0.004532549927780743
5	0.007095655084446777
6	0.022427170120827792
7	0.012975719855621794
8	0.01725384390388054
9	0.017687310217140238
10	0.017140765735204098
11	0.008245283132657277
12	0.010930889638722789
13	0.022502555566612088
14	0.029334361590813828
15	0.02316217821722467
16	0.05201595759116362
17	0.021079655277433516
18	0.032896323904121776
19	0.05341058833817308
20	0.03906850727771093
21	0.05031036188029394
22	0.05291115975985212
23	0.03760791426564022
24	0.04428894939827337
25	0.044166448048873895
26	0.04970727831401958
27	0.043280669060908425
28	0.052166728482732204
29	0.013286684819482012
30	0.030992841398068325
31	0.05502195224181239
32	0.050093628723664095
33	0.03807907330179206
34	0.012391482650793506
35	0.014671892385768433
36	0.016151331759285226
37	0.004805822168748813
38	0.006464301976003305
39	0.0036561941205383126
40	0.023086792771440375
41	0.008179320867596018
42	0.013936884289371556
43	0.003872927277168161
44	0.007821240000120617
45	0.0107801187471542
46	0.015812097253255896
47	0.01105339098812227
48	0.03616616761501558
49	0.004042544530182825
50	0.012099364048379363
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1.0612128E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.47064562501228
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.77059883860957	31.01467777251592
2	7.158660018025118	5.3647922537072645
3	3.3062022107917386	3.7165659421562824
4	1.8520023662018406	2.7758289744253357
5	1.1933459148995067	2.235772094262774
6	0.7936866055707866	1.7843969720797108
7	0.5465845511072251	1.4336613213051674
8	0.395583939127959	1.1858228478408144
9	0.28881125928883794	0.9739749914393026
>10	1.4537124862638076	9.641575237686284
>50	0.10141409952485785	2.648469299633816
>100	0.0989191864736533	8.029039855786946
>500	0.01888481511885925	5.0098342217301
>1k	0.019028845447657372	14.614127211165759
>5k	0.0017831908480496668	4.533765163181152
>10k+	7.816727005149225E-4	5.037695841083676
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	48854	0.46036007104324417	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45057	0.424580253837873	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	33249	0.31331133586025345	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30113	0.2837602411128098	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26187	0.24676483359416695	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG	21982	0.20714035865379687	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	19823	0.18679571147276022	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	18829	0.17742906983406156	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	18449	0.17384826115930754	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	18017	0.1697774470869556	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGAAAATTTA	15600	0.14700161927937544	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGCTGAAGGG	14799	0.1394536515202229	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAGGGCACAT	14715	0.1386621043394878	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTA	14565	0.13724862723103226	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACATGCTGAT	13750	0.12956873494175722	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATC	13681	0.12891853547186766	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	12950	0.1220301903633277	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	12680	0.11948593156810773	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	12533	0.11810072400182131	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAAGTTCTCT	12427	0.1171018668451794	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	12426	0.11709244366445637	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	11939	0.11250335465233739	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	11894	0.11207931151980075	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	11693	0.11018525219447034	No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGAAATCCAG	10851	0.10225093402567327	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTT	10774	0.10152534910999943	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGATGTTTGA	10751	0.10130861595336958	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3192453012251642E-4	0.0	0.0	0.0	0.0
2	3.39234506029328E-4	0.0	0.0	1.8846361446073774E-5	0.0
3	4.617358554288075E-4	0.0	0.0	2.8269542169110662E-5	0.0
4	4.617358554288075E-4	0.0	0.0	4.711590361518444E-5	0.0
5	4.99428578320955E-4	0.0	0.0	5.6539084338221325E-5	0.0
6	5.182749397670288E-4	0.0	9.423180723036887E-6	5.6539084338221325E-5	0.0
7	6.219299277204346E-4	0.0	9.423180723036887E-6	7.53854457842951E-5	0.0
8	9.894339759188731E-4	0.0	9.423180723036887E-6	1.7904043373770086E-4	0.0
9	9.9885715664191E-4	0.0	9.423180723036887E-6	2.450026987989591E-4	0.0
10	0.009149908482068817	0.0	9.423180723036887E-6	0.0035336927711388326	0.0
11	0.009197024385684002	0.0	9.423180723036887E-6	0.0035619623133079435	0.0
12	0.009950878843526953	0.0	9.423180723036887E-6	0.003910620000060309	9.423180723036887E-6
13	0.00996030202424999	0.0	9.423180723036887E-6	0.0039200431807833456	9.423180723036887E-6
14	0.0099885715664191	0.0	9.423180723036887E-6	0.003976582265121566	9.423180723036887E-6
15	0.01005453383148036	0.0	9.423180723036887E-6	0.00399542862656764	9.423180723036887E-6
16	0.010073380192926433	0.0	9.423180723036887E-6	0.004004851807290677	9.423180723036887E-6
17	0.011515126843551077	0.0	9.423180723036887E-6	0.004777552626579702	9.423180723036887E-6
18	0.011637628192950555	0.0	9.423180723036887E-6	0.004890630795256145	9.423180723036887E-6
19	0.011797822265242184	0.0	9.423180723036887E-6	0.004984862602486513	9.423180723036887E-6
20	0.01195801633753381	0.0	9.423180723036887E-6	0.005163903036224215	9.423180723036887E-6
21	0.01259879262670032	0.0	9.423180723036887E-6	0.005531407084422653	9.423180723036887E-6
22	0.014285541976123922	0.0	9.423180723036887E-6	0.007133347807338924	9.423180723036887E-6
23	0.015510555470118717	9.423180723036887E-6	9.423180723036887E-6	0.009498566168821183	9.423180723036887E-6
24	0.016490566265314553	9.423180723036887E-6	9.423180723036887E-6	0.011175892337521748	9.423180723036887E-6
25	0.016509412626760627	9.423180723036887E-6	9.423180723036887E-6	0.011279547325475155	9.423180723036887E-6
26	0.016575374891821884	9.423180723036887E-6	9.423180723036887E-6	0.011486857301381966	9.423180723036887E-6
27	0.016575374891821884	9.423180723036887E-6	9.423180723036887E-6	0.01165647455439663	9.423180723036887E-6
28	0.016584798072544923	9.423180723036887E-6	9.423180723036887E-6	0.012400905831516543	9.423180723036887E-6
29	0.016603644433990997	2.8269542169110662E-5	9.423180723036887E-6	0.01353168751828097	9.423180723036887E-6
30	0.016603644433990997	2.8269542169110662E-5	9.423180723036887E-6	0.01570844226530249	9.423180723036887E-6
31	0.016782684867728697	2.8269542169110662E-5	9.423180723036887E-6	0.01774384930147846	9.423180723036887E-6
32	0.01682980077134388	2.8269542169110662E-5	9.423180723036887E-6	0.01992060404849998	9.423180723036887E-6
33	0.016961725301466397	2.8269542169110662E-5	9.423180723036887E-6	0.02261563373528853	9.423180723036887E-6
34	0.017131342554481063	2.8269542169110662E-5	9.423180723036887E-6	0.025687590650998557	9.423180723036887E-6
35	0.01715961209665017	2.8269542169110662E-5	9.423180723036887E-6	0.029532248385997606	9.423180723036887E-6
36	0.017216151180988393	2.8269542169110662E-5	9.423180723036887E-6	0.034140183759562646	9.423180723036887E-6
37	0.017234997542434467	2.8269542169110662E-5	9.423180723036887E-6	0.04116987657894816	9.423180723036887E-6
38	0.01725384390388054	4.711590361518444E-5	9.423180723036887E-6	0.0485105343621939	9.423180723036887E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTCA	2995	0.0	24.828762	10
CAATGCG	3215	0.0	23.817093	19
CGACGAG	3215	0.0	23.135422	24
GTACTAG	3560	0.0	22.555872	1
AGTCCTA	3310	0.0	22.001879	29
TGCGACG	3455	0.0	21.719717	22
AATGCGA	3555	0.0	21.663221	20
GACGAGT	3515	0.0	21.098242	25
TAGGCAT	4015	0.0	21.09282	5
CGTCATC	3660	0.0	20.737717	12
CAATTCG	3555	0.0	20.301437	15
AATTCGT	3510	0.0	19.809828	16
CCAATGC	3855	0.0	19.74831	18
GTCATCC	4035	0.0	19.031382	13
CTAGGCA	4635	0.0	18.983124	4
GCTCGTC	2850	0.0	18.907566	39
ATTCGTC	3655	0.0	18.902552	17
GTTAATA	4405	0.0	18.828348	1
TCGTCTC	3735	0.0	18.733849	19
GTCCTAC	3950	0.0	18.54842	30
>>END_MODULE