FastQCFastQC Report
Wed 25 May 2016
SRR2558172_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2558172_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11908692
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA729250.6123678402296406No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC600500.5042535317900572No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT531970.4467073294027589No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA462110.38804429571274496No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG326350.2740435305573442No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300950.25271457184382634No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG283460.2380278203517229No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG262030.22003256109067226No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA261710.2197638497997933No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC219730.1845122873276091No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190920.16031987392066233No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC183620.15418989759748594No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT175330.14722859571815278No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167360.14053600512969855No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG163740.13749620865163026No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC149890.12586604809327506No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC139690.11730087569650806No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA138100.11596571646995321No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC136660.11475651566099786No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG127770.10729138011126663No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA127550.10710664109878734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG13850.020.65478124
TGCGACG14950.018.69282522
AATGCGA15600.018.33442520
CAATGCG15800.017.54527519
GACGAGT15800.017.40851225
ATCGTCA16500.016.79549410
TCGACGA15150.016.5524537
GTACTAG19700.016.0862981
GTCGACG15200.016.06384336
CGTCGTA27700.016.0390210
CCGTCGG27650.015.99660924
CGACGAA16100.015.98684938
GACCGTC28650.015.97537622
CCGTCGT28400.015.8775579
CATTGCG16400.015.829144529
GTGATCG19800.015.776338
CAATTCG17050.015.61245315
GTCGGCT28750.015.46110426
CGAACAA17800.015.44886541
TGATCGC20150.015.39199
Produced by FastQC (version 0.11.5)