FastQCFastQC Report
Wed 25 May 2016
SRR2558177_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2558177_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8462398
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA549450.6492840445462386No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271000.3202401966912925No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC229960.2717433049119174No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT229050.27066795960199463No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG216040.25529406676452704No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206460.24397339855676842No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG200060.2364105304430257No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG193650.2288358453478553No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180620.21343831854753226No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC136450.1612427115812799No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT129810.15339623591327187No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG119730.14148471863412712No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA102830.1215140200212753No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT100450.11870157844147723No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA99120.11712991991159007No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC97130.1147783406074732No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA90810.10731000834515228No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA89360.10559654603813245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTCG27000.023.79574815
AATTCGT26600.023.07811416
GCATATA8200.022.536911
CAATGCG28550.021.8088319
AATGCGA30000.021.41503320
TCGTCTC28050.021.41321219
ATCGTCA30300.021.34696810
ATTCGTC29250.021.2869117
GTACTAG30450.021.0971831
TAGGCAT32300.021.043885
GTGATCG32850.020.9591528
GTTAATA32300.020.6380671
TGCGACG30550.020.38469722
TGATCGC34150.020.3563379
GGCAATT32550.020.34654413
CGACGAG31750.019.89160324
AGTCCTA31400.019.33929
GGTATAG24700.019.3282151
CGTCTCC32600.019.30214720
TTAATAA33950.019.2419052
Produced by FastQC (version 0.11.5)