FastQCFastQC Report
Wed 25 May 2016
SRR2558176_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2558176_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13874440
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA573590.41341488377188557No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT447480.3225211251769441No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT444080.32007057582143855No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC278400.20065674722727547No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271750.19586376098783084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229970.1657508339075307No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG228630.16478502916153734No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA218240.15729643863103662No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG217470.15674146127699567No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG198860.14332830730465518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG24600.019.1442971
TGCGACG21000.018.86191422
CATTGCG30150.018.53273629
AGGTCGT29450.018.30683722
GCTCGTC30550.018.29172939
AATGCGA22400.018.26951420
CAATGCG22400.018.26878719
CGACGAA26150.017.9199638
CGTCATT31900.017.79715526
CGACGAG23650.017.67927424
GTCAGCG30400.017.65771343
GTCGTCA31050.017.43473824
TAGGTCG31900.016.89875421
TCGACGA27400.016.86053337
GTCGACG27800.016.5389236
TCAGCGC33050.016.24253544
TCGTCAG34300.016.0334941
CGAACAA29950.016.01186241
CAATTCG29500.015.808866515
CCGTCGG42050.015.64757924
Produced by FastQC (version 0.11.5)