FastQCFastQC Report
Wed 25 May 2016
SRR2558171_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2558171_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16307640
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA327810.20101621080671392No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269940.1655297762275841No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC267140.163812789588193No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA263500.1615807069569846No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT241240.14793066317382528No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT229650.14082356490577425No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA209950.12874333747862965No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA200830.12315086671032718No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG178990.10975837092307655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGA24100.017.61691937
GTACTAG22400.017.5860881
CGTCATT26750.017.27469626
GTCGACG24500.017.23950636
CGACGAA25450.016.94251638
CATTGCG28150.016.56798729
TAGGTCG26450.016.5541821
AGGTCGT26600.016.54481722
CAATTCG26900.016.35663815
AATGCGA20350.016.21752220
CGTCGTA53200.015.9990110
CCGTCGT53500.015.9929849
TGCGACG19900.015.92293222
GTGATCG29250.015.7932838
CAATGCG21100.015.74510119
GTCGTCA28750.015.69086724
TACCGTC57150.015.5892717
GCTCGTC31300.015.32231939
CAATTAG30250.015.20094417
AGTCGAC27600.015.14321935
Produced by FastQC (version 0.11.5)