##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2558176_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13874440
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.553497438455175	31.0	30.0	34.0	26.0	34.0
2	30.678904590023095	31.0	30.0	34.0	26.0	34.0
3	30.89755961321682	31.0	30.0	34.0	27.0	34.0
4	34.53367126889446	35.0	35.0	37.0	30.0	37.0
5	34.131829464828854	35.0	35.0	37.0	30.0	37.0
6	34.17628488068708	36.0	35.0	37.0	30.0	37.0
7	34.03618423518355	36.0	35.0	37.0	29.0	37.0
8	34.02176772540009	36.0	35.0	37.0	29.0	37.0
9	35.42900938704553	38.0	35.0	39.0	28.0	39.0
10	35.33105948780636	38.0	35.0	39.0	28.0	39.0
11	35.41889762757992	38.0	35.0	39.0	28.0	39.0
12	35.27039426456131	38.0	35.0	39.0	27.0	39.0
13	35.30538342448416	38.0	35.0	39.0	27.0	39.0
14	36.21237347237078	39.0	36.0	40.0	27.0	41.0
15	36.19660433141806	39.0	36.0	40.0	27.0	41.0
16	36.17091608742407	39.0	36.0	40.0	27.0	41.0
17	36.127699712564976	39.0	35.0	40.0	27.0	41.0
18	36.10828912734496	39.0	35.0	40.0	27.0	41.0
19	36.15398689965144	39.0	35.0	40.0	27.0	41.0
20	36.12099219860405	39.0	35.0	40.0	27.0	41.0
21	36.05101005878436	39.0	35.0	40.0	27.0	41.0
22	35.993794127907144	39.0	35.0	40.0	27.0	41.0
23	35.75824141370751	39.0	34.0	40.0	27.0	41.0
24	35.72199115784132	39.0	34.0	40.0	27.0	41.0
25	35.708932756925684	39.0	34.0	40.0	27.0	41.0
26	35.639846437045385	39.0	34.0	40.0	26.0	41.0
27	35.43933347940529	38.0	34.0	40.0	25.0	41.0
28	35.43249803235302	38.0	34.0	40.0	25.0	41.0
29	35.40495659644642	38.0	34.0	40.0	25.0	41.0
30	35.309364702287084	38.0	34.0	40.0	25.0	41.0
31	35.208072902401824	38.0	34.0	40.0	25.0	41.0
32	35.07604155555107	38.0	34.0	40.0	25.0	41.0
33	34.94350762985749	38.0	33.0	40.0	25.0	41.0
34	34.828547530567	38.0	33.0	40.0	24.0	41.0
35	34.74937229899009	38.0	33.0	40.0	24.0	41.0
36	34.59853875183431	38.0	33.0	40.0	24.0	41.0
37	34.5448140609639	38.0	33.0	40.0	24.0	41.0
38	34.46555111413506	38.0	33.0	40.0	23.0	41.0
39	34.3923250956435	38.0	33.0	40.0	23.0	41.0
40	34.26861473327933	38.0	32.0	40.0	23.0	41.0
41	34.14404401186643	38.0	32.0	40.0	22.0	41.0
42	34.063286950680535	38.0	32.0	40.0	22.0	41.0
43	33.96402730488582	38.0	32.0	40.0	22.0	41.0
44	33.78466439005827	37.0	31.0	40.0	21.0	41.0
45	33.65632681391105	37.0	31.0	40.0	20.0	41.0
46	33.45305403317179	37.0	31.0	40.0	20.0	41.0
47	33.30121374268079	37.0	31.0	40.0	19.0	41.0
48	33.15536627063867	37.0	30.0	40.0	18.0	41.0
49	33.05812912088704	37.0	30.0	40.0	18.0	41.0
50	31.76937209718014	35.0	28.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	31.0
5	105.0
6	186.0
7	336.0
8	715.0
9	1891.0
10	4894.0
11	11869.0
12	24718.0
13	42067.0
14	59105.0
15	71956.0
16	81561.0
17	88557.0
18	93745.0
19	99222.0
20	104327.0
21	110589.0
22	118072.0
23	128151.0
24	141332.0
25	157530.0
26	179148.0
27	203839.0
28	234995.0
29	274943.0
30	327840.0
31	400459.0
32	498558.0
33	632743.0
34	818800.0
35	1067380.0
36	1398705.0
37	1845253.0
38	2287513.0
39	2363302.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.21637775035808	19.965012485960866	13.474266922096719	24.344342841584336
2	15.430590480987252	21.80243312846443	37.43815678961564	25.328819600932682
3	17.539850518928183	27.690895712978815	29.166706310317814	25.602547457775188
4	12.854010714036262	18.256252719721612	36.377727623534376	32.51200894270775
5	13.60650424368148	38.373493567687035	33.754738610645774	14.265263577985712
6	30.997199292346554	37.20507169900994	17.89147218189156	13.906256826751942
7	27.412807795975752	31.88379510404268	22.789119643079978	17.91427745690159
8	27.77006667611609	32.711130099678925	20.20329142758927	19.315511796615713
9	27.751078065370816	14.437172593695227	19.765514654018443	38.04623468691551
10	16.987804943094204	27.66633256407569	31.581545946938665	23.76431654589144
11	33.63756887950811	23.656033330286917	22.823972620314443	19.88242516989053
12	23.762314709379886	25.369395322582218	29.376866964191418	21.49142300384648
13	29.16261634333037	21.3071394724486	25.346112532938985	24.184131651282044
14	23.53888829939991	20.3931460565302	26.868676048768165	29.19928959530172
15	25.03433546619036	27.125097578749564	23.935427555301832	23.905139399758244
16	23.486007185457474	26.98887986195641	25.528282782631585	23.99683016995453
17	22.745377526010312	26.977129146415358	26.68486587317915	23.592627454395178
18	23.557460261205584	25.887152918833007	28.180302084694826	22.37508473526659
19	23.787092638453615	26.14965407502846	28.419664144801725	21.6435891417162
20	24.461973645734865	25.38824295478756	27.914619425009384	22.23516397446819
21	24.60607790848316	25.6986177527181	27.113141262024282	22.582163076774453
22	24.497908171516166	25.28718436288363	26.84828891984476	23.366618545755443
23	23.531484324616997	25.634947728296005	27.287354324685488	23.54621362240151
24	23.502924044917506	26.594027371650412	26.76710648836143	23.13594209507065
25	23.921849865729435	25.165425388893603	26.988863146556774	23.92386159882019
26	23.877356062370268	25.46238771686084	27.139899077654256	23.520357143114634
27	24.07509398824881	25.29559840476825	26.941010094747075	23.688297512235863
28	23.447614000364123	25.253017086760508	27.869002103623746	23.43036680925162
29	23.59519704885332	24.981347381140598	28.174958285240653	23.248497284765428
30	23.8112335013877	25.772635874062445	27.57969127433958	22.836439350210274
31	24.5032501427525	24.962754575289686	27.129081423718254	23.40491385823956
32	23.096323377627463	25.639956874638454	27.7396973596351	23.52402238809898
33	23.760401488649254	25.77725205704975	27.055451225990335	23.406895228310663
34	23.164222586835486	25.771691193484365	27.58279810294098	23.481288116739165
35	23.82169300216296	26.118260826440036	27.498738886232182	22.56130728516482
36	24.05771990668405	25.58643428362752	26.650053872998992	23.70579193668944
37	24.21161421501574	25.77277654041908	26.90305019271344	23.11255905185174
38	24.198397356974013	25.57881069597416	26.905809328673016	23.316982618378812
39	24.087128035594787	25.2215409766995	27.22190203474376	23.46942895296195
40	24.02803257010017	25.37390793335344	27.38549772402613	23.21256177252026
41	23.292599038140008	25.556435047203134	27.2960985194674	23.854867395189462
42	24.310523281287644	26.011001120094647	26.741182011992176	22.937293586625533
43	24.501175876818767	24.9007440600507	26.738740403721433	23.859339659409102
44	23.971461992486688	25.81019286241228	27.19973144498862	23.018613700112418
45	24.907849416011985	25.14675753648033	26.48181282106365	23.46358022644404
46	23.839152142980357	25.33804140743677	27.415319074887083	23.407487374695787
47	24.24291002802096	25.89727406523635	26.62241391415065	23.23740199259204
48	24.868420047128424	25.99402467040309	25.95001208994225	23.18754319252624
49	24.371447674916467	25.8050927723943	26.947848343019558	22.875611209669678
50	24.62108026966195	25.958714546499746	25.673778240709872	23.746426943128434
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2150.0
1	1947.5
2	1745.0
3	3239.5
4	4734.0
5	5619.5
6	6505.0
7	5433.5
8	4362.0
9	6365.5
10	8369.0
11	13418.5
12	18468.0
13	37394.0
14	56320.0
15	73689.5
16	91059.0
17	91290.0
18	91521.0
19	87210.5
20	82900.0
21	89379.0
22	95858.0
23	108875.0
24	121892.0
25	145780.0
26	169668.0
27	193159.0
28	216650.0
29	250656.0
30	284662.0
31	323345.0
32	362028.0
33	399745.5
34	437463.0
35	485284.5
36	533106.0
37	565729.5
38	598353.0
39	639776.5
40	681200.0
41	716362.5
42	751525.0
43	780224.0
44	808923.0
45	872036.5
46	935150.0
47	991443.5
48	1047737.0
49	1061304.0
50	1074871.0
51	986553.5
52	898236.0
53	839404.5
54	780573.0
55	750352.5
56	720132.0
57	692436.5
58	664741.0
59	633543.5
60	602346.0
61	532589.5
62	462833.0
63	410225.0
64	357617.0
65	309874.5
66	262132.0
67	225932.5
68	189733.0
69	166894.5
70	144056.0
71	120697.0
72	97338.0
73	85677.5
74	74017.0
75	63862.0
76	53707.0
77	42781.0
78	31855.0
79	24959.5
80	18064.0
81	14623.0
82	11182.0
83	9220.5
84	7259.0
85	5945.0
86	4631.0
87	3735.0
88	2839.0
89	2140.5
90	1442.0
91	1221.5
92	1001.0
93	796.5
94	592.0
95	522.0
96	452.0
97	362.0
98	272.0
99	221.5
100	171.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04370626850525138
2	2.810924260726919E-4
3	0.006083128400137231
4	0.008915675155177435
5	0.019878279772012418
6	0.03152559670876807
7	0.02070714205402164
8	0.029593987216781362
9	0.02375591375219468
10	0.0033010341318280238
11	0.0015279895981387358
12	0.023964931197223097
13	0.007654362986902535
14	0.01285817661829955
15	0.007423723047560838
16	0.008223755337152347
17	0.01206535182681247
18	0.023763121250299113
19	0.023236973888675867
20	0.026984872902978428
21	0.025586618270719393
22	0.03255626893770127
23	0.029730929680765496
24	0.04063587431276506
25	0.04181069650378682
26	0.03149676671635035
27	0.03138865424478393
28	0.039749352045920415
29	0.007589495503962682
30	0.008303037816301054
31	0.030848091886951833
32	0.03680869281931379
33	0.016339398202738272
34	0.011517581970875941
35	0.012843761622090695
36	0.01440778871075157
37	0.009016580128639427
38	0.013377116481818366
39	0.029507497239528228
40	0.018566515117006525
41	0.011005849605461554
42	0.01728358045441834
43	0.006940820674564163
44	0.03355811117421676
45	0.018595345109424236
46	0.015142953517403225
47	0.00903820262295271
48	0.024548738543681763
49	0.008158887854212494
50	0.010919359628208418
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1.387444E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.661411623476866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.67441841247287	25.203495262570275
2	11.506057719570912	8.206445210022036
3	5.815662008914321	6.221841502887299
4	3.374451346191073	4.8135079383966195
5	2.1403504923170225	3.816395996251435
6	1.4270730691775217	3.0534864082030846
7	0.9593594195432135	2.3948477808633255
8	0.7108318772954764	2.0279414537058225
9	0.5389514443817465	1.7297792372848373
>10	2.5471971808619007	15.670720854558079
>50	0.15494453723813217	3.830927624719664
>100	0.1238169460628687	8.918414001196554
>500	0.016277739054432718	4.026102487068566
>1k	0.009825768345049857	6.213368903547909
>5k	4.210976935161632E-4	0.9908032794141245
>10k+	3.6094088015671137E-4	2.8819220593103436
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	57359	0.41341488377188557	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44748	0.3225211251769441	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	44408	0.32007057582143855	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	27840	0.20065674722727547	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27175	0.19586376098783084	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22997	0.1657508339075307	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	22863	0.16478502916153734	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	21824	0.15729643863103662	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	21747	0.15674146127699567	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	19886	0.14332830730465518	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1531996967084798E-4	0.0	0.0	0.0	0.0
2	2.1622494313283997E-4	0.0	0.0	7.207498104427999E-6	0.0
3	3.171299165948319E-4	7.207498104427999E-6	0.0	4.324498862656799E-5	0.0
4	3.171299165948319E-4	7.207498104427999E-6	0.0	8.648997725313598E-5	0.0
5	4.1803489005682394E-4	2.8829992417711996E-5	0.0	8.648997725313598E-5	0.0
6	4.2524238816125194E-4	2.8829992417711996E-5	0.0	8.648997725313598E-5	0.0
7	4.756948748922479E-4	2.8829992417711996E-5	0.0	8.648997725313598E-5	0.0
8	6.847123199206598E-4	2.8829992417711996E-5	0.0	1.0090497346199199E-4	0.0
9	7.063348142339438E-4	2.8829992417711996E-5	0.0	2.0180994692398397E-4	0.0
10	0.006486748293985199	2.8829992417711996E-5	0.0	3.387524109081159E-4	0.0
11	0.00662369075796933	2.8829992417711996E-5	0.0	3.459599090125439E-4	0.0
12	0.007164253115801431	2.8829992417711996E-5	0.0	3.531674071169719E-4	0.0
13	0.007207498104427999	2.8829992417711996E-5	0.0	3.675824033258279E-4	0.0
14	0.007221913100636855	2.8829992417711996E-5	0.0	3.747899014302559E-4	0.0
15	0.00730840307788999	2.8829992417711996E-5	0.0	4.2524238816125194E-4	0.0
16	0.007315610575994418	2.8829992417711996E-5	0.0	4.4686488247453593E-4	3.603749052213999E-5
17	0.00817330285042135	2.8829992417711996E-5	0.0	5.045248673099599E-4	3.603749052213999E-5
18	0.008288622820092198	2.8829992417711996E-5	0.0	5.261473616232439E-4	3.603749052213999E-5
19	0.008360697801136479	2.8829992417711996E-5	0.0	5.477698559365279E-4	4.324498862656799E-5
20	0.008432772782180758	2.8829992417711996E-5	0.0	6.342598331896638E-4	5.765998483542399E-5
21	0.008793147687402159	2.8829992417711996E-5	0.0	6.991273161295158E-4	7.207498104427998E-5
22	0.00990310239548407	2.8829992417711996E-5	0.0	9.153522592623559E-4	7.207498104427998E-5
23	0.01080403965853757	6.486748293985198E-5	0.0	0.0013045571569014678	7.928247914870798E-5
24	0.011503166974667085	6.486748293985198E-5	0.0	0.0016649320621228677	7.928247914870798E-5
25	0.011546411963293654	6.486748293985198E-5	0.0	0.0017225920469582916	7.928247914870798E-5
26	0.011575241955711366	6.486748293985198E-5	0.0	0.0019532319862999876	8.648997725313598E-5
27	0.011589656951920222	6.486748293985198E-5	0.0	0.0020973819483885475	1.0811247156641998E-4
28	0.011632901940546789	6.486748293985198E-5	0.0	0.0025514543289675115	1.1531996967084798E-4
29	0.01166893943106893	6.486748293985198E-5	0.0	0.0035821265579007154	1.1531996967084798E-4
30	0.011733806914008781	6.486748293985198E-5	0.0	0.004778571243235763	1.1531996967084798E-4
31	0.012058144328708041	6.486748293985198E-5	0.0	0.006126373388763799	1.2252746777527597E-4
32	0.012151841804065606	6.486748293985198E-5	0.0	0.007582288005858254	1.2252746777527597E-4
33	0.012223916785109885	6.486748293985198E-5	0.0	0.009477860007322819	1.2252746777527597E-4
34	0.012476179218764866	6.486748293985198E-5	0.0	0.011604071948129078	1.4414996208855996E-4
35	0.012584291690331286	6.486748293985198E-5	0.0	0.014487071189900277	1.4414996208855996E-4
36	0.012613121682748998	7.207498104427998E-5	0.0	0.0172475429638962	1.5135746019298796E-4
37	0.012634744177062281	7.207498104427998E-5	0.0	0.021377439377733443	1.5135746019298796E-4
38	0.01264195167516671	9.369747535756397E-5	0.0	0.025918163183523083	1.6577245640184396E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	2460	0.0	19.144297	1
TGCGACG	2100	0.0	18.861914	22
CATTGCG	3015	0.0	18.532736	29
AGGTCGT	2945	0.0	18.306837	22
GCTCGTC	3055	0.0	18.291729	39
AATGCGA	2240	0.0	18.269514	20
CAATGCG	2240	0.0	18.268787	19
CGACGAA	2615	0.0	17.91996	38
CGTCATT	3190	0.0	17.797155	26
CGACGAG	2365	0.0	17.679274	24
GTCAGCG	3040	0.0	17.657713	43
GTCGTCA	3105	0.0	17.434738	24
TAGGTCG	3190	0.0	16.898754	21
TCGACGA	2740	0.0	16.860533	37
GTCGACG	2780	0.0	16.53892	36
TCAGCGC	3305	0.0	16.242535	44
TCGTCAG	3430	0.0	16.03349	41
CGAACAA	2995	0.0	16.011862	41
CAATTCG	2950	0.0	15.8088665	15
CCGTCGG	4205	0.0	15.647579	24
>>END_MODULE