##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2558175_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15143528
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.630604440391963	31.0	30.0	34.0	26.0	34.0
2	30.752298011401304	31.0	30.0	34.0	27.0	34.0
3	30.972274558478052	31.0	31.0	34.0	27.0	34.0
4	34.61599047461067	37.0	35.0	37.0	31.0	37.0
5	34.22839288176441	35.0	35.0	37.0	30.0	37.0
6	34.25929690888411	37.0	35.0	37.0	30.0	37.0
7	34.12471908791663	36.0	35.0	37.0	29.0	37.0
8	34.109312044062655	36.0	35.0	37.0	29.0	37.0
9	35.52036143757254	38.0	35.0	39.0	29.0	39.0
10	35.42966381413895	38.0	35.0	39.0	28.0	39.0
11	35.51029073278037	38.0	35.0	39.0	29.0	39.0
12	35.37193955067802	38.0	35.0	39.0	28.0	39.0
13	35.40077701840681	38.0	35.0	39.0	28.0	39.0
14	36.30877441505044	39.0	36.0	40.0	28.0	41.0
15	36.27876370684559	39.0	36.0	40.0	27.0	41.0
16	36.26223393914549	39.0	36.0	40.0	27.0	41.0
17	36.223879336439964	39.0	36.0	40.0	27.0	41.0
18	36.20803917026468	39.0	36.0	40.0	27.0	41.0
19	36.24838888269629	39.0	35.0	41.0	27.0	41.0
20	36.21567886954744	39.0	35.0	41.0	27.0	41.0
21	36.150498351507	39.0	35.0	40.0	27.0	41.0
22	36.097498152346006	39.0	35.0	40.0	27.0	41.0
23	35.84085775784877	39.0	34.0	40.0	27.0	41.0
24	35.8119510856387	39.0	34.0	40.0	27.0	41.0
25	35.804006371566786	39.0	34.0	40.0	27.0	41.0
26	35.73067200720995	39.0	34.0	40.0	27.0	41.0
27	35.52911494600202	39.0	34.0	40.0	25.0	41.0
28	35.50312536154059	38.0	34.0	40.0	25.0	41.0
29	35.462891011922714	38.0	34.0	40.0	25.0	41.0
30	35.35376663879117	38.0	34.0	40.0	25.0	41.0
31	35.26011514621956	38.0	34.0	40.0	25.0	41.0
32	35.119984127872975	38.0	34.0	40.0	25.0	41.0
33	34.98052336285178	38.0	33.0	40.0	25.0	41.0
34	34.8845856130751	38.0	33.0	40.0	24.0	41.0
35	34.795413525830966	38.0	33.0	40.0	24.0	41.0
36	34.62426437221234	38.0	33.0	40.0	24.0	41.0
37	34.58537138769777	38.0	33.0	40.0	24.0	41.0
38	34.491174975870884	38.0	33.0	40.0	23.0	41.0
39	34.43045827894266	38.0	33.0	40.0	23.0	41.0
40	34.28860144082673	38.0	33.0	40.0	23.0	41.0
41	34.14996300729922	38.0	32.0	40.0	22.0	41.0
42	34.06414502617884	38.0	32.0	40.0	22.0	41.0
43	33.96633175571769	37.0	32.0	40.0	22.0	41.0
44	33.774897500767324	37.0	31.0	40.0	21.0	41.0
45	33.67585208677925	37.0	31.0	40.0	20.0	41.0
46	33.44666170260985	37.0	31.0	40.0	20.0	41.0
47	33.30630326037631	37.0	31.0	40.0	19.0	41.0
48	33.17123031040059	37.0	30.0	40.0	19.0	41.0
49	33.0699237324354	37.0	30.0	40.0	18.0	41.0
50	31.820040548014966	35.0	28.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	30.0
5	94.0
6	172.0
7	322.0
8	655.0
9	1995.0
10	5449.0
11	12981.0
12	26551.0
13	44403.0
14	62377.0
15	75935.0
16	86021.0
17	93946.0
18	100124.0
19	104772.0
20	111164.0
21	116581.0
22	125243.0
23	136257.0
24	150779.0
25	169090.0
26	192075.0
27	219356.0
28	253338.0
29	295366.0
30	353312.0
31	431305.0
32	539240.0
33	685275.0
34	893171.0
35	1169236.0
36	1543709.0
37	2037253.0
38	2520660.0
39	2585287.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.163643942892286	20.18114541775423	13.86178644263299	23.79342419672049
2	15.230430206955747	22.600711500970153	37.18182591992236	24.987032372151734
3	17.12326894520284	28.20339325108703	29.832903707846036	24.84043409586409
4	12.375311652763958	18.42550114570137	36.77151610133578	32.42767110019889
5	13.515369258418577	38.65366032017915	33.33301190413053	14.497958517271744
6	30.465422924876123	37.720852724689166	18.126952236362552	13.686772114072166
7	26.843789387418337	32.58060205952751	22.87581297877086	17.699795574283296
8	27.657348883932226	32.31277243800607	20.646368664602623	19.38351001345908
9	27.653584312059483	14.359936349115014	20.2825307224106	37.70394861641491
10	17.06643529101236	27.86195463516566	30.77878427537489	24.29282579844709
11	33.401979603829616	23.92788958679044	23.197396504455128	19.472734304924813
12	23.641704696420913	26.001810527611735	29.159520745177552	21.196964030789804
13	28.27469280355832	21.4084953879259	26.0128956862136	24.303916122302184
14	23.81661334919594	21.184688024607027	26.71008145655594	28.288617169641096
15	24.763095169461423	27.514092315039957	24.409064490324663	23.313748025173958
16	23.45012866532395	27.393258895923005	25.818460842300546	23.3381515964525
17	22.36380089230687	27.29733752937879	27.01957141902364	23.3192901592907
18	23.375922142543693	25.796233412794635	28.944622174854846	21.88322226980683
19	23.778855626180423	26.274449323640404	28.580832080326307	21.365862969852866
20	24.61523981068386	25.033370751376093	28.010089991548742	22.341299446391304
21	25.36071878894921	25.537153127066148	27.20521456204165	21.896913521943
22	24.423321400814398	25.509917351332206	27.213488427455886	22.853272820397514
23	22.637129900084275	26.147506327669966	27.856431329547508	23.35893244269825
24	23.126420571932737	26.90802624339329	27.389746574624407	22.575806610049565
25	23.55628541731708	25.079017833726898	27.175654302543013	24.189042446413005
26	23.342641593284526	25.846294021853932	27.921364887356976	22.889699497504566
27	23.98207533111533	25.70622260081421	27.09329647345793	23.21840559461253
28	23.189830765098502	25.37564340191575	28.576260343743986	22.858265489241766
29	23.32023860295521	25.09834255661458	28.843786334046516	22.737632506383697
30	23.474301461218825	25.685152491761688	28.561481074382133	22.279064972637354
31	24.06008616008973	25.26908184415883	27.767803710353085	22.90302828539836
32	22.97180777750878	25.548567070683514	27.985985304254513	23.49363984755319
33	22.88153201395497	26.01448544085332	27.824769279621954	23.279213265569762
34	22.947876052391152	25.964905699703326	28.094777249899057	22.992440998006465
35	24.00756399598144	25.861678175257623	28.081807285982872	22.048950542778066
36	23.350152127322403	26.10937303624526	27.17098524574	23.369489590692343
37	24.089125494831066	26.085204159027743	27.48402173541926	22.341648610721933
38	23.612218246295228	25.445125581160667	27.6997099793801	23.24294619316401
39	23.900050787845714	25.263039625814653	27.49876167884313	23.338147907496502
40	23.99494454781066	25.801629376784614	27.49271327077995	22.710712804624777
41	22.522493964164195	26.374109417858676	27.714567540405	23.38882907757213
42	24.669296662737874	26.361485941616042	27.14473885647479	21.824478539171295
43	24.311858658027884	24.873777989396118	27.241718955804384	23.572644396771615
44	23.513043946968924	26.01041724327761	27.402877358534187	23.073661451219277
45	24.54267576853752	25.555907138091953	26.843584231833656	23.057832861536866
46	23.01499877257373	25.996716692515154	28.108741844153652	22.879542690757464
47	23.872200945919523	25.872240173770134	27.45026301481353	22.805295865496813
48	24.777786085471014	26.424188879251375	26.23832259042917	22.55970244484844
49	23.47630005544059	26.48870864368012	27.43570922186043	22.599282079018856
50	24.369251532709978	26.512710927791368	25.868065656710083	23.249971882788575
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4741.0
1	3840.0
2	2939.0
3	4935.5
4	6932.0
5	7916.0
6	8900.0
7	7216.5
8	5533.0
9	8130.0
10	10727.0
11	17057.5
12	23388.0
13	47895.0
14	72402.0
15	94063.5
16	115725.0
17	115619.5
18	115514.0
19	108921.0
20	102328.0
21	116808.5
22	131289.0
23	150944.0
24	170599.0
25	195110.0
26	219621.0
27	251297.0
28	282973.0
29	322048.5
30	361124.0
31	399702.0
32	438280.0
33	473523.0
34	508766.0
35	551070.5
36	593375.0
37	637881.5
38	682388.0
39	718393.0
40	754398.0
41	792627.0
42	830856.0
43	831600.0
44	832344.0
45	893171.0
46	953998.0
47	1057222.5
48	1160447.0
49	1153791.5
50	1147136.0
51	1016132.0
52	885128.0
53	822466.0
54	759804.0
55	770909.0
56	782014.0
57	751158.0
58	720302.0
59	668555.0
60	616808.0
61	551889.5
62	486971.0
63	425785.5
64	364600.0
65	315002.5
66	265405.0
67	237945.0
68	210485.0
69	187623.5
70	164762.0
71	134104.0
72	103446.0
73	94794.5
74	86143.0
75	74567.0
76	62991.0
77	47853.0
78	32715.0
79	26053.5
80	19392.0
81	16920.0
82	14448.0
83	12574.0
84	10700.0
85	9391.0
86	8082.0
87	6638.5
88	5195.0
89	3925.0
90	2655.0
91	2200.0
92	1745.0
93	1462.0
94	1179.0
95	1032.5
96	886.0
97	720.0
98	554.0
99	474.5
100	395.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.043041489407224
2	3.2357057087357716E-4
3	0.005824270275724389
4	0.008419438323751242
5	0.01860200608471157
6	0.029583595051298484
7	0.020239669382194164
8	0.0284213823885689
9	0.022715974771532765
10	0.0031498604552387
11	0.0012876788024560723
12	0.021065104511973694
13	0.0072638291420598945
14	0.012130594667240025
15	0.006966672495339263
16	0.007541175345665819
17	0.010908950675166316
18	0.021421692488038456
19	0.021679228248529667
20	0.024914933957265437
21	0.02389139439633882
22	0.029755285558292627
23	0.026869564344583378
24	0.037633238436908495
25	0.038339810907999775
26	0.028302519729880647
27	0.02900248871993369
28	0.03720401216942314
29	0.006933655090148082
30	0.007719469333698198
31	0.028183657071192393
32	0.033895668169266764
33	0.01489745322226102
34	0.010446707002489775
35	0.011859851944672338
36	0.012777735808987178
37	0.008181713006374736
38	0.012190025996584152
39	0.026935599154965736
40	0.017479414308211403
41	0.00968070320205437
42	0.015280455122478727
43	0.006359152239821527
44	0.03157784632484584
45	0.0162775807592524
46	0.013794671888875563
47	0.008089264271839428
48	0.0238517735101094
49	0.007877952878615868
50	0.01028161997653387
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1.5143528E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.62207373649138
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.04958345566264	28.213263986445835
2	12.815941365388051	9.899564648331346
3	5.551992372143263	6.432883763440644
4	2.824350746136378	4.363291310999745
5	1.6144127057520594	3.11759832813423
6	0.9631872981575019	2.232017451089457
7	0.6358369095450191	1.7190138003381368
8	0.44707533613700545	1.3813581278440077
9	0.32250208705568106	1.1210129447793187
>10	1.536179309276335	10.566158615998233
>50	0.11785890496400658	3.175230194405513
>100	0.09384416176772882	7.467668215768759
>500	0.014085823187229044	3.7803697932154336
>1k	0.011526985149717204	9.169890645794467
>5k	0.0011323974830589126	2.9931297414523095
>10k+	4.901421941598278E-4	4.367548431962522
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	64851	0.42824234881066026	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	57933	0.3825594669881417	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	45950	0.3034299537069565	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	43262	0.28567979667617743	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36205	0.2390790309893441	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	33773	0.22301936510435347	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30421	0.2008844966641855	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	29703	0.19614319727873186	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	24940	0.16469081709361255	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	23061	0.1522828762227666	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	22017	0.1453888420188479	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	20439	0.13496854894051108	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	20016	0.13217527646133714	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	17995	0.11882964128306164	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTT	16988	0.11217993587755773	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	16545	0.10925459377761906	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	16315	0.10773579313882473	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	15872	0.10481045103888605	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG	15486	0.10226150735812686	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.8394968464415954E-4	6.603481038236268E-6	0.0	6.603481038236268E-6	0.0
2	2.8394968464415954E-4	6.603481038236268E-6	0.0	6.603481038236268E-6	0.0
3	2.8394968464415954E-4	6.603481038236268E-6	0.0	1.3206962076472537E-5	0.0
4	2.905531656823958E-4	6.603481038236268E-6	0.0	1.3206962076472537E-5	0.0
5	3.2357057087357716E-4	6.603481038236268E-6	0.0	1.9810443114708804E-5	0.0
6	3.43381013988286E-4	6.603481038236268E-6	0.0	3.301740519118134E-5	1.9810443114708804E-5
7	3.4998449502652223E-4	6.603481038236268E-6	0.0	3.301740519118134E-5	1.9810443114708804E-5
8	3.6319145710299474E-4	1.3206962076472537E-5	0.0	3.962088622941761E-5	1.9810443114708804E-5
9	3.6319145710299474E-4	1.3206962076472537E-5	0.0	5.282784830589015E-5	1.9810443114708804E-5
10	4.6884715371477506E-4	1.3206962076472537E-5	0.0	1.5188006387943418E-4	1.9810443114708804E-5
11	5.216750020206652E-4	1.3206962076472537E-5	0.0	1.5848354491767043E-4	1.9810443114708804E-5
12	5.41485445135374E-4	1.3206962076472537E-5	0.0	1.9150095010885178E-4	1.9810443114708804E-5
13	5.41485445135374E-4	1.3206962076472537E-5	0.0	1.9150095010885178E-4	1.9810443114708804E-5
14	5.480889261736103E-4	1.3206962076472537E-5	0.0	2.113113932235606E-4	2.6413924152945074E-5
15	5.612958882500828E-4	1.3206962076472537E-5	0.0	2.773462036059233E-4	7.263829142059895E-5
16	6.273306986324455E-4	1.3206962076472537E-5	0.0	3.1696708983534086E-4	9.244873453530776E-5
17	6.603481038236268E-4	1.3206962076472537E-5	0.0	3.6979493814123104E-4	9.244873453530776E-5
18	6.86762027976572E-4	1.3206962076472537E-5	0.0	3.962088622941761E-4	9.244873453530776E-5
19	6.999689900530445E-4	1.3206962076472537E-5	0.0	4.4243322956183E-4	9.244873453530776E-5
20	6.999689900530445E-4	1.3206962076472537E-5	0.0	5.216750020206652E-4	1.1225917765001656E-4
21	7.197794331677532E-4	1.3206962076472537E-5	0.0	6.273306986324455E-4	1.3206962076472536E-4
22	7.197794331677532E-4	1.3206962076472537E-5	0.0	7.594003193971708E-4	1.3206962076472536E-4
23	7.924177245883522E-4	3.301740519118134E-5	0.0	9.442977884677864E-4	1.3206962076472536E-4
24	8.12228167703061E-4	3.962088622941761E-5	0.0	0.0012150405110354734	1.3206962076472536E-4
25	8.254351297795335E-4	3.962088622941761E-5	0.0	0.0012876788024560723	1.3206962076472536E-4
26	8.584525349707149E-4	3.962088622941761E-5	0.0	0.0015188006387943417	1.650870259559067E-4
27	8.716594970471874E-4	3.962088622941761E-5	0.0	0.0018555781717443914	1.8489746907061552E-4
28	9.178838643148412E-4	5.282784830589015E-5	0.0	0.002806479441250414	1.9150095010885178E-4
29	9.442977884677864E-4	5.9431329344126414E-5	0.0	0.003915864255674107	1.9150095010885178E-4
30	9.509012695060226E-4	5.9431329344126414E-5	0.0	0.005989357301680295	1.9810443114708806E-4
31	9.575047505442589E-4	5.9431329344126414E-5	0.0	0.008399627880636534	1.9810443114708806E-4
32	9.905221557354401E-4	6.603481038236268E-5	0.0	0.010974985485548677	2.047079121853243E-4
33	9.905221557354401E-4	6.603481038236268E-5	0.0	0.013920138028602053	2.047079121853243E-4
34	0.0010433500040413304	6.603481038236268E-5	0.0	0.017367155130561387	2.311218363382694E-4
35	0.0010499534850795667	6.603481038236268E-5	0.0	0.021936764009020882	2.3772531737650566E-4
36	0.0010631604471560393	6.603481038236268E-5	0.0	0.02681013301523925	2.3772531737650566E-4
37	0.0010763674092325118	6.603481038236268E-5	0.0	0.03377020202954028	2.3772531737650566E-4
38	0.0010763674092325118	7.263829142059895E-5	0.0	0.04253962484831804	2.641392415294507E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCGT	4585	0.0	14.824074	9
CGTCGTA	4575	0.0	14.807124	10
CAATGCG	1155	0.0	14.667308	19
AATGCGA	1140	0.0	14.474556	20
TGCGACG	1120	0.0	14.342424	22
TACCGTC	4860	0.0	14.212875	7
CGACGAA	1235	0.0	14.072233	38
GTCGACG	1175	0.0	14.040709	36
GCATTCG	8430	0.0	14.0173025	22
GTACTAG	1635	0.0	13.998426	1
GGCGTTA	4865	0.0	13.973243	42
ATTCGTA	8405	0.0	13.902371	24
GACCGTC	2015	0.0	13.869039	22
ATACCGT	5160	0.0	13.856554	6
CCGTCGG	2035	0.0	13.841324	24
TCGACGA	1215	0.0	13.759463	37
CGACGAG	1275	0.0	13.634806	24
GACGGCC	8965	0.0	13.567783	11
GTCGTAG	5060	0.0	13.51898	11
CGAGCCG	6860	0.0	13.468953	15
>>END_MODULE