FastQCFastQC Report
Wed 25 May 2016
SRR2558174_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2558174_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4800014
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA489901.0206220231857657No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC341940.7123729222456435No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG206090.4293529143873331No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG183530.38235305147026655No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG179070.37306141190421527No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA175100.3647906026940755No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149470.3113949250981351No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC137950.287394995097931No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC128530.26777005233734735No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT119250.24843677539273845No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118850.24760344448995358No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102650.21385354292716646No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG86120.17941614336958184No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA82540.1719578317896573No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG69420.14462457817831365No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA68790.1433120820064275No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT68540.14279125019218694No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG61720.12858295829970495No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA57090.11893715309997013No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT48790.10164553686718414No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG48580.10120803814322207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGT13700.019.5881149
CGTCGTA14050.018.94181810
TACCGTC15000.018.772497
ATACCGT16050.018.5048056
GTACTAG7250.017.9063761
GTCGTAG16300.017.0021811
GCGCAGT8650.016.78222311
ACTGCGC8800.016.7492458
TGCGCAG10300.016.65597210
AATGCGA7300.016.57684120
TGCGACG7600.016.50321222
CGAACGT8600.016.3725514
CTGCGCA10300.016.0168619
CAATTCG5800.015.93081515
CAATGCG7500.015.84110319
TCGACGA4050.015.75106437
AATTCGT5650.015.57549416
AGATACC18950.015.5571634
GTCGACG4400.015.49801136
CGCATCG9000.015.39834613
Produced by FastQC (version 0.11.5)