FastQCFastQC Report
Wed 25 May 2016
SRR2558169_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2558169_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26141498
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2551740.9761261577282221No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1665180.636987214734213No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1300200.49737012010558845No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1183450.45270932828715477No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1022180.39101814287765757No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG467250.17873880066092618No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG464440.17766388138889364No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT414820.15868256669912337No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG399500.15282215273202784No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT342520.1310253911233396No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC301420.11530326226905588No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT273030.10444313481958838No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC268700.10278676455343148No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC266830.10207142681723902No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA264140.1010424115710584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG37350.020.6151824
CAATGCG36800.020.38447819
TGCGACG38450.019.79650922
AATGCGA38350.019.73332420
GTACTAG45750.019.1878911
GACGAGT43850.018.06103525
TCTAGAC85050.016.8139743
GTCGTCA35500.016.79386324
GTATCAA1947350.016.7289431
CTAGACA89750.016.5469234
GTCGACG35000.016.15389436
TCGACGA35850.015.70931237
GTTCTAG95400.015.6360391
CTAGGCA68000.015.4656064
TAGACAT95550.015.404265
CTCTAGT82900.015.36327627
AGTCCTA51750.015.30183929
GTAGACC87000.015.1985854
TAGGTCG39600.015.1106621
ATCGTCA51000.015.0991210
Produced by FastQC (version 0.11.5)