FastQCFastQC Report
Wed 25 May 2016
SRR2558175_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2558175_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15143528
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA648510.42824234881066026No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT579330.3825594669881417No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC459500.3034299537069565No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT432620.28567979667617743No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT362050.2390790309893441No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA337730.22301936510435347No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304210.2008844966641855No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG297030.19614319727873186No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG249400.16469081709361255No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG230610.1522828762227666No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA220170.1453888420188479No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA204390.13496854894051108No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT200160.13217527646133714No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA179950.11882964128306164No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTT169880.11217993587755773No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC165450.10925459377761906No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC163150.10773579313882473No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT158720.10481045103888605No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG154860.10226150735812686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGT45850.014.8240749
CGTCGTA45750.014.80712410
CAATGCG11550.014.66730819
AATGCGA11400.014.47455620
TGCGACG11200.014.34242422
TACCGTC48600.014.2128757
CGACGAA12350.014.07223338
GTCGACG11750.014.04070936
GCATTCG84300.014.017302522
GTACTAG16350.013.9984261
GGCGTTA48650.013.97324342
ATTCGTA84050.013.90237124
GACCGTC20150.013.86903922
ATACCGT51600.013.8565546
CCGTCGG20350.013.84132424
TCGACGA12150.013.75946337
CGACGAG12750.013.63480624
GACGGCC89650.013.56778311
GTCGTAG50600.013.5189811
CGAGCCG68600.013.46895315
Produced by FastQC (version 0.11.5)