##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2558173_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14014595
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.642345426321633	31.0	30.0	34.0	26.0	34.0
2	30.77027877009646	31.0	30.0	34.0	27.0	34.0
3	30.99259536219206	31.0	31.0	34.0	27.0	34.0
4	34.630681015041816	37.0	35.0	37.0	31.0	37.0
5	34.24013166274159	35.0	35.0	37.0	30.0	37.0
6	34.274343354196105	37.0	35.0	37.0	30.0	37.0
7	34.133979540614625	36.0	35.0	37.0	29.0	37.0
8	34.120104933464006	37.0	35.0	37.0	29.0	37.0
9	35.53685019081893	38.0	35.0	39.0	29.0	39.0
10	35.45041958044453	38.0	35.0	39.0	28.0	39.0
11	35.52906045447621	38.0	35.0	39.0	29.0	39.0
12	35.38948803015713	38.0	35.0	39.0	28.0	39.0
13	35.418049897267814	38.0	35.0	39.0	28.0	39.0
14	36.33794997286757	39.0	36.0	41.0	28.0	41.0
15	36.319055384761384	39.0	36.0	41.0	28.0	41.0
16	36.30262537019443	39.0	36.0	41.0	27.0	41.0
17	36.25633997985671	39.0	36.0	41.0	27.0	41.0
18	36.24506780253015	39.0	36.0	41.0	27.0	41.0
19	36.2870183547937	39.0	36.0	41.0	27.0	41.0
20	36.254398646553824	39.0	36.0	41.0	27.0	41.0
21	36.18698913525507	39.0	35.0	41.0	27.0	41.0
22	36.13332272534454	39.0	35.0	40.0	27.0	41.0
23	35.88376538886782	39.0	35.0	40.0	27.0	41.0
24	35.851607199494524	39.0	34.0	40.0	27.0	41.0
25	35.84214299449966	39.0	35.0	40.0	27.0	41.0
26	35.77966826725995	39.0	34.0	40.0	27.0	41.0
27	35.576620301906694	39.0	34.0	40.0	26.0	41.0
28	35.56076861300666	39.0	34.0	40.0	26.0	41.0
29	35.52763893640879	38.0	34.0	40.0	26.0	41.0
30	35.42237360408917	38.0	34.0	40.0	25.0	41.0
31	35.32771464319875	38.0	34.0	40.0	25.0	41.0
32	35.20380745929511	38.0	34.0	40.0	25.0	41.0
33	35.063254557124196	38.0	34.0	40.0	25.0	41.0
34	34.959780214840315	38.0	33.0	40.0	25.0	41.0
35	34.87449990527732	38.0	33.0	40.0	24.0	41.0
36	34.70548167820761	38.0	33.0	40.0	24.0	41.0
37	34.67026218024852	38.0	33.0	40.0	24.0	41.0
38	34.58796847143995	38.0	33.0	40.0	24.0	41.0
39	34.529241908167876	38.0	33.0	40.0	23.0	41.0
40	34.394919225279075	38.0	33.0	40.0	23.0	41.0
41	34.26218281727014	38.0	32.0	40.0	23.0	41.0
42	34.182788443048125	38.0	32.0	40.0	22.0	41.0
43	34.083501806509574	38.0	32.0	40.0	22.0	41.0
44	33.908008258533336	37.0	32.0	40.0	21.0	41.0
45	33.80588436554891	37.0	31.0	40.0	21.0	41.0
46	33.590123724588544	37.0	31.0	40.0	20.0	41.0
47	33.44331755573386	37.0	31.0	40.0	20.0	41.0
48	33.30855854200567	37.0	31.0	40.0	20.0	41.0
49	33.212930591287154	37.0	31.0	40.0	19.0	41.0
50	31.940377228168206	35.0	28.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	30.0
5	82.0
6	190.0
7	306.0
8	633.0
9	1713.0
10	4493.0
11	11198.0
12	24415.0
13	41776.0
14	59633.0
15	72143.0
16	81591.0
17	87078.0
18	92162.0
19	96130.0
20	101034.0
21	106075.0
22	113506.0
23	122802.0
24	135771.0
25	151394.0
26	172576.0
27	196428.0
28	226213.0
29	263937.0
30	317024.0
31	387570.0
32	486234.0
33	622827.0
34	816088.0
35	1077340.0
36	1434262.0
37	1903378.0
38	2366323.0
39	2440235.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.08700256799431	20.108043645766692	13.578967716826481	24.225986069412514
2	15.400054843037669	22.280430019078043	37.12177230777662	25.19774283010767
3	17.071454989448544	27.808509999105873	29.601169655158238	25.51886535628734
4	12.506163756469716	18.34033498009401	36.65647164101141	32.497029622424876
5	13.661038233824351	38.33805767324962	33.53658057895919	14.464323513966837
6	30.799273561569684	37.65107486849649	17.816106639471382	13.733544930462449
7	26.981855854427184	32.43627595853072	22.900616752073116	17.681251434968974
8	27.40959609445221	32.67755041619353	20.56046963301409	19.352383856340158
9	27.327516323850233	14.45802162200896	20.272200015216512	37.9422620389243
10	17.066893789880297	27.68556526464797	30.95942622465361	24.288114720818125
11	33.68511749011907	23.72053827606949	23.12609842611548	19.468245807695958
12	23.42327534617261	25.718436405692923	29.372202062101422	21.486086186033052
13	28.401968767547892	21.329399775372735	25.846328777158668	24.42230267992071
14	23.69533938709807	20.728661164394637	26.78033020768467	28.79566924082262
15	24.586304324339523	27.785098463902326	23.946937973371796	23.681659238386356
16	23.62674963827655	27.295234841225597	25.414772417505787	23.663243102992066
17	22.472991299725994	27.28052853044182	26.8168126893435	23.429667480488682
18	23.27081828909405	25.848188203736743	28.720438292842417	22.160555214326795
19	23.70263605836655	26.19034432553376	28.460259287939504	21.646760328160184
20	24.443061551291127	25.195690721584736	27.77807595866185	22.58317176846229
21	24.981614419548098	25.629377816541027	27.135625059774547	22.253382704136328
22	24.445462117222057	25.406656117669872	27.188710436890208	22.959171328217863
23	22.847748341506428	25.763724995192927	27.725310382720064	23.66321628058058
24	23.249520826137072	26.58555313734942	27.35650713858591	22.8084188979276
25	23.53678017185319	25.132511867069972	27.328567612639194	24.00214034843765
26	23.54798178061018	25.90116260811125	27.474382194926	23.07647341635257
27	23.889064946818607	25.63773416299443	27.23251209446602	23.240688795720935
28	23.15946339584521	25.470923424780313	28.28878335252537	23.080829826849108
29	23.195286100860905	25.30867117987415	28.60440586841138	22.891636850853573
30	23.461157096743232	25.83457945892638	28.127343509995946	22.576919934334445
31	23.915415948269096	25.26891551872944	27.597936896356895	23.217731636644572
32	23.183098418628184	25.70180681912436	27.764131464675142	23.350963297572317
33	23.16121332569528	25.822861162591686	27.61146156122726	23.404463950485777
34	23.107561476117606	25.886821202242132	27.92439551493447	23.08122180670579
35	24.010957074088704	25.85746740584456	27.86780576277585	22.263769757290888
36	23.410012846168048	25.890841183868478	27.049622489935764	23.64952348002771
37	23.973189851106266	26.093146892070774	27.317807661979472	22.615855594843488
38	23.488340427572606	25.506448471530767	27.533826625955253	23.47138447494137
39	23.875286789636924	25.297492167596136	27.474727360257745	23.352493682509195
40	23.875063811298546	25.75462190775247	27.49357212455566	22.87674215639333
41	22.74838794390416	26.127204713283795	27.65172166734698	23.472685675465062
42	24.369406543767518	26.180900627988613	27.177984915598774	22.2717079126451
43	24.286427364699122	25.012311188892276	27.218841595798388	23.48241985061021
44	23.57456195136408	26.020376625966612	27.210100461285652	23.194960961383654
45	24.3022901639363	25.71569407159667	26.72773551641019	23.254280248056848
46	23.2648103854464	25.890517145652193	27.65461659605844	23.190055872842965
47	23.789117768140784	26.011190609073896	27.2131795400631	22.986512082722218
48	24.70445945672998	26.37259744728339	26.140896944449622	22.78204615153701
49	23.755438621705135	26.422522792598045	26.9600571052157	22.86198148048112
50	24.309100074587732	26.42430315560634	25.923661895202095	23.34293487460383
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4801.0
1	3944.0
2	3087.0
3	5736.0
4	8385.0
5	9170.5
6	9956.0
7	7772.5
8	5589.0
9	8242.0
10	10895.0
11	17479.0
12	24063.0
13	47925.5
14	71788.0
15	90752.5
16	109717.0
17	104153.0
18	98589.0
19	90314.0
20	82039.0
21	89802.5
22	97566.0
23	111588.5
24	125611.0
25	148550.5
26	171490.0
27	195681.5
28	219873.0
29	252993.5
30	286114.0
31	323009.5
32	359905.0
33	398094.5
34	436284.0
35	482805.5
36	529327.0
37	579892.5
38	630458.0
39	675314.0
40	720170.0
41	765283.0
42	810396.0
43	822876.0
44	835356.0
45	896480.5
46	957605.0
47	1020882.0
48	1084159.0
49	1081876.0
50	1079593.0
51	975247.5
52	870902.0
53	822399.0
54	773896.0
55	764814.5
56	755733.0
57	718050.0
58	680367.0
59	632770.5
60	585174.0
61	518685.0
62	452196.0
63	392444.0
64	332692.0
65	279891.5
66	227091.0
67	198991.5
68	170892.0
69	149678.5
70	128465.0
71	103723.5
72	78982.0
73	71104.0
74	63226.0
75	54674.0
76	46122.0
77	35813.5
78	25505.0
79	20370.5
80	15236.0
81	13390.0
82	11544.0
83	9897.5
84	8251.0
85	7155.5
86	6060.0
87	5055.0
88	4050.0
89	2977.0
90	1904.0
91	1595.5
92	1287.0
93	1092.5
94	898.0
95	746.0
96	594.0
97	514.0
98	434.0
99	356.0
100	278.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04279110455921131
2	3.853125973315675E-4
3	0.006364793274439967
4	0.0087408876246513
5	0.019700890393193667
6	0.030846414041932713
7	0.02117792201629801
8	0.029726153342283526
9	0.024981100060329966
10	0.0033465112620093555
11	0.001605469155548198
12	0.022547922362365806
13	0.008262814587221393
14	0.012251513511450028
15	0.007370887278583505
16	0.008041616614679198
17	0.012151617652882583
18	0.022412349411452846
19	0.02244089108532926
20	0.026479537938841616
21	0.025794537765807715
22	0.03151000795955931
23	0.02846318427325228
24	0.03987271840534814
25	0.04039360395359266
26	0.028734330175078194
27	0.028934121892213085
28	0.03789620748940658
29	0.0075492727403110825
30	0.0082770854241596
31	0.030304122238280877
32	0.03648339463252417
33	0.016397191641998928
34	0.010781617306814787
35	0.012344273951548369
36	0.01324333667865536
37	0.008883595994033364
38	0.01296505535836034
39	0.02775677784481107
40	0.0183951088133478
41	0.010239325503162953
42	0.01597620195232185
43	0.006564584991574855
44	0.03297276874572544
45	0.016575577103726508
46	0.014720368301759702
47	0.008833648064749641
48	0.024288964468826963
49	0.008305627098036011
50	0.011181200741084562
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1.4014595E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.84441133891896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.17119226387291	24.087471064598013
2	11.557521793217077	7.823150432563178
3	5.719021242076382	5.806707221185448
4	3.2721197543211566	4.429718676617906
5	2.0491710742382576	3.4676494370167004
6	1.3371201446558825	2.7152426511173102
7	0.9260645721291215	2.193947721387658
8	0.6654061795830106	1.801622435941279
9	0.4947059340380612	1.5068728011049426
>10	2.4761919409884854	14.636500511194736
>50	0.16832449210174824	3.971863864109953
>100	0.1299722023613451	9.04604416450817
>500	0.018150751585944383	4.253351171594476
>1k	0.013619413224192082	8.965450756297669
>5k	0.0010428247108763464	2.4391466623548204
>10k+	3.754168959154847E-4	2.8552604284077296
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58426	0.4168939594758179	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	41943	0.29928085684959144	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37512	0.26766381761299557	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	30583	0.2182225030405802	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29195	0.20831854220546508	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	29181	0.20821864634689766	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	23584	0.1682817091753276	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	17354	0.12382805211281524	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	17073	0.12182299952299729	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	16982	0.1211736764423089	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	14907	0.10636768311891995	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	14874	0.10613221430943955	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	14804	0.10563273501660232	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.281251081461862E-4	0.0	0.0	0.0	0.0
2	4.281251081461862E-4	7.135418469103103E-6	0.0	0.0	0.0
3	4.495313635534955E-4	7.135418469103103E-6	0.0	0.0	0.0
4	4.495313635534955E-4	7.135418469103103E-6	0.0	0.0	0.0
5	4.495313635534955E-4	7.135418469103103E-6	0.0	7.135418469103103E-6	0.0
6	4.495313635534955E-4	7.135418469103103E-6	0.0	1.4270836938206206E-5	2.140625540730931E-5
7	4.495313635534955E-4	7.135418469103103E-6	0.0	1.4270836938206206E-5	3.5677092345515516E-5
8	4.495313635534955E-4	7.135418469103103E-6	0.0	1.4270836938206206E-5	4.281251081461862E-5
9	4.780730374299079E-4	7.135418469103103E-6	0.0	1.4270836938206206E-5	4.281251081461862E-5
10	4.994792928372172E-4	7.135418469103103E-6	0.0	3.5677092345515516E-5	6.421876622192792E-5
11	4.994792928372172E-4	7.135418469103103E-6	0.0	4.994792928372172E-5	6.421876622192792E-5
12	4.994792928372172E-4	7.135418469103103E-6	0.0	5.708334775282482E-5	6.421876622192792E-5
13	5.066147113063203E-4	7.135418469103103E-6	0.0	5.708334775282482E-5	6.421876622192792E-5
14	5.137501297754234E-4	1.4270836938206206E-5	0.0	7.848960316013413E-5	6.421876622192792E-5
15	5.137501297754234E-4	1.4270836938206206E-5	0.0	1.0703127703654655E-4	7.848960316013413E-5
16	5.208855482445265E-4	1.4270836938206206E-5	0.0	1.4270836938206206E-4	9.276044009834034E-5
17	5.208855482445265E-4	1.4270836938206206E-5	0.0	1.5697920632026826E-4	9.276044009834034E-5
18	5.208855482445265E-4	1.4270836938206206E-5	0.0	1.6411462478937137E-4	1.0703127703654655E-4
19	5.280209667136296E-4	1.4270836938206206E-5	0.0	2.0692713560398998E-4	1.0703127703654655E-4
20	5.280209667136296E-4	1.4270836938206206E-5	0.0	2.568750648877117E-4	1.2843753244385584E-4
21	5.422918036518358E-4	1.4270836938206206E-5	0.0	3.9958343426977375E-4	1.3557295091295895E-4
22	5.636980590591451E-4	1.4270836938206206E-5	0.0	5.422918036518358E-4	1.3557295091295895E-4
23	5.708334775282483E-4	1.4270836938206206E-5	7.135418469103103E-6	7.706251946631352E-4	1.3557295091295895E-4
24	5.708334775282483E-4	1.4270836938206206E-5	7.135418469103103E-6	0.0010845836073036716	1.3557295091295895E-4
25	5.708334775282483E-4	1.4270836938206206E-5	7.135418469103103E-6	0.001255833650562146	1.3557295091295895E-4
26	6.065105698737637E-4	1.4270836938206206E-5	7.135418469103103E-6	0.0014841670415734454	1.3557295091295895E-4
27	6.27916825281073E-4	1.4270836938206206E-5	7.135418469103103E-6	0.0017481775249302601	1.6411462478937137E-4
28	6.350522437501762E-4	1.4270836938206206E-5	7.135418469103103E-6	0.002697188181320973	1.6411462478937137E-4
29	6.350522437501762E-4	1.4270836938206206E-5	7.135418469103103E-6	0.00411713645667249	1.6411462478937137E-4
30	6.350522437501762E-4	1.4270836938206206E-5	7.135418469103103E-6	0.006792918382586154	1.6411462478937137E-4
31	6.421876622192792E-4	1.4270836938206206E-5	7.135418469103103E-6	0.009761252465733044	1.7125004325847448E-4
32	6.493230806883823E-4	1.4270836938206206E-5	7.135418469103103E-6	0.012715315711941729	1.7125004325847448E-4
33	6.493230806883823E-4	1.4270836938206206E-5	7.135418469103103E-6	0.015997608207729157	1.7125004325847448E-4
34	6.707293360956917E-4	1.4270836938206206E-5	7.135418469103103E-6	0.019922088365735863	1.7125004325847448E-4
35	6.778647545647948E-4	1.4270836938206206E-5	7.135418469103103E-6	0.0246100582999366	1.7125004325847448E-4
36	6.778647545647948E-4	1.4270836938206206E-5	7.135418469103103E-6	0.030247038890528054	1.7125004325847448E-4
37	6.992710099721041E-4	1.4270836938206206E-5	7.135418469103103E-6	0.038210165902047115	1.7125004325847448E-4
38	7.135418469103103E-4	1.4270836938206206E-5	7.135418469103103E-6	0.04746480365647384	1.7838546172757757E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATGCGA	2140	0.0	19.02017	20
TGCGACG	2090	0.0	18.529873	22
CAATGCG	2165	0.0	18.088453	19
CGACGAG	2215	0.0	17.981712	24
CGACGAA	2600	0.0	17.599106	38
GTACTAG	2665	0.0	17.423908	1
CATTGCG	2135	0.0	17.414316	29
TCGACGA	2545	0.0	17.28644	37
TAGGTCG	2120	0.0	17.020494	21
GCTCGTC	2175	0.0	16.891214	39
GTCGACG	2675	0.0	16.446352	36
CGTCATT	2280	0.0	16.407185	26
AGTCGAC	2730	0.0	16.034382	35
AGGTCGT	2255	0.0	15.807912	22
CAATTCG	2920	0.0	15.52059	15
ACAACGA	2920	0.0	15.370178	44
CGAACAA	2960	0.0	15.309703	41
GTCGTCA	2300	0.0	15.307995	24
TCAGCGC	2430	0.0	15.300725	44
GACGAAC	2955	0.0	15.2616005	39
>>END_MODULE