FastQCFastQC Report
Wed 25 May 2016
SRR2558173_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2558173_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14014595
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT584260.4168939594758179No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA419430.29928085684959144No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT375120.26766381761299557No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC305830.2182225030405802No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291950.20831854220546508No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT291810.20821864634689766No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA235840.1682817091753276No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA173540.12382805211281524No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG170730.12182299952299729No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT169820.1211736764423089No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG149070.10636768311891995No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA148740.10613221430943955No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG148040.10563273501660232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCGA21400.019.0201720
TGCGACG20900.018.52987322
CAATGCG21650.018.08845319
CGACGAG22150.017.98171224
CGACGAA26000.017.59910638
GTACTAG26650.017.4239081
CATTGCG21350.017.41431629
TCGACGA25450.017.2864437
TAGGTCG21200.017.02049421
GCTCGTC21750.016.89121439
GTCGACG26750.016.44635236
CGTCATT22800.016.40718526
AGTCGAC27300.016.03438235
AGGTCGT22550.015.80791222
CAATTCG29200.015.5205915
ACAACGA29200.015.37017844
CGAACAA29600.015.30970341
GTCGTCA23000.015.30799524
TCAGCGC24300.015.30072544
GACGAAC29550.015.261600539
Produced by FastQC (version 0.11.5)