##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2558172_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11908692
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.29557469451725	31.0	30.0	33.0	26.0	34.0
2	30.399009899659845	31.0	30.0	34.0	26.0	34.0
3	30.63248885771838	31.0	30.0	34.0	26.0	34.0
4	34.296098597562185	35.0	35.0	37.0	30.0	37.0
5	33.863055657162015	35.0	35.0	37.0	28.0	37.0
6	33.91787964622815	35.0	35.0	37.0	28.0	37.0
7	33.7650541302101	35.0	35.0	37.0	28.0	37.0
8	33.756154076367075	35.0	35.0	37.0	28.0	37.0
9	35.09709479428975	38.0	35.0	39.0	28.0	39.0
10	34.993991279646835	37.0	34.0	39.0	27.0	39.0
11	35.09170360607193	38.0	35.0	39.0	27.0	39.0
12	34.92892410014467	37.0	34.0	39.0	27.0	39.0
13	34.96477245359944	37.0	34.0	39.0	27.0	39.0
14	35.80323548547565	38.0	34.0	40.0	27.0	41.0
15	35.77694057416213	38.0	34.0	40.0	27.0	41.0
16	35.73346955316335	38.0	34.0	40.0	27.0	41.0
17	35.71258044124409	38.0	34.0	40.0	27.0	41.0
18	35.69768871342042	38.0	34.0	40.0	27.0	41.0
19	35.73479337613232	39.0	34.0	40.0	27.0	41.0
20	35.71074010479069	39.0	34.0	40.0	27.0	41.0
21	35.62514397047131	39.0	34.0	40.0	27.0	41.0
22	35.57320224588897	39.0	34.0	40.0	26.0	41.0
23	35.314842049823774	38.0	34.0	40.0	25.0	41.0
24	35.27548575443886	38.0	34.0	40.0	25.0	41.0
25	35.26747060046561	38.0	34.0	40.0	25.0	41.0
26	35.191007123200436	38.0	34.0	40.0	25.0	41.0
27	34.98865752846744	38.0	33.0	40.0	24.0	41.0
28	34.96703357513991	38.0	33.0	40.0	24.0	41.0
29	34.930057557958506	38.0	33.0	40.0	24.0	41.0
30	34.82418975988295	38.0	33.0	40.0	24.0	41.0
31	34.709613952565064	38.0	33.0	40.0	24.0	41.0
32	34.5490037025057	38.0	33.0	40.0	24.0	41.0
33	34.43102886530276	38.0	33.0	40.0	24.0	41.0
34	34.320117104380564	38.0	32.0	40.0	23.0	41.0
35	34.24033294336607	38.0	32.0	40.0	23.0	41.0
36	34.068358640898595	38.0	32.0	40.0	22.0	41.0
37	34.01712127578747	38.0	32.0	40.0	22.0	41.0
38	33.92918584173644	37.0	32.0	40.0	22.0	41.0
39	33.86149318497783	37.0	31.0	40.0	21.0	41.0
40	33.70998015567117	37.0	31.0	40.0	21.0	41.0
41	33.571875819779365	37.0	31.0	40.0	20.0	41.0
42	33.50256678063384	37.0	31.0	40.0	20.0	41.0
43	33.37092234814705	37.0	31.0	40.0	19.0	41.0
44	33.179756013506776	37.0	30.0	40.0	18.0	41.0
45	33.07887843601967	37.0	30.0	40.0	18.0	41.0
46	32.8360007127567	37.0	30.0	40.0	17.0	41.0
47	32.708740892786544	36.0	30.0	40.0	16.0	41.0
48	32.57616008542332	36.0	30.0	40.0	15.0	41.0
49	32.4595500496612	36.0	30.0	39.0	15.0	41.0
50	31.245902908564602	35.0	27.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	35.0
5	71.0
6	165.0
7	289.0
8	584.0
9	1771.0
10	4756.0
11	11609.0
12	24077.0
13	40579.0
14	56852.0
15	70280.0
16	78823.0
17	84914.0
18	90653.0
19	94110.0
20	99958.0
21	105040.0
22	112762.0
23	122755.0
24	135741.0
25	153153.0
26	173028.0
27	196507.0
28	226289.0
29	264411.0
30	314955.0
31	381919.0
32	473961.0
33	596639.0
34	759839.0
35	966415.0
36	1234777.0
37	1549372.0
38	1746814.0
39	1734784.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.34437023950045	21.286504776324307	14.048928331319555	23.320196652855692
2	14.655230615444765	23.63443868056983	37.508170527387804	24.202160176597594
3	17.14901383979164	29.282548661389285	30.30224499299004	23.266192505829036
4	12.33993620290379	19.328845612505198	36.699277915764455	31.631940268826558
5	12.804112990322658	39.715134736396706	33.8204745973849	13.660277675895738
6	29.462902453570315	38.16041864411051	18.59294756028929	13.783731342029887
7	25.660048723711228	33.49756826585683	23.47906380461197	17.36331920581997
8	27.39515679379877	33.074094849215314	20.92069602210923	18.610052334876688
9	27.296609177961944	15.159534326289254	20.346729651510724	37.19712684423808
10	16.565598750252867	28.82362420453785	31.72503883626326	22.885738208946023
11	32.76048634709971	24.479373276235897	23.053370474211263	19.706769902453132
12	22.766046299187785	26.971521979675046	29.521607424318958	20.740824296818214
13	28.13868377140717	21.98186736268046	26.39240128957382	23.487047576338554
14	23.469112841038136	21.90462849260022	26.880397028834373	27.74586163752727
15	24.996468716015066	27.71166079518973	25.069655731303296	22.222214757491905
16	23.106213485099275	28.2442699371253	26.13960081660692	22.50991576116851
17	22.352563063026366	27.93651316201092	27.33535823440406	22.375565540558643
18	23.03947996915521	26.214954515191124	29.60679781385333	21.138767701800333
19	23.27578423265789	27.525769429278274	29.156358544993665	20.04208779307017
20	24.409721898495476	25.284552661151455	29.20034299776316	21.105382442589914
21	24.214605167704857	26.771647194268823	27.22565177538696	21.788095862639363
22	23.46319189260451	26.838431422684145	27.067283288538736	22.63109339617261
23	21.934421349482054	27.10420317340003	27.741572729812457	23.21980274730545
24	22.51376834238902	28.38460969815492	27.262645201829283	21.83897675762677
25	23.517636232053896	25.604615909970097	27.054215604863725	23.82353225311228
26	22.801270645938565	26.38718817423688	27.757278671389113	23.054262508435443
27	23.649786071915486	26.344607119814935	27.31505953422417	22.690547274045418
28	22.466889855294774	26.182566371287415	28.676717351312753	22.673826422105066
29	22.84402928558484	26.156141862191944	29.145945720793314	21.853883131429903
30	23.021658496078857	26.817303551501574	28.69974114341649	21.461296809003077
31	23.8190591010762	26.011102592853323	27.59622371735519	22.573614588715284
32	21.967669726459928	26.753092189950227	28.474675307874964	22.80456277571488
33	22.35023151777786	26.745730885747303	27.970814474108668	22.933223122366172
34	21.709428095302922	27.109069209865005	28.399701329112325	22.781801365719748
35	23.227313535244612	27.113698339073554	28.111018450165908	21.547969675515926
36	22.899485330465343	27.042507057578792	27.28464915514815	22.773358456807717
37	23.873835288711344	26.80487000313747	27.621788708993666	21.699505999157516
38	23.471615720524017	26.360257473618226	27.4890057045033	22.679121101354454
39	23.632632401749998	25.849509894422646	27.788874521918384	22.728983181908973
40	23.500818376791962	26.62533964652573	27.35513454168212	22.518707435000188
41	21.92844941025641	27.30663114384651	27.836298966454905	22.928620479442177
42	24.790448571872258	26.95515473878961	27.2393970374602	21.014999651877933
43	23.953272296331328	25.33184503015124	27.08177415323924	23.63310852027819
44	23.119329571471674	26.57641357092601	27.72379649658993	22.580460361012385
45	24.86332223449827	25.97885804199416	26.888744151904966	22.269075571602603
46	22.62816432533796	26.73361864265784	28.532659748684015	22.105557283320188
47	23.813343280995017	26.527521453424583	27.44853395160223	22.210601313978167
48	24.414171788962584	27.42540312725048	26.60539061905489	21.555034464732046
49	23.262883390734117	26.956682905052176	28.412511033740678	21.367922670473025
50	24.087130019317325	27.33758355906044	25.66265679474843	22.912629626873805
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1287.0
1	1102.5
2	918.0
3	1815.0
4	2712.0
5	3451.0
6	4190.0
7	4048.5
8	3907.0
9	5298.5
10	6690.0
11	10438.5
12	14187.0
13	29454.5
14	44722.0
15	61416.0
16	78110.0
17	81224.0
18	84338.0
19	88003.0
20	91668.0
21	108594.0
22	125520.0
23	141327.0
24	157134.0
25	174836.5
26	192539.0
27	211344.5
28	230150.0
29	254602.5
30	279055.0
31	334623.0
32	390191.0
33	416255.0
34	442319.0
35	471544.0
36	500769.0
37	526978.5
38	553188.0
39	588991.0
40	624794.0
41	656101.0
42	687408.0
43	681033.0
44	674658.0
45	741043.5
46	807429.0
47	928099.5
48	1048770.0
49	1043021.0
50	1037272.0
51	918351.5
52	799431.0
53	679527.5
54	559624.0
55	544654.5
56	529685.0
57	499918.5
58	470152.0
59	432876.0
60	395600.0
61	346709.0
62	297818.0
63	259444.5
64	221071.0
65	187573.5
66	154076.0
67	137385.0
68	120694.0
69	103538.5
70	86383.0
71	70408.5
72	54434.0
73	48907.5
74	43381.0
75	36251.0
76	29121.0
77	23683.5
78	18246.0
79	15480.0
80	12714.0
81	11263.0
82	9812.0
83	8642.0
84	7472.0
85	6582.5
86	5693.0
87	4720.5
88	3748.0
89	2885.5
90	2023.0
91	1688.0
92	1353.0
93	1126.0
94	899.0
95	765.0
96	631.0
97	523.0
98	415.0
99	353.0
100	291.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04091969126416234
2	3.023002022388353E-4
3	0.005349034134059391
4	0.007893394169569587
5	0.018650243032568143
6	0.03016284240116379
7	0.019935018892083194
8	0.028869669313808768
9	0.02316795161047074
10	0.0030481937059082557
11	0.0013435564543948235
12	0.022991609825831417
13	0.007406354954851465
14	0.012142391456593217
15	0.007070465841252759
16	0.007406354954851465
17	0.01074005440731862
18	0.022076311991274945
19	0.022059517535595007
20	0.025821475607900518
21	0.02405805776150731
22	0.030322389730123172
23	0.028164302175251486
24	0.03839212568433208
25	0.039021917772329655
26	0.02994451447732463
27	0.029583433680206023
28	0.03829975617809244
29	0.007171232575332371
30	0.007893394169569587
31	0.029768172692685307
32	0.03513400128242464
33	0.01537532417498076
34	0.01067287658459888
35	0.012444691658832052
36	0.013721070290507136
37	0.008565172396767
38	0.013502742366667977
39	0.0276940574162133
40	0.018440312336568954
41	0.010320193015320238
42	0.016366197060096944
43	0.006079592956136576
44	0.03315225551219227
45	0.017105153110014096
46	0.01552647427610018
47	0.008699528042206483
48	0.024335166280226243
49	0.007549107828130914
50	0.010462945888599688
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1.1908692E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.1586510521264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.9674335768854	27.485300535048225
2	11.89009762627762	8.83639977341133
3	5.460766090430608	6.0874410489478645
4	2.8224808498438256	4.195183240026235
5	1.6571381230759168	3.0788508630276836
6	1.0200318590183657	2.2741804746789147
7	0.6755707304456811	1.7572307923562842
8	0.4609186329718437	1.3701691716819089
9	0.3328625383915416	1.113185062117469
>10	1.4711999640752962	9.606503400979625
>50	0.10807994573542173	2.8054946304327557
>100	0.09927972327722985	7.828112532947716
>500	0.017524851562632025	4.591544688197318
>1k	0.014951691545639936	11.118166198599997
>5k	0.001079219327568055	2.65976226524117
>10k+	5.845771357660298E-4	5.192475322305599
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	72925	0.6123678402296406	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	60050	0.5042535317900572	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	53197	0.4467073294027589	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	46211	0.38804429571274496	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	32635	0.2740435305573442	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30095	0.25271457184382634	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	28346	0.2380278203517229	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	26203	0.22003256109067226	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	26171	0.2197638497997933	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	21973	0.1845122873276091	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19092	0.16031987392066233	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	18362	0.15418989759748594	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	17533	0.14722859571815278	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16736	0.14053600512969855	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	16374	0.13749620865163026	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	14989	0.12586604809327506	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	13969	0.11730087569650806	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	13810	0.11596571646995321	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	13666	0.11475651566099786	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	12777	0.10729138011126663	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	12755	0.10710664109878734	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.1146416415841473E-4	0.0	0.0	0.0	0.0
2	4.1146416415841473E-4	0.0	0.0	0.0	0.0
3	4.2825861983835E-4	0.0	0.0	0.0	0.0
4	4.2825861983835E-4	0.0	0.0	0.0	0.0
5	4.5345030335825295E-4	2.5191683519902943E-5	0.0	8.397227839967648E-6	0.0
6	4.618475311982206E-4	2.5191683519902943E-5	0.0	8.397227839967648E-6	0.0
7	4.618475311982206E-4	2.5191683519902943E-5	0.0	8.397227839967648E-6	0.0
8	4.7024475903818824E-4	2.5191683519902943E-5	0.0	1.6794455679935295E-5	0.0
9	4.7024475903818824E-4	2.5191683519902943E-5	0.0	5.878059487977353E-5	0.0
10	4.786419868781559E-4	2.5191683519902943E-5	0.0	1.931362403192559E-4	0.0
11	4.786419868781559E-4	3.358891135987059E-5	0.0	2.0153346815922354E-4	0.0
12	4.786419868781559E-4	3.358891135987059E-5	0.0	2.7710851871893237E-4	0.0
13	4.786419868781559E-4	3.358891135987059E-5	0.0	2.7710851871893237E-4	0.0
14	4.786419868781559E-4	4.1986139199838235E-5	0.0	3.023002022388353E-4	0.0
15	4.870392147181235E-4	4.1986139199838235E-5	0.0	3.2749188575873823E-4	0.0
16	4.870392147181235E-4	5.0383367039805886E-5	0.0	4.030669363184471E-4	0.0
17	6.129976323176382E-4	5.0383367039805886E-5	0.0	4.450530755182853E-4	0.0
18	6.129976323176382E-4	5.0383367039805886E-5	0.0	4.954364425580912E-4	0.0
19	6.549837715174765E-4	5.0383367039805886E-5	0.0	5.290253539179618E-4	0.0
20	6.549837715174765E-4	5.0383367039805886E-5	0.0	5.878059487977353E-4	0.0
21	6.717782271974118E-4	5.0383367039805886E-5	0.0	6.213948601576059E-4	0.0
22	6.801754550373794E-4	5.878059487977353E-5	0.0	6.969699107173147E-4	0.0
23	6.969699107173147E-4	6.717782271974118E-5	0.0	0.001024461796476053	0.0
24	7.221615942372176E-4	6.717782271974118E-5	0.0	0.0012931730873550176	0.0
25	7.473532777571206E-4	6.717782271974118E-5	0.0	0.0014191315049545323	0.0
26	7.893394169569589E-4	6.717782271974118E-5	0.0	0.0016626511123135942	0.0
27	7.893394169569589E-4	8.397227839967647E-5	0.0	0.0019901429980723325	0.0
28	8.061338726368942E-4	9.236950623964411E-5	0.0	0.003014604794548385	0.0
29	8.229283283168295E-4	9.236950623964411E-5	0.0	0.004030669363184471	0.0
30	8.733116953566353E-4	9.236950623964411E-5	0.0	0.005878059487977353	0.0
31	8.985033788765383E-4	9.236950623964411E-5	0.0	0.008187297143968457	0.0
32	8.985033788765383E-4	9.236950623964411E-5	0.0	0.010639287673239009	0.0
33	9.656812015962794E-4	9.236950623964411E-5	0.0	0.013326400582028657	8.397227839967648E-6
34	9.74078429436247E-4	9.236950623964411E-5	0.0	0.01667689449017575	8.397227839967648E-6
35	9.74078429436247E-4	9.236950623964411E-5	0.0	0.020993069599919117	8.397227839967648E-6
36	9.824756572762148E-4	9.236950623964411E-5	0.0	0.02548558649430181	8.397227839967648E-6
37	9.9927011295615E-4	9.236950623964411E-5	0.0	0.031985040842436765	8.397227839967648E-6
38	0.0010076673407961177	9.236950623964411E-5	0.0	0.04013874907504535	8.397227839967648E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGAG	1385	0.0	20.654781	24
TGCGACG	1495	0.0	18.692825	22
AATGCGA	1560	0.0	18.334425	20
CAATGCG	1580	0.0	17.545275	19
GACGAGT	1580	0.0	17.408512	25
ATCGTCA	1650	0.0	16.795494	10
TCGACGA	1515	0.0	16.55245	37
GTACTAG	1970	0.0	16.086298	1
GTCGACG	1520	0.0	16.063843	36
CGTCGTA	2770	0.0	16.03902	10
CCGTCGG	2765	0.0	15.996609	24
CGACGAA	1610	0.0	15.986849	38
GACCGTC	2865	0.0	15.975376	22
CCGTCGT	2840	0.0	15.877557	9
CATTGCG	1640	0.0	15.8291445	29
GTGATCG	1980	0.0	15.77633	8
CAATTCG	1705	0.0	15.612453	15
GTCGGCT	2875	0.0	15.461104	26
CGAACAA	1780	0.0	15.448865	41
TGATCGC	2015	0.0	15.3919	9
>>END_MODULE