##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2558174_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4800014
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.740784297712466	30.0	26.0	31.0	16.0	33.0
2	27.687008204559405	30.0	26.0	31.0	16.0	34.0
3	27.873647243528872	30.0	26.0	31.0	16.0	34.0
4	31.204049613188626	35.0	30.0	35.0	20.0	37.0
5	30.38388929698955	35.0	28.0	35.0	17.0	37.0
6	30.751374058492328	35.0	29.0	35.0	17.0	37.0
7	30.500010416636286	35.0	28.0	35.0	17.0	37.0
8	30.5544679661351	35.0	28.0	35.0	17.0	37.0
9	31.454714090417237	35.0	28.0	38.0	17.0	39.0
10	31.104158654537258	35.0	28.0	38.0	16.0	39.0
11	31.432747696152553	35.0	28.0	38.0	17.0	39.0
12	31.081572678746355	35.0	27.0	38.0	16.0	39.0
13	31.26413943792664	35.0	28.0	38.0	16.0	39.0
14	31.634311899923624	36.0	27.0	39.0	16.0	40.0
15	31.57560519615151	35.0	27.0	39.0	16.0	40.0
16	31.43827538836345	35.0	27.0	39.0	16.0	40.0
17	31.42288064159813	35.0	27.0	39.0	16.0	40.0
18	31.425958965953015	35.0	27.0	39.0	16.0	40.0
19	31.354799381835136	35.0	27.0	39.0	13.0	40.0
20	31.378294521640978	35.0	27.0	39.0	14.0	40.0
21	31.234930981451303	35.0	27.0	39.0	13.0	40.0
22	31.13769439005803	34.0	27.0	39.0	13.0	40.0
23	30.772567955010132	34.0	27.0	39.0	12.0	40.0
24	30.656108086351416	34.0	26.0	39.0	12.0	40.0
25	30.695787345620243	34.0	26.0	39.0	12.0	40.0
26	30.53230865576642	34.0	26.0	39.0	12.0	40.0
27	30.307087229328914	34.0	25.0	38.0	11.0	40.0
28	30.21371146000824	34.0	25.0	38.0	11.0	40.0
29	30.134550440894547	34.0	25.0	38.0	11.0	40.0
30	30.008626016507453	33.0	25.0	38.0	10.0	40.0
31	29.829928412708796	33.0	25.0	38.0	10.0	40.0
32	29.525257009667055	33.0	24.0	38.0	10.0	40.0
33	29.38046639030636	33.0	24.0	38.0	9.0	40.0
34	29.239977425065845	33.0	24.0	38.0	9.0	40.0
35	29.14761727778294	33.0	24.0	38.0	9.0	40.0
36	28.97572486246915	33.0	23.0	38.0	9.0	40.0
37	28.897485923999387	32.0	23.0	38.0	9.0	40.0
38	28.777330441119545	32.0	23.0	38.0	9.0	40.0
39	28.678656978917143	32.0	23.0	38.0	9.0	40.0
40	28.439387468453216	32.0	22.0	38.0	9.0	40.0
41	28.217763114857583	31.0	22.0	37.0	9.0	40.0
42	28.164221604353653	31.0	21.0	37.0	9.0	40.0
43	28.056445668700132	31.0	21.0	37.0	8.0	40.0
44	27.754079258935494	31.0	20.0	37.0	8.0	40.0
45	27.70233107653436	31.0	20.0	37.0	8.0	40.0
46	27.333525068885216	30.0	20.0	37.0	8.0	40.0
47	27.210985426292506	30.0	19.0	37.0	8.0	40.0
48	27.05504254779257	30.0	19.0	37.0	8.0	40.0
49	26.884757419457525	30.0	18.0	37.0	8.0	39.0
50	25.82098698045464	29.0	15.0	35.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	24.0
5	50.0
6	81.0
7	133.0
8	339.0
9	1650.0
10	5501.0
11	15298.0
12	31733.0
13	51029.0
14	67856.0
15	81082.0
16	90482.0
17	97990.0
18	103504.0
19	108348.0
20	113164.0
21	118407.0
22	124423.0
23	130829.0
24	138493.0
25	146106.0
26	155114.0
27	166527.0
28	177046.0
29	190145.0
30	205071.0
31	222033.0
32	241300.0
33	262610.0
34	286336.0
35	309497.0
36	331948.0
37	338343.0
38	319454.0
39	168064.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.38647422123556	29.69287878134287	13.145422502411611	18.775224495009958
2	17.758319899299956	29.059137107973793	32.43380518075216	20.748737811974088
3	20.64735398190264	31.541792435376692	27.63943451165628	20.171419071064392
4	15.673284124123215	23.363909046645688	31.699855347506624	29.26295148172447
5	14.50158617707883	42.0811476733465	30.471414953300236	12.945851196274438
6	30.129098630953955	38.50020285945351	17.341638866829385	14.029059642763148
7	27.524455725068204	33.89024756922168	21.272474919168534	17.312821786541583
8	30.286770729076608	32.47921221142311	19.116325970529196	18.11769108897109
9	29.24486921608836	14.334375976110136	19.049919118954005	37.37083568884751
10	18.570988681861685	30.7144764918313	28.428827564539805	22.28570726176721
11	35.158893255599224	25.019750197501978	20.63203965372987	19.189316893168932
12	24.470988801013522	27.736041899566484	27.217902542041784	20.575066757378213
13	30.31897815982681	21.691852442625454	24.591638743238427	23.39753065430931
14	26.06342515858373	21.738383982548235	25.377647533136162	26.820543325731872
15	26.741721777038112	27.45027774293809	23.67100702076478	22.13699345925902
16	24.687752879123884	27.9345020672022	24.9597268509606	22.418018202713323
17	24.849519901292556	28.020988249530486	25.71741868865944	21.412073160517522
18	25.323270720175294	24.82186953484064	28.70841113355258	21.14644861143148
19	25.36434649960025	26.88459702421795	28.218546014561984	19.53251046161982
20	26.130963630752653	25.02911527690926	27.903656303663222	20.936264788674873
21	25.71944754696851	25.81636437894877	26.897807024855386	21.56638104922733
22	25.401825335516943	26.517106711714145	25.974448970354814	22.106618982414105
23	23.33874294468735	27.170252305440496	27.223118961854738	22.26788578801742
24	23.680893068263263	28.18735243714596	26.256096069110008	21.875658425480772
25	25.50411742615121	24.909500819650606	26.017415022467972	23.568966731730214
26	25.01979646730999	25.957857280390495	26.550146743918734	22.472199508380783
27	25.013393914880343	25.804421544375174	26.521035062570576	22.661149478173908
28	25.520784491227282	25.005136887100598	28.489530127999142	20.984548493672982
29	25.581525201751944	25.44064157262966	27.91637654473003	21.061456680888373
30	25.830206561730485	25.930713283117473	27.39749887440389	20.84158128074815
31	26.673058541081407	25.36566946618541	27.30607082894248	20.655201163790704
32	24.013635085775707	26.257230640472663	28.65743753833227	21.07169673541936
33	24.292031443090504	26.23604487309979	27.604111429738836	21.867812254070873
34	23.758117847075074	26.229506147374476	27.74967132746202	22.262704678088426
35	24.72438579176019	27.25318378607341	27.599153504503438	20.423276917662957
36	25.013188796898362	25.907360057072275	26.996758847953384	22.08269229807598
37	26.866393704252197	26.068236992268655	26.448616200566537	20.616753102912607
38	26.344786921178493	24.876201032812688	26.853254964303687	21.92575708170513
39	26.779199475886006	24.953448208318644	26.647983436397414	21.619368879397936
40	25.897622356640255	26.71853545003532	26.265364251795575	21.11847794152885
41	24.232037184153356	27.12598071662709	26.387723012309785	22.25425908690977
42	27.659275671808075	26.72701311143333	25.99060840742768	19.62310280933092
43	26.809658568273946	24.477395635905886	26.37724102397641	22.335704771843755
44	25.60798685001296	26.485958537864146	27.250464075512397	20.655590536610493
45	27.991441371330843	24.80906336189752	25.894998236225735	21.304497030545903
46	25.0523402684189	25.97267054551395	27.99195893455883	20.983030251508325
47	26.71262373437323	25.50165145841498	26.781440578436516	21.004284228775276
48	26.95300152340651	26.65571742111511	25.70179114348839	20.689489911989988
49	25.94811660107504	25.563400808434455	28.35573116106247	20.13275142942804
50	27.518317660856535	26.291507860129265	24.03938409072584	22.150790388288364
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1329.0
1	1114.5
2	900.0
3	1107.0
4	1314.0
5	1873.5
6	2433.0
7	1935.5
8	1438.0
9	2152.5
10	2867.0
11	5441.0
12	8015.0
13	15875.5
14	23736.0
15	29373.0
16	35010.0
17	37373.0
18	39736.0
19	37860.5
20	35985.0
21	38977.5
22	41970.0
23	45800.0
24	49630.0
25	54922.0
26	60214.0
27	67554.5
28	74895.0
29	84663.5
30	94432.0
31	105258.0
32	116084.0
33	127834.5
34	139585.0
35	151851.0
36	164117.0
37	177858.5
38	191600.0
39	205956.5
40	220313.0
41	236663.0
42	253013.0
43	256896.0
44	260779.0
45	286512.0
46	312245.0
47	388448.5
48	464652.0
49	455491.0
50	446330.0
51	396809.0
52	347288.0
53	302584.5
54	257881.0
55	250885.5
56	243890.0
57	227007.5
58	210125.0
59	191946.5
60	173768.0
61	158081.0
62	142394.0
63	124555.0
64	106716.0
65	92027.0
66	77338.0
67	68247.0
68	59156.0
69	52069.0
70	44982.0
71	36854.5
72	28727.0
73	25713.0
74	22699.0
75	18924.5
76	15150.0
77	12283.5
78	9417.0
79	7617.5
80	5818.0
81	5059.0
82	4300.0
83	3548.0
84	2796.0
85	2402.0
86	2008.0
87	1698.5
88	1389.0
89	1020.0
90	651.0
91	521.0
92	391.0
93	321.5
94	252.0
95	197.5
96	143.0
97	106.0
98	69.0
99	56.5
100	44.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.028229084331837368
2	3.3333236111394673E-4
3	0.0041249879687850904
4	0.004895819053861094
5	0.015395788428950414
6	0.02343743164082438
7	0.01731244950535561
8	0.024229095998470004
9	0.020645773116495077
10	0.0022499934375191403
11	0.0012916628993165438
12	0.019145777491482316
13	0.00585414959206369
14	0.010395803012241214
15	0.005249984687544661
16	0.006041649045190285
17	0.007833310486177749
18	0.0179791142275835
19	0.016770784418545445
20	0.02056244002621659
21	0.02195826928838124
22	0.025520758897786548
23	0.023916596909925678
24	0.03406240065133143
25	0.03391656774334408
26	0.02454159508701433
27	0.024854094175558655
28	0.02891658232663488
29	0.0064166479514434745
30	0.00697914631082326
31	0.02527075962695109
32	0.031479074852698345
33	0.012770796085178086
34	0.008416642118127156
35	0.009604138654595591
36	0.010124970468836132
37	0.006874979947975151
38	0.010395803012241214
39	0.02204160237865973
40	0.01508328934040609
41	0.00697914631082326
42	0.012812462630317328
43	0.005312484505253527
44	0.025541592170356172
45	0.013479127352545223
46	0.013541627170254086
47	0.00660414740457007
48	0.018770778585229127
49	0.007645811033051154
50	0.008562475026114508
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4800014.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.58388716490614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.79413569875605	55.29871920942462
2	5.736569489911495	7.0656049636070515
3	1.8637762849603439	3.4433576530087726
4	0.8279544273250394	2.039546081202788
5	0.4394323434801512	1.3530975928745952
6	0.2751474352119076	1.0166789162282062
7	0.18304736042214095	0.7890937593049723
8	0.13712676599634852	0.6755839427526091
9	0.09423198780564665	0.5222854893912979
>10	0.5281838400046579	6.21568436637845
>50	0.0594719852232412	2.5475474128095468
>100	0.05067353047419836	6.325372852667896
>500	0.006182371772920324	2.6614153123279816
>1k	0.0034383234163654726	4.291120736979795
>5k	2.3142561456298503E-4	1.0062381990168026
>10k+	3.967296249651172E-4	4.748653512024725
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	48990	1.0206220231857657	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	34194	0.7123729222456435	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	20609	0.4293529143873331	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	18353	0.38235305147026655	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	17907	0.37306141190421527	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	17510	0.3647906026940755	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14947	0.3113949250981351	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	13795	0.287394995097931	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	12853	0.26777005233734735	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	11925	0.24843677539273845	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11885	0.24760344448995358	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10265	0.21385354292716646	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	8612	0.17941614336958184	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	8254	0.1719578317896573	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	6942	0.14462457817831365	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	6879	0.1433120820064275	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	6854	0.14279125019218694	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	6172	0.12858295829970495	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	5709	0.11893715309997013	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	4879	0.10164553686718414	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	4858	0.10120803814322207	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0019166610764051939	0.0	0.0	0.0	0.0
2	0.0019166610764051939	0.0	0.0	0.0	0.0
3	0.0019374943489748155	0.0	0.0	0.0	0.0
4	0.0019374943489748155	0.0	0.0	0.0	0.0
5	0.0020208274392533023	0.0	0.0	0.0	0.0
6	0.0020208274392533023	0.0	0.0	0.0	0.0
7	0.002041660711822924	0.0	0.0	0.0	0.0
8	0.002041660711822924	0.0	0.0	0.0	2.083327256962167E-5
9	0.002041660711822924	0.0	0.0	0.0	2.083327256962167E-5
10	0.002083327256962167	0.0	0.0	2.083327256962167E-5	4.166654513924334E-5
11	0.002104160529531789	0.0	0.0	4.166654513924334E-5	4.166654513924334E-5
12	0.002104160529531789	0.0	0.0	4.166654513924334E-5	4.166654513924334E-5
13	0.002104160529531789	0.0	0.0	4.166654513924334E-5	6.249981770886501E-5
14	0.002104160529531789	0.0	0.0	4.166654513924334E-5	6.249981770886501E-5
15	0.0021249938021014104	0.0	0.0	6.249981770886501E-5	1.2499963541773003E-4
16	0.0021249938021014104	0.0	0.0	6.249981770886501E-5	3.749989062531901E-4
17	0.0021458270746710323	0.0	0.0	6.249981770886501E-5	3.749989062531901E-4
18	0.0021666603472406537	0.0	0.0	8.333309027848668E-5	3.9583217882281174E-4
19	0.0021666603472406537	0.0	0.0	1.0416636284810836E-4	3.9583217882281174E-4
20	0.002208326892379897	0.0	0.0	1.2499963541773003E-4	3.9583217882281174E-4
21	0.002291659982658384	0.0	0.0	1.6666618055697336E-4	3.9583217882281174E-4
22	0.0023124932552280055	0.0	0.0	2.2916599826583838E-4	3.9583217882281174E-4
23	0.0023333265277976274	0.0	0.0	4.1666545139243345E-4	3.9583217882281174E-4
24	0.002354159800367249	0.0	0.0	4.999985416709201E-4	3.9583217882281174E-4
25	0.002354159800367249	0.0	0.0	5.208318142405418E-4	3.9583217882281174E-4
26	0.0023749930729368707	0.0	0.0	8.333309027848669E-4	3.9583217882281174E-4
27	0.002416659618076114	2.083327256962167E-5	0.0	9.166639930633535E-4	4.7916526910129847E-4
28	0.002479159435784979	2.083327256962167E-5	0.0	0.0012916628993165436	4.999985416709201E-4
29	0.0025208259809242225	2.083327256962167E-5	0.0	0.0019166610764051939	4.999985416709201E-4
30	0.002541659253493844	2.083327256962167E-5	0.0	0.0024999927083546006	4.999985416709201E-4
31	0.0025624925260634658	2.083327256962167E-5	0.0	0.003166657430582494	4.999985416709201E-4
32	0.002583325798633087	2.083327256962167E-5	0.0	0.004083321423645848	4.999985416709201E-4
33	0.002604159071202709	2.083327256962167E-5	0.0	0.005249984687544661	4.999985416709201E-4
34	0.0026458256163419524	2.083327256962167E-5	0.0	0.006270815043456123	5.416650868101634E-4
35	0.0026874921614811957	2.083327256962167E-5	0.0	0.007541644670203045	5.416650868101634E-4
36	0.0027083254340508176	2.083327256962167E-5	0.0	0.009645805199734835	5.416650868101634E-4
37	0.0027083254340508176	2.083327256962167E-5	0.0	0.011999965000102083	5.416650868101634E-4
38	0.0027083254340508176	2.083327256962167E-5	0.0	0.01545828824665928	5.833316319494068E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCGT	1370	0.0	19.588114	9
CGTCGTA	1405	0.0	18.941818	10
TACCGTC	1500	0.0	18.77249	7
ATACCGT	1605	0.0	18.504805	6
GTACTAG	725	0.0	17.906376	1
GTCGTAG	1630	0.0	17.00218	11
GCGCAGT	865	0.0	16.782223	11
ACTGCGC	880	0.0	16.749245	8
TGCGCAG	1030	0.0	16.655972	10
AATGCGA	730	0.0	16.576841	20
TGCGACG	760	0.0	16.503212	22
CGAACGT	860	0.0	16.372551	4
CTGCGCA	1030	0.0	16.016861	9
CAATTCG	580	0.0	15.930815	15
CAATGCG	750	0.0	15.841103	19
TCGACGA	405	0.0	15.751064	37
AATTCGT	565	0.0	15.575494	16
AGATACC	1895	0.0	15.557163	4
GTCGACG	440	0.0	15.498011	36
CGCATCG	900	0.0	15.398346	13
>>END_MODULE