##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2558177_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8462398
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.255145645477796	30.0	27.0	31.0	22.0	34.0
2	28.216861225387888	31.0	27.0	31.0	20.0	34.0
3	28.42831287301779	31.0	27.0	31.0	22.0	34.0
4	31.826487125753243	35.0	30.0	37.0	23.0	37.0
5	31.081110696991562	35.0	30.0	35.0	18.0	37.0
6	31.324901405015456	35.0	30.0	36.0	19.0	37.0
7	31.071699416642893	35.0	30.0	36.0	17.0	37.0
8	31.08340650014334	35.0	30.0	37.0	17.0	37.0
9	32.019736013361694	35.0	30.0	39.0	17.0	39.0
10	31.733027564999897	35.0	29.0	39.0	17.0	39.0
11	31.996111267751765	35.0	30.0	39.0	17.0	39.0
12	31.7154815928062	35.0	29.0	39.0	17.0	39.0
13	31.80671826118318	35.0	29.0	39.0	17.0	39.0
14	32.202294668721564	36.0	29.0	39.0	17.0	40.0
15	32.145528253339066	36.0	28.0	39.0	17.0	40.0
16	32.01680587464688	36.0	28.0	39.0	17.0	40.0
17	32.02054843083485	36.0	28.0	39.0	17.0	40.0
18	31.971061866860907	36.0	28.0	39.0	16.0	40.0
19	31.986347605016924	36.0	28.0	39.0	16.0	40.0
20	31.965906472373433	36.0	28.0	39.0	16.0	40.0
21	31.818623515462164	36.0	27.0	39.0	16.0	40.0
22	31.74192657920367	36.0	27.0	39.0	16.0	40.0
23	31.6000830970134	35.0	27.0	39.0	15.0	40.0
24	31.490938029622335	35.0	27.0	39.0	15.0	40.0
25	31.401164658055553	35.0	27.0	39.0	14.0	40.0
26	31.201002599972252	35.0	27.0	39.0	13.0	40.0
27	30.937235166674977	34.0	27.0	39.0	13.0	40.0
28	30.825621295524034	34.0	26.0	39.0	13.0	40.0
29	30.68696189897946	34.0	26.0	39.0	13.0	40.0
30	30.528277327537655	34.0	25.0	38.0	12.0	40.0
31	30.384424367655598	34.0	25.0	38.0	12.0	40.0
32	30.158541704136347	34.0	25.0	38.0	12.0	40.0
33	29.986058088971944	33.0	25.0	38.0	11.0	40.0
34	29.850060703833595	33.0	25.0	38.0	10.0	40.0
35	29.745861634019104	33.0	25.0	38.0	9.0	40.0
36	29.612836692389084	33.0	24.0	38.0	9.0	40.0
37	29.463121564360364	33.0	24.0	38.0	9.0	40.0
38	29.31017283753376	33.0	24.0	38.0	9.0	40.0
39	29.20225850875839	33.0	24.0	38.0	9.0	40.0
40	28.956224110470814	33.0	23.0	38.0	9.0	40.0
41	28.74905446423106	32.0	23.0	38.0	9.0	40.0
42	28.634773618541693	32.0	22.0	38.0	9.0	40.0
43	28.44534870612325	32.0	22.0	38.0	9.0	40.0
44	28.215332226160953	32.0	22.0	37.0	8.0	40.0
45	28.047522463490846	31.0	21.0	37.0	8.0	40.0
46	27.730552261900232	31.0	20.0	37.0	8.0	40.0
47	27.526412253358917	31.0	20.0	37.0	8.0	40.0
48	27.375243636614584	31.0	20.0	37.0	8.0	40.0
49	27.15390046651079	30.0	19.0	37.0	8.0	40.0
50	25.92489256591335	29.0	15.0	35.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	25.0
3	82.0
4	88.0
5	107.0
6	155.0
7	278.0
8	649.0
9	2295.0
10	8523.0
11	25889.0
12	55668.0
13	89520.0
14	113272.0
15	128553.0
16	136783.0
17	143313.0
18	150876.0
19	159006.0
20	167132.0
21	177756.0
22	190204.0
23	205292.0
24	222674.0
25	241051.0
26	265211.0
27	289761.0
28	316247.0
29	345752.0
30	378626.0
31	413878.0
32	451373.0
33	491585.0
34	531794.0
35	566792.0
36	594007.0
37	612934.0
38	632981.0
39	352266.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.122122747108136	25.695092193725916	13.07327116595107	21.109513893214878
2	17.517163546335222	26.21735469556457	32.9559352278871	23.3095465302131
3	19.349718122401065	30.54656968663946	27.505680743234663	22.598031447724814
4	14.687037309057727	22.032021330063518	32.26402547752335	31.016915883355413
5	14.14301109489579	40.557934411029116	31.563331713902176	13.735722780172916
6	31.42157265415898	37.539413273070146	16.657792265737978	14.3812218070329
7	28.051922910782285	32.378792747650394	21.566605561099088	18.00267878046823
8	30.322555101119423	31.540609393531643	18.686472754219785	19.45036275112915
9	29.11726071991742	12.861418934299456	19.305661039216755	38.715659306566366
10	18.956025058840243	28.69644013281573	28.520019865156154	23.827514943187875
11	35.31915828987613	23.781316279696938	20.346433306589528	20.553092123837406
12	24.84381379974201	26.36356169418433	26.958252846557983	21.83437165951568
13	31.25748167521445	20.84485724065417	23.829159196810846	24.068501887320533
14	25.61115807629352	20.14894976080853	25.85468751920766	28.385204643690294
15	27.06009460510271	26.666259290221795	22.699917724929417	23.57372837974608
16	24.727693443763744	26.362596825035013	24.824284980264352	24.08542475093689
17	23.921990643888623	26.764849103746936	25.742425639243667	23.570734613120774
18	25.221247777297474	23.914133793482847	28.61742731173179	22.24719111748789
19	25.117823646163036	25.003653171449365	29.268074686430207	20.610448495957392
20	26.466911513941316	23.851685865572627	27.980927304379982	21.700475316106075
21	26.195254095901227	24.727146808837137	27.00417256895899	22.073426526302647
22	26.006970035091353	24.25137382612082	26.417870604388916	23.323785534398915
23	23.91310027092775	24.74802442853561	27.305719957477148	24.033155343059494
24	23.836681857677373	26.518823258205803	26.512333090986466	23.132161793130354
25	25.1938886693359	24.14484176639449	26.650023459923133	24.011246104346476
26	25.541173854358057	24.592354375374107	26.71257387972895	23.15389789053889
27	25.27491519837563	24.042510634042127	27.069527283697635	23.613046883884607
28	24.458454582491083	24.266670670539074	28.03890960052151	23.235965146448333
29	24.811983867870175	24.10271380242573	28.300920358688746	22.78438197101535
30	25.168611788747143	24.311161534218932	27.949462208240554	22.57076446879337
31	26.555387577849626	23.734103022499173	27.01768818248661	22.692821217164592
32	24.05710132296294	25.13397863766336	28.211024304960326	22.597895734413378
33	24.86547789156336	25.125065369706473	26.85517572039072	23.154281018339454
34	23.98473781338508	24.998100204043403	27.88114545582398	23.136016526747532
35	24.995866456343457	25.345105098375974	27.57604863481965	22.08297981046092
36	25.612465606111872	23.82846861940708	26.665499767230745	23.8935660072503
37	25.96306053634794	24.874512630396076	26.643301051393596	22.519125781862385
38	25.521141394580084	23.971009207314847	27.393685004678048	23.11416439342702
39	26.33590199345815	23.325406644119937	27.350091212270577	22.98860015015134
40	25.341519308979727	24.481183417489603	27.359461431386645	22.817835842144024
41	24.815729432544742	24.37579637857075	26.81511679273606	23.99335739614845
42	26.37818800671284	24.757451485569764	26.6489351643936	22.2154253433238
43	26.291852992801186	23.407659739811294	26.398717857966275	23.901769409421245
44	25.917026619789052	24.93383756315183	26.459243064377937	22.68989275268118
45	27.703850512972334	23.834912846113028	25.43079394006006	23.030442700854582
46	25.540118431955523	24.22377051766912	27.024695838973383	23.211415211401977
47	26.252249767208408	25.080237609926737	25.699754568701206	22.96775805416365
48	27.412264266698	24.56627720290612	25.23786400077734	22.783594529618544
49	26.428587213333323	25.034846761109513	26.572772150102182	21.963793875454986
50	27.674582825388516	24.824942961465528	24.02077201309242	23.479702200053538
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2369.0
1	1811.5
2	1254.0
3	1767.5
4	2281.0
5	3117.0
6	3953.0
7	3582.0
8	3211.0
9	4627.5
10	6044.0
11	10061.5
12	14079.0
13	29081.0
14	44083.0
15	55893.5
16	67704.0
17	71091.5
18	74479.0
19	68911.5
20	63344.0
21	61054.0
22	58764.0
23	57227.5
24	55691.0
25	59399.5
26	63108.0
27	66902.0
28	70696.0
29	87100.0
30	103504.0
31	117114.0
32	130724.0
33	150367.5
34	170011.0
35	198750.5
36	227490.0
37	246866.5
38	266243.0
39	307881.0
40	349519.0
41	383254.5
42	416990.0
43	453102.5
44	489215.0
45	564840.5
46	640466.0
47	697045.0
48	753624.0
49	779228.0
50	804832.0
51	731669.5
52	658507.0
53	598682.5
54	538858.0
55	517981.5
56	497105.0
57	471318.0
58	445531.0
59	417238.0
60	388945.0
61	348327.5
62	307710.0
63	271822.5
64	235935.0
65	198909.5
66	161884.0
67	137822.0
68	113760.0
69	98088.0
70	82416.0
71	67286.5
72	52157.0
73	43097.0
74	34037.0
75	28298.0
76	22559.0
77	17497.5
78	12436.0
79	10126.0
80	7816.0
81	7081.0
82	6346.0
83	5516.0
84	4686.0
85	4052.5
86	3419.0
87	2895.0
88	2371.0
89	1660.5
90	950.0
91	746.5
92	543.0
93	450.0
94	357.0
95	281.5
96	206.0
97	157.5
98	109.0
99	93.0
100	77.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030121485659265847
2	0.004998583143926816
3	4.3722831282574985E-4
4	0.004147760481130762
5	0.006842032246651599
6	0.020478828814243906
7	0.012821424849079421
8	0.016106545685986406
9	0.01621289851883591
10	0.015976558890281455
11	0.007385613392326856
12	0.010198054972124924
13	0.020396109944249848
14	0.026351868583822224
15	0.020738802405653813
16	0.04844962385366417
17	0.019119875950055765
18	0.03000331584498862
19	0.04719702382232554
20	0.035155519747475834
21	0.045140869053901744
22	0.04613349549383047
23	0.034824644267499594
24	0.040744951962788795
25	0.038889685878636296
26	0.04593260680955918
27	0.038334287751533316
28	0.04632256719667404
29	0.012088772000560598
30	0.028738898832222262
31	0.050399425789238464
32	0.04679524645378296
33	0.033347521589034215
34	0.01137975311489722
35	0.01341227392046557
36	0.01452307017467153
37	0.004620439738239681
38	0.0063693529895426804
39	0.003178768004057479
40	0.020667900517087473
41	0.0077519398165862684
42	0.011793347464867524
43	0.0036514472611663975
44	0.007657403965164484
45	0.010966158764926915
46	0.01579930416886561
47	0.011427021040608111
48	0.03228399326053915
49	0.004124126518275316
50	0.011970602186283368
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	8462398.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.64888151675178
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.0595525316986	41.545285139861384
2	4.795845209388337	4.474416298908763
3	1.911291756453357	2.674788680722112
4	1.0950573436610316	2.043328011139696
5	0.6699540516387495	1.5626303588281918
6	0.45032276139205624	1.2604231884284707
7	0.305576987501114	0.9978377278929785
8	0.23594734318151492	0.8805343725013489
9	0.17531131288487076	0.7360268996981282
>10	1.0434758620721847	9.153747393234122
>50	0.11265290895238764	3.695766726222528
>100	0.11670271228056583	11.479336191732123
>500	0.01682252162076842	5.4023599232126855
>1k	0.010525467384840894	8.931862561314366
>5k	6.247994283978068E-4	1.9493423289316505
>10k+	3.3643046144497287E-4	3.2123141973715756
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	54945	0.6492840445462386	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27100	0.3202401966912925	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	22996	0.2717433049119174	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	22905	0.27066795960199463	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	21604	0.25529406676452704	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20646	0.24397339855676842	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	20006	0.2364105304430257	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	19365	0.2288358453478553	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18062	0.21343831854753226	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	13645	0.1612427115812799	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	12981	0.15339623591327187	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	11973	0.14148471863412712	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	10283	0.1215140200212753	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	10045	0.11870157844147723	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	9912	0.11712991991159007	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	9713	0.1147783406074732	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	9081	0.10731000834515228	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	8936	0.10559654603813245	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4815660998218236E-4	0.0	0.0	1.181698142772297E-5	0.0
2	4.4904529425347285E-4	0.0	0.0	9.453585142178376E-5	0.0
3	5.435811456752566E-4	0.0	0.0	1.0635283284950673E-4	0.0
4	5.435811456752566E-4	1.181698142772297E-5	0.0	1.0635283284950673E-4	0.0
5	5.908490713861485E-4	1.181698142772297E-5	0.0	1.2998679570495265E-4	0.0
6	6.263000156693174E-4	1.181698142772297E-5	0.0	1.4180377713267562E-4	0.0
7	6.735679413802093E-4	1.181698142772297E-5	0.0	1.8907170284356752E-4	0.0
8	0.001110796254205959	1.181698142772297E-5	0.0	4.608622756811958E-4	0.0
9	0.001110796254205959	1.181698142772297E-5	0.0	4.608622756811958E-4	0.0
10	0.005743052973873363	1.181698142772297E-5	0.0	0.00855549455367143	0.0
11	0.005766686936728809	1.181698142772297E-5	0.0	0.008567311535099154	0.0
12	0.006463888840964464	1.181698142772297E-5	0.0	0.009063624755063518	0.0
13	0.006499339785247633	2.363396285544594E-5	0.0	0.009063624755063518	0.0
14	0.006499339785247633	2.363396285544594E-5	0.0	0.009087258717918963	0.0
15	0.006593875636669417	2.363396285544594E-5	0.0	0.009205428532196193	1.181698142772297E-5
16	0.006617509599524863	2.363396285544594E-5	0.0	0.009217245513623917	2.363396285544594E-5
17	0.00808281529656251	2.363396285544594E-5	0.0	0.010339858749257597	2.363396285544594E-5
18	0.008260070017978356	2.363396285544594E-5	0.0	0.010564381396384335	2.363396285544594E-5
19	0.008342788887972416	2.363396285544594E-5	0.0	0.010765270080655625	2.363396285544594E-5
20	0.008567311535099154	2.363396285544594E-5	0.0	0.010966158764926915	2.363396285544594E-5
21	0.009039990792208071	2.363396285544594E-5	0.0	0.011651543687734849	2.363396285544594E-5
22	0.010363492712113044	2.363396285544594E-5	0.0	0.01396767204756855	2.363396285544594E-5
23	0.011214315374909098	2.363396285544594E-5	0.0	0.017879092900144854	2.363396285544594E-5
24	0.01201787011199426	2.363396285544594E-5	0.0	0.020065234464273603	2.363396285544594E-5
25	0.01201787011199426	2.363396285544594E-5	0.0	0.020124319371412215	2.363396285544594E-5
26	0.012076955019132874	2.363396285544594E-5	0.0	0.020396109944249844	2.363396285544594E-5
27	0.012088772000560598	3.5450944283168905E-5	0.0	0.020561547684237968	2.363396285544594E-5
28	0.012112405963416043	3.5450944283168905E-5	0.0	0.021057860904202332	2.363396285544594E-5
29	0.012112405963416043	3.5450944283168905E-5	0.0	0.021967768474137	2.363396285544594E-5
30	0.012112405963416043	3.5450944283168905E-5	0.0	0.023102198691198405	2.363396285544594E-5
31	0.012443281443392287	3.5450944283168905E-5	0.0	0.024602955332519223	2.363396285544594E-5
32	0.012514183331958624	3.5450944283168905E-5	0.0	0.0260328100852737	2.363396285544594E-5
33	0.0126559871090913	3.5450944283168905E-5	0.0	0.027958978057992544	2.363396285544594E-5
34	0.01277415692336853	3.5450944283168905E-5	0.0	0.03015693660354902	3.5450944283168905E-5
35	0.012880509756218036	4.726792571089188E-5	0.0	0.032780306480503514	3.5450944283168905E-5
36	0.012951411644784375	4.726792571089188E-5	0.0	0.03564001598601248	3.5450944283168905E-5
37	0.01297504560763982	4.726792571089188E-5	0.0	0.03979959344857096	3.5450944283168905E-5
38	0.01297504560763982	4.726792571089188E-5	0.0	0.044691823759648266	3.5450944283168905E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATTCG	2700	0.0	23.795748	15
AATTCGT	2660	0.0	23.078114	16
GCATATA	820	0.0	22.53691	1
CAATGCG	2855	0.0	21.80883	19
AATGCGA	3000	0.0	21.415033	20
TCGTCTC	2805	0.0	21.413212	19
ATCGTCA	3030	0.0	21.346968	10
ATTCGTC	2925	0.0	21.28691	17
GTACTAG	3045	0.0	21.097183	1
TAGGCAT	3230	0.0	21.04388	5
GTGATCG	3285	0.0	20.959152	8
GTTAATA	3230	0.0	20.638067	1
TGCGACG	3055	0.0	20.384697	22
TGATCGC	3415	0.0	20.356337	9
GGCAATT	3255	0.0	20.346544	13
CGACGAG	3175	0.0	19.891603	24
AGTCCTA	3140	0.0	19.339	29
GGTATAG	2470	0.0	19.328215	1
CGTCTCC	3260	0.0	19.302147	20
TTAATAA	3395	0.0	19.241905	2
>>END_MODULE