FastQCFastQC Report
Wed 25 May 2016
SRR2558168_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2558168_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16219910
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1024650.6317236038917602No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT912770.5627466490257961No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT803740.4955267939217912No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT546190.33674046280158154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT427590.2636204516547872No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG403980.24906426731097767No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG380930.2348533376572373No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC363370.22402713701863944No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA333510.20561766372316492No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG326250.2011416832769109No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC241160.14868146617336347No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC236930.14607356021087664No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC226410.13958770424743416No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT226100.13939658111543157No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT165620.1021090745879601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACG16250.017.5995422
GTACTAG22200.017.4444851
AATGCGA18000.016.13286220
TCGACGA14250.015.59233537
CGACGAA14250.015.43809838
TAGGTCG17550.015.41849621
GTCGACG14700.015.26481336
CAATGCG18750.015.13499819
CGACGAG21350.014.94124724
CTAACGC12850.014.5525983
AGTCGAC15750.014.52651635
TCTAACG12000.014.2983482
TCTAGAC28000.014.2214973
CATTGCG18800.014.04015129
CGCGAAT7550.013.98630435
GTCGTCA21150.013.52227324
CTAGACA32850.013.3948584
GTATTAG25800.013.3899341
CTAGTAC29300.013.2901663
ACGCGAA7950.013.28226334
Produced by FastQC (version 0.11.5)